Effects of ionic liquid and nanogold particles on high-performance liquid chromatography-electrochemical detection and their application in highly efficient separation and sensitive analysis of five phenolic acids in Xuebijing injection

Jia, Ping; Wang, Shixiang; Xue, Ming; Lan, Wei; Luo, Jing; Liao, Sha; Xiao, Chaoni; Zheng, Xuemei; Li, Lianshe; Liu, Qinshe; Zheng, Jianbin; Zhou, Yuanzhen; Zheng, Xiaohui

doi:10.1016/j.talanta.2012.12.031

Cited by 33 publications

(18 citation statements)

References 40 publications

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“…This study investigated the pharmacokinetic analysis of single and combined medication of PAL and HSYA, which are the 2 main parts of DHI. The previous studies have shown the pharmacokinetics of PAL and HSYA by using them either alone or combined with other drugs in normal rats [24][25][26]. The corresponding mechanism of the single and combined application in hyperlipidemia model, however, has not been reported.…”

Section: Discussionmentioning

confidence: 96%

Pharmacokinetic Study on Protocatechuic Aldehyde and Hydroxysafflor Yellow A of Danhong Injection in Rats with Hyperlipidemia

Zhou¹,

Li²,

Jin³

et al. 2018

Pharmacology

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Background: Protocatechuic aldehyde (PAL) and hydroxysafflor yellow A (HSYA) are 2 effective ingredients of Danhong Injection, which is extensively used for the clinical treatment of cardio-cerebrovascular diseases. This study aims to investigate the pharmacokinetic differences between single and combined medication of PAL and HSYA and analyze the interaction of the above effective components in hyperlipidemia rats. Methods: Thirty male SD rats were randomly divided into the control group (n = 6) and the model group (n = 24). The hyperlipidemia model was established by feeding with superfatted forage. The successful model rats were then randomly divided into the PAL group (16 mg/kg), the HSYA group (10 mg/kg), and the combination group (16 mg/kg + 10 mg/kg). Administration through tail-vein, and orbital blood was sampled at different time points. The mass concentration of PAL and HSYA was determined by high performance liquid chromatography (HPLC-DAD). Analysis of pharmacokinetic parameters was conducted by using DAS 3.2.6 software and SPSS 19.0 statistical analysis software. Results: According to the parameters of statistical moment of non-compartmental model, there was a significant difference in plasma clearance (CL) between the PAL group and the drug combination group (p < 0.01), as well as in the area under the first moment of the plasma concentration-time curve and the elimination half-life (t_1/2) between the HSYA group and the drug combination group (p < 0.01) but no obvious differences about the blood concentration time curve area, the average dwell time (MRT), and the peak concentration (C_max; p > 0.05). Conclusion: The combined medication of PAL and HSYA could increase the plasma CL significantly and have a great influence on the absorption of HSYA in rats with hyperlipidemia.

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Section: Discussionmentioning

confidence: 96%

Pharmacokinetic Study on Protocatechuic Aldehyde and Hydroxysafflor Yellow A of Danhong Injection in Rats with Hyperlipidemia

Zhou¹,

Li²,

Jin³

et al. 2018

Pharmacology

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“…29,30 In order to guarantee the efficacy of XBJ injection, some research has been done on both qualitative and quantitative analysis of the chemical composition of XBJ. [31][32][33][34] Most of these studies have been focused on better quality control of XBJ injection through different detection methods. For example, Bai group applied the time-of-flight (TOF) MS or bioactivity-integrated quadrupole (Q)/TOF methods; 31,32 Zheng and co-workers 33 developed the method of electrochemical detection with IL and nanogold particles as additives; while the group of Zhang and co-workers 34 operated the detection with high resolution ultra HPLC hybrid quadruple-orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS).…”

Section: Introductionmentioning

confidence: 99%

Study on the Mixed-Mode HPLC Separation of Shuang-Huang-Lian and Xue‑Bi‑Jing Injections Based on an Ionic Liquid Column

Yang¹,

Zhang²,

Zhang³

et al. 2018

J. Braz. Chem. Soc.

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Both Shuang-Huang-Lian and Xue-Bi-Jing injections are clinically proven efficient and low cost medicines. However, the studies of their chromatographic separation are limited. In this study, a column based on ionic liquid stationary phase was applied for the first time for the analysis of these two injections. For Shuang-Huang-Lian injection, U-shaped plots of retention factors vs. mobile phase constitutions were obtained. This unique kind of separation behavior allowed a flexible adjustment of retention by tuning the mobile phase constituents. Then acids group was analyzed with hydrophilic chromatography mode, while glycosides group was analyzed with reversed phase chromatography mode. Similarly, constituents in Xue-Bi-Jing injection were analyzed with mixed mode high performance liquid chromatography (HPLC). Higher, medium and lower polarity groups were analyzed with hydrophilic interaction, reversed phase and normal phase liquid chromatography modes, respectively. Results from this work provided valuable information for the separation of complicated systems such as traditional Chinese medicine injections.Keywords: ionic liquid stationary phase, mixed-mode chromatography, Shuang-Huang-Lian injection, Xue-Bi-Jing injection IntroductionThe formulations of traditional Chinese medicines (TCMs) are becoming increasingly popular worldwide, mainly because of their high efficiency and low cost. However, TCM formulations are usually based on a whole plant and/or combination of several herbs, which contain more than dozens to hundreds of components. 1 Therefore, in order to ensure both the safety and efficacy of TCM formulations, it is necessary to establish simple and effective methods to determine as many constituents as possible.2,3 Liquid chromatography (LC) has been applied as a rapid and economic method for the determination of multi-component in TCMs. 4 Among the liquid chromatography analytical methods, high performance liquid chromatography (HPLC) is the most popular one, due to its easy operation, wide suitability and high accuracy for the qualitative and quantitative analysis of TCMs. [5][6][7] However, in practice, some factors, such as efficiency, selectivity and sensitivity have been found to affect the analysis of complicated systems with HPLC. 8Chromatographic stationary phase is the core of HPLC system. Therefore, the development of novel stationary phases with high separation efficiency and excellent selectivity has always been the focus point of improving the separating ability of HPLC. Accordingly, in this work novel ionic liquid (IL) bonded stationary phase has been studied. IL is a kind of novel material composed of ions and at the same time remains in liquid state at a temperature below 100 °C.9 ILs have demonstrated the versatility and tuneability in separation science. 10 Many types of ILs were immobilized on a support like silica or monolith as stationary phases for liquid chromatography. 11,12 Due to the designable character of the IL molecule, multi interactions between the IL stationary phas...

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“…In this method, the electrolyte flow through the electrode surface. According to its high mass transfer rate and refresh electrode surface, the flowing electrolysis has been used for waste water treatment [32,33], electrochemical detector [34,35] and reaction kinetics study [36][37][38]. Herein, a novel flowing electrolysis method was developed for the preparation of carbon nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

Flowing electrolytic synthesis of fluorescent carbon nanoparticles and carbon nanosheets

Shen

2015

Electrochimica Acta

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Effects of ionic liquid and nanogold particles on high-performance liquid chromatography-electrochemical detection and their application in highly efficient separation and sensitive analysis of five phenolic acids in Xuebijing injection

Cited by 33 publications

References 40 publications

Pharmacokinetic Study on Protocatechuic Aldehyde and Hydroxysafflor Yellow A of Danhong Injection in Rats with Hyperlipidemia

Pharmacokinetic Study on Protocatechuic Aldehyde and Hydroxysafflor Yellow A of Danhong Injection in Rats with Hyperlipidemia

Study on the Mixed-Mode HPLC Separation of Shuang-Huang-Lian and Xue‑Bi‑Jing Injections Based on an Ionic Liquid Column

Flowing electrolytic synthesis of fluorescent carbon nanoparticles and carbon nanosheets

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