Effects of ionizing radiation and HPSE1 inhibition on the invasion of oral tongue carcinoma cells on human extracellular matrices in vitro

Väyrynen, Otto; Piippo, Markku; Jämsä, Hannaleena; Väisänen, Tuomas Lauri Aleksanteri; Almeida, Carlos Eduardo Bonacossa de; Salo, Tuula; Missailidis, Sotiris; Risteli, Maija

doi:10.1016/j.yexcr.2018.08.005

Cited by 9 publications

(6 citation statements)

References 47 publications

(93 reference statements)

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“…Since significant differences were observed in myoma discs between different cell lines, we further wanted to investigate the impact of ionized radiation to cell invasion. We have shown in the previous myoma experiments that 4 Gy radiation is optimal dose to for the major changes in various oral carcinoma cell lines invasion [30]. Here we detected that the invasion depth was significantly reduced in all experiments with dose of 4 Gy, which was in line with our previous experiment [30].…”

Section: Discussionsupporting

confidence: 91%

“…We have shown in the previous myoma experiments that 4 Gy radiation is optimal dose to for the major changes in various oral carcinoma cell lines invasion [30]. Here we detected that the invasion depth was significantly reduced in all experiments with dose of 4 Gy, which was in line with our previous experiment [30]. We also showed that ionized radiation affected to the total invasion area, and the invasion index decreased clearly in all of the cells studied: 27.1% in HSC-3, 7.6% in SiHa, and 3.5% in CaSki cells.…”

Section: Discussionmentioning

confidence: 93%

“…We have introduced gelatinous Myogel Transwell invasion assay, which has proved to be a fast method for cancer cells invasion experiments [1,3,6,7,30]. Carcinoma cells invade faster in Myogel than in the traditionally used commercial Matrigel® [4,6].…”

Section: Discussionmentioning

confidence: 99%

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Human myoma tissue-based extracellular matrix models for testing the effects of irradiation on the HPV positive cells

Tuominen

Al‐Samadi

Salo

et al. 2020

Virol J

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Background: This study was designed to investigate the invasion of human papillomavirus (HPV) positive human cervical carcinoma cell lines in human leiomyoma-based extracellular matrices in vitro, and to test the suitability of the model for studying the irradiation effects on the cancer cell invasion. Methods: HPV positive cervical carcinoma cell lines SiHa and CaSki, and HPV negative squamous cell carcinoma cell line HSC-3 were used. CaSki cells contain around 600 copies of HPV 16 virus in the genome, whereas SiHa have only 1-2 copies per cell. Cells were analyzed using two different human tumor derived extracellular matrix methods (3D myoma disc model, and Myogel Transwell invasion assay). Cultures were irradiated with 4 Gy. Myoma invasion area and the depth of invasion were measured with ImageJ 1.51j8 software. Statistical analyses were performed with SPSS Statistics (IBM SPSS® Statistics 25). Results: All cells invaded through Myogel coated Transwell membranes and within myoma discs. In myoma discs, a difference in the invasion depth (p = 0.0001) but not in invasion area (p = 0.310) between the HPV positive cell lines was seen, since SiHa (less HPV) invaded slightly better than CaSki (more HPV). HSC-3 cells (HPV negative) invaded deepest (p = 0.048) than either of the HPV positive cell line cells. No difference was detected in the invasion area (p = 0.892) between HPV positive and HPV negative cells. The ionized radiation significantly reduced the invasion depth of HSC-3 (p = 0.008), SiHa (p = 0.0001) and CaSki (p = 0.005). No significant effect on the invasion area was detected in any of the cell lines. However, a significant difference was observed between SiHa and CaSki in the reduction of the invasion depth after radiation (p = 0.013) as the reduction was greater with SiHa than CaSki. Conclusions: Both solid and gelatinous human leiomyoma-based extracellular matrix models were suitable platforms to study the invasion of HPV positive cervical carcinoma cells in vitro. SiHa cells with less HPV copy number cells invaded slightly better and were slightly more sensitive to irradiation than CaSki cells with high HPV copy number. However, there was no drastic differences between the invasion properties of these carcinoma cells.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 93%

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Human myoma tissue-based extracellular matrix models for testing the effects of irradiation on the HPV positive cells

Tuominen

Al‐Samadi

Salo

et al. 2020

Virol J

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“…(36) Also in Myogel invasion, FWGE treated HSC-3 and SAS cells behaved similar as in spheroid and migration assays. This confirms the different aggressiveness of these cells, observed already in previous studies (37,38), and demonstrates that oral tongue carcinoma cells display variable phenotypes and respond differently on drugs and natural compounds, like FWGE. Our results are in line with some previously reported data, showing that Avemar® inhibits OTSCC cell migration and invasion.…”

Section: Discussionsupporting

confidence: 90%

Effects of Fermented Wheat Germ Extract on Oral Cancer Cells: An In Vitro Study

Zhurakivska

Risteli

Salo

et al. 2021

Nutrition and Cancer

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Oral carcinoma is one of the most aggressive cancers, and despite the advances in the therapy, its mortality is still high. An attention in cancer treatment has focused on natural compounds due to their potential beneficial effects on human health. In this study, the effects of dietary supplement Fermented Wheat Germ Extract (FWGE) on oral tongue squamous cell carcinoma (OTSCC) cells were investigated in vitro using three cell lines (HSC-3, SAS, SCC-25) with variable aggressiveness.The cell viability was significantly decreased by the treatment with high concentration of FWGE in every cell line. Regarding migration and invasion, HSC-3 and SCC-25 cells were most sensitive to FWGE since their movement was significantly reduced with 5 and 10 mg/ml FWGE, while SAS was inhibited only with 10 mg/ml FWGE. Chemotherapeutic compounds (cisplatin and 5-fluorouracil) significantly reduced all OTSCC cells viability. Importantly, combination of these drugs with 10 mg/ml FWGE significantly decreased the cell viability compared to the treatment with the chemotherapeutics or FWGE alone. Based on these in vitro experiments, the use of FWGE seems to improve the anticancer effects on OTSCC cells. Further in vivo and clinical studies should be conducted to verify the positive effects of FWGE for OTSCC patients.

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“…The enzyme recognition by the aptamer is in some cases superior over polyclonal anti-heparanase antibody (Simmons et al., 2012). Furthermore, a short anti-heparanase aptamer (30 bases) inhibits tissue invasion, does not exhibit cytotoxicity on oral carcinoma cells, and is stable in human serum without significant binding to serum proteins suggesting, the potential application of aptamers as therapeutic agents (Simmons et al., 2014, Vayrynen et al., 2018).…”

Section: Nucleic Acid-based Inhibitorsmentioning

confidence: 99%

Targeting Heparanase in Cancer: Inhibition by Synthetic, Chemically Modified, and Natural Compounds

et al. 2019

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Heparanase is an endoglycosidase involved in remodeling the extracellular matrix and thereby in regulating multiple cellular processes and biological activities. It cleaves heparan sulfate (HS) side chains of HS proteoglycans into smaller fragments and hence regulates tissue morphogenesis, differentiation, and homeostasis. Heparanase is overexpressed in various carcinomas, sarcomas, and hematological malignancies, and its upregulation correlates with increased tumor size, tumor angiogenesis, enhanced metastasis, and poor prognosis. In contrast, knockdown or inhibition of heparanase markedly attenuates tumor progression, further underscoring the potential of anti-heparanase therapy. Heparanase inhibitors were employed to interfere with tumor progression in preclinical studies, and selected heparin mimetics are being examined in clinical trials. However, despite tremendous efforts, the discovery of heparanase inhibitors with high clinical benefit and minimal adverse effects remains a therapeutic challenge. This review discusses the key roles of heparanase in cancer progression focusing on the status of natural, chemically modified, and synthetic heparanase inhibitors in various types of malignancies.

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Effects of ionizing radiation and HPSE1 inhibition on the invasion of oral tongue carcinoma cells on human extracellular matrices in vitro

Cited by 9 publications

References 47 publications

Human myoma tissue-based extracellular matrix models for testing the effects of irradiation on the HPV positive cells

Human myoma tissue-based extracellular matrix models for testing the effects of irradiation on the HPV positive cells

Effects of Fermented Wheat Germ Extract on Oral Cancer Cells: An In Vitro Study

Targeting Heparanase in Cancer: Inhibition by Synthetic, Chemically Modified, and Natural Compounds

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