Effects of iodipamide on human C3 and factor B in vitro

Effect of Metrizamide, a Nonionic Radiographic Contrast Agent, on Human Serum Complement

“…However, cleavage of C3 was also observed in the presence of EDTA, and occurred, therefore, independently of se quential activation of the classical or alternative path way [Till el al., 1978;Kolbel al., 1978;Arroyave el al., 1979]. Furthermore, the pattern observed in immu noelectrophoresis was different from that of serum activated with cobra venom factor or zymosan [Hasselbacher and Hahn, 1980;Durda et al, 1982;person al observations]. Our present studies give evidence that C3 in NHS previously exposed to ionic RCM al so differs in electrophoretic mobility from C3 in N^H^treated NHS that is known to contain cleaved C3b-like C3 [von Zabern et al, 1981a].…”

“…Depres sion of C2, C4, C3, factor B and P activities has been found to occur in serum after treatment with ionic RCM in the absence as well as in the presence of ED TA [Title t al., 1978;Kolb el al., 1978;Arroyave el al., 1979]. Some ionic RCM exert a direct effect on C3, C4 and factor B [Durda et al, 1982;von Zabern et al, 1983]. Our previous studies suggest that the exposure of purified C3 and C4 to ionic RCM caused specific alterations in these molecules [von Zabern et al, 1983].…”