The effect of nitrogen, phosphorus and potassium nutrition on average seed weight, oil content and fatty acid composition of rape seed (Brassica napus) grown in soil‐free culture has been studied. Nitrogen effected an increase in seed weight and a decrease in oil content, while the average amount of oil per seed remained constant. A small, but highly significant, decrease in palmitic and eicosenoic acid content, a significant decrease in oleic acid and a highly significant increase in erucic acid content were observed. This suggests that a decrease in the extent of elongation of oleic acid to erucic acid occurs in seeds developing on plants with sub‐optimal levels of nitrogen nutrition. Phosphorus and potassium had very limited effects on fatty acid composition. Significant differences were found only in oleic acid content for phosphorus alone, the nitrogen‐phosphorus interaction and the phosphorus‐potassium interaction. The effect of various levels of sulfate at optimal levels for nitrogen, phosphorus and potassium, was studied in a separate experiment. Seed from sulfur‐starved plants had decreased oil content; oleic acid percentages were increased and erucic acid percentages decreased. Excessive amounts of sulfate had no effect on fatty acid composition.