Effects of ionizing irradiation and interface backscatter on human mesenchymal stem cells cultured on titanium surfaces

Printzell, Lisa; Reseland, Janne Elin; Edin, Nina Frederike Jeppesen; Ellingsen, Jan Eirik

doi:10.1111/eos.12654

Cited by 5 publications

(9 citation statements)

References 27 publications

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“…The IL-6 transcripts were upregulated at 3 up to 14 days in the IrI and IrL groups. IL-6 was secreted by hMSCs at day 1 after irradiation and started declining at day 7 until day 21, regardless of the dose and implant surface [29]. The release of IL-6 and GM-CSF contributes to pathological bone resorption by stimulating the differentiation of mononuclear phagocyte osteoclast precursors into mature bone-resorbing cells [10,30].…”

Section: Discussionmentioning

confidence: 99%

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Effect of radiotherapy on the differentiation and osteogenic activity of mesenchymal stem cells on dental implants

et al. 2023

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Objective: to evaluate the differentiation and gene expression of transcripts related to osteogenesis in a primary culture of Mesenchymal Stem Cells (MSCs) derived from rat femurs submitted to radiotherapy and the installation of pure titanium implants. Material and Methods: fifty-four rats received titanium implants in both femurs and were divided into three groups: Control: implant surgery (C); Implant + immediate irradiation (IrI), and Implant + late irradiation (IrL). Euthanasia occurred 3, 14, and 49 days after surgery. The bone marrow MSCs from the femurs were isolated and cultivated. The cell viability, total protein content, alkaline phosphatase (ALP) activity, and the formation of mineralization nodules and cellular genotoxicity were analyzed. The gene expression of Alkaline Phosphatase (phoA), Collagen 1 (COL1), Runt-related transcription factor 2 (RUNX2), Osterix (OSX), Osteopontin (OPN), Integrin β 1 (ITGB 1 ), Bone Sialoprotein (BSP), Osteonectin (SPARC), Osteocalcin (Bglap), Transforming Growth Factor β-type (TGF-β), Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), Interleukin-6 (IL-6), Apolipoprotein E (APOE) and Prostaglandin E 2 synthase (PGE 2 ) were evaluated by qRT-PCR. Results: ionizing radiation suppresses the gene expression of essential transcripts for bone regeneration, as well as cellular viability, as observed in the IrI and IrL groups. Conclusion: although this can lead to the loss of osseointegration and failure of the implant, the MSCs showed more activity at 49 days than at 3 and 14 days.

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Section: Discussionmentioning

confidence: 99%

“…In bone marrow, PGE2 production has been reported not to reach a maximum until several days after irradiation [31] as we demonstrated. A viable stem cell will naturally produce high levels of immune-response factors after any trauma or external influence, such as irradiation [29].…”

Section: Discussionmentioning

confidence: 99%

Effect of radiotherapy on the differentiation and osteogenic activity of mesenchymal stem cells on dental implants

et al. 2023

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“…Endostatin can specifically act on endothelial cells during neovascularization. It can also have an antiangiogenic effect by regulating the expression of VEGF and the activity of proteolytic enzymes on the surface of tumor cells, which indirectly leads to tumor dormancy or retraction[ 15 , 16 ]. The advantage of antiangiogenic therapy is that it can be targeted toward pathological blood vessels and tumor blood vessels, and resistance to antiangiogenic therapy does not easily develop, mainly because the vascular endothelial genome is relatively stable.…”

Section: Discussionmentioning

confidence: 99%

Endu combined with concurrent chemotherapy and radiotherapy for stage IIB-IVA cervical squamous cell carcinoma patients

Zhao¹,

Su²,

Zhang³

et al. 2021

WJCC

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BACKGROUND In recent years, the incidence of cervical cancer has increased with increasing life pressures and changes in women's social roles, posing a serious threat to women's physical and mental health. AIM To explore the clinical effect of Endo combined with concurrent radiotherapy and chemotherapy in the treatment of advanced cervical squamous cell carcinoma. METHODS A total of 120 patients admitted to the oncology department of our hospital were selected as the research subjects. They were equally divided into the test group and the control group (60 patients each) with a random number table. The test group was treated with Endo combined with concurrent radiotherapy and chemotherapy, and the control group was treated with concurrent radiotherapy and chemotherapy. We compared the serum thymidine kinase 1 (TK1), human epididymis protein 4 (HE4), vascular endothelial growth factor (VEGF), and squamous cell carcinoma-associated antigen (SCC-Ag) levels, the clinical effects and survival before and after radiotherapy and chemotherapy, the quality score, and the 3-year follow-up outcomes between the two groups. RESULTS After chemotherapy, the complete remission + partial remission rate was 85.00% in the test group and 68.33% in the control group; the difference was not statistically significant ( P > 0.05). Before chemotherapy, the serum TK1, HE4, VEGF, and SCC-Ag levels of the two groups were not significantly different ( P > 0.05). After chemotherapy, the levels of serum TK1 (1.27 ± 0.40 pmol/L), HE4 (81.4 ± 24.0 pmol/L), VEGF (235.1 ± 38.0 pg/mL), and SCC-Ag (1.76 ± 0.55 ng/mL) were lower than those in the control group [TK1 (1.58 ± 0.51 pmol/L), HE4 (98.0 ± 28.6) pmol/L, VEGF (284.2 ± 54.1 pg/mL), and SCC-Ag (2.34 ± 0.78 ng/mL)]. The difference was statistically significant ( P < 0.05). Before chemotherapy, there were no significant differences in the physical, role, mood, cognition, social and symptom scale scores of the two groups ( P > 0.05). After chemotherapy, the physical, role, mood, cognitive and social scores were higher in the test group than in the control group, and the difference was statistically significant ( P < 0.05). The symptom scale scores of the test group were all lower than those of the control group, and the difference was statistically significant ( P < 0.05). The 3-year progression-free survival (PFS) rate was 43.33% in the test group and 26.67% in the control group; the overall survival (OS) rate was 48.33% in the test group and 33.33% in the control group; the differences were not statistically significant ( P > 0.05). The 3-year PFS time of the test group was 20.0 mo, which was longer than that of the control group (15.0 mo), and the difference was significant ( P < ...

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“…Since then, several clinical trials have reported positive results on the primary placement protocol [ 30–33 ]. However, precautions must be taken as radiation backscatter from titanium has been shown to escalate the radiation dose to the cells growing on the surface with more than 40% [ 34 ]. Fortunately, backscattered radiation from titanium is of a very short range and cells at a distance of 1 mm from the surface receive only a 15% higher radiation dose due to backscatter [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…Osteoblasts play an essential role in bone regeneration and remodeling, and thus, in the osseointegration of dental implants [ 35 ]. While a previous report showed that the escalated radiation dose caused by backscatter from titanium did not influence the response of human mesenchymal stem cells significantly [ 34 ], OBs have demonstrated a radiation dose-dependent reduction in cell attachment and proliferation [ 36 , 37 ]. However, in vitro studies investigating the effects of irradiation on human OBs cultured on titanium are very limited.…”

Section: Introductionmentioning

confidence: 99%

The dose-dependent impact of γ-radiation reinforced with backscatter from titanium on primary human osteoblasts

Printzell

Reseland

Edin

et al. 2023

Biomaterial Investigations in Dentistry

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In head and neck cancer patients receiving dental implants prior to radiotherapy, backscatter from titanium increases the radiation dose close to the surface, and may affect the osseointegration. The dose-dependent effects of ionizing radiation on human osteoblasts (hOBs) were investigated. The hOBs were seeded on machined titanium, moderately rough fluoride-modified titanium, and tissue culture polystyrene, and cultured in growth- or osteoblastic differentiation medium (DM). The hOBs were exposed to ionizing γ-irradiation in single doses of 2, 6 or 10 Gy. Twenty-one days post-irradiation, cell nuclei and collagen production were quantified. Cytotoxicity and indicators of differentiation were measured and compared to unirradiated controls. Radiation with backscatter from titanium significantly reduced the number of hOBs but increased the alkaline phosphatase activity in both types of medium when adjusted to the relative cell number on day 21. Irradiated hOBs on the TiF-surface produced similar amounts of collagen as unirradiated controls when cultured in DM. The majority of osteogenic biomarkers significantly increased on day 21 when the hOBs had been exposed to 10 Gy, while the opposite or no effect was observed after lower doses. High doses reinforced with backscatter from titanium resulted in smaller but seemingly more differentiated subpopulations of osteoblasts.

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Effects of ionizing irradiation and interface backscatter on human mesenchymal stem cells cultured on titanium surfaces

Cited by 5 publications

References 27 publications

Effect of radiotherapy on the differentiation and osteogenic activity of mesenchymal stem cells on dental implants

Effect of radiotherapy on the differentiation and osteogenic activity of mesenchymal stem cells on dental implants

Endu combined with concurrent chemotherapy and radiotherapy for stage IIB-IVA cervical squamous cell carcinoma patients

The dose-dependent impact of γ-radiation reinforced with backscatter from titanium on primary human osteoblasts

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