Effects of Intravenous Infusion of Ascorbate on Common Clinical Chemistry Tests

Badrick, Tony; Campbell, Bruce

doi:10.1093/clinchem/38.10.2160a

Cited by 16 publications

(6 citation statements)

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“…26,33 Serum concentrations of ascorbate have been documented as high as 30 mmol/L (5,284 µg/mL). 28 We tested a spectrum of ascorbic acid levels up to 300 µg/mL, based on published observations, 33 to simulate most clinical scenarios. The results showed that at high concentrations, ascorbic acid can interfere with glucose measurements on all tested devices.…”

Section: Clinical Relevancementioning

confidence: 99%

Effects of Drugs on Glucose Measurements With Handheld Glucose Meters and a Portable Glucose Analyzer

Tang

Louie

et al. 2000

American Journal of Clinical Pathology

199

145

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Thirty drugs used primarily in critical care and hospital settings were tested in vitro to observe interference on glucose measurements with 6 hand-held glucose meters and a portable glucose analyzer. Paired differences of glucose measurements between drug-spiked samples and unspiked control samples were calculated to determine bias. A criterion of +/- 6 mg/dL was used as the cutoff for interference. Ascorbic acid interfered with the measurements on all glucose devices evaluated. Acetaminophen, dopamine, and mannitol interfered with glucose measurements on some devices. Dose-response relationships help assessment of drug interference in clinical use. High dosages of these drugs may be given to critically ill patients or self-administered by patients without medical supervision. Package inserts for the glucose devices may not provide adequate warning information. Hence, we recommend that clinicians choose glucose devices carefully and interpret results cautiously when glucose measurements are performed during or after drug interventions.

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Section: Clinical Relevancementioning

confidence: 99%

Effects of Drugs on Glucose Measurements With Handheld Glucose Meters and a Portable Glucose Analyzer

Tang

Louie

et al. 2000

American Journal of Clinical Pathology

199

145

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“…Plasma concentration of vitamin C is below 0.09 mmol/L in people with adequate intake of vitamin C, while high doses of vitamin C administration can raise the plasma vitamin C concentration as high as 30 mmol/L. 1 Ascorbic acid, due to its strong reducing activity, interferes signi¢cantly with chemical analyses involving oxidative reaction of chromogen reagents by peroxidase and hydrogen peroxide. 2,3 High dose vitamin C can falsely decrease bilirubin, glucose and cholesterol levels and increase creatinine levels.…”

Section: Introductionmentioning

confidence: 99%

Interference of ascorbic acid with chemical analytes

et al. 2005

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Background Ascorbic acid can interfere with methodologies involving redox reactions, while comprehensive studies on main chemistry analysers have not been reported. We therefore attempted to determine the interference of ascorbic acid with analytes on the Beckman Synchron LX20 s. Methods Various concentrations of ascorbic acid were added to serum, and the serum analytes were measured on the LX20. Results With a serum ascorbic acid concentration of 12.0 mmol/L, the values for sodium, potassium, calcium and creatinine increased by 43%, 58%, 103% and 26%, respectively (Po0.01). With a serum ascorbic acid concentration of 12.0 mmol/L, the values for chloride, total bilirubin and uric acid decreased by 33%, 62% and 83%, respectively (Po0.01), and were undetectable for total cholesterol, triglyceride, ammonia and lactate. There was no definite influence of ascorbic acid on analytical values for total CO 2 , urea, glucose, phosphate, total protein, albumin, amylase, creatine kinase, creatine kinase-MB, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total iron, unbound iron-binding capacity or magnesium. Conclusions Ascorbic acid causes a false increase in sodium, potassium, calcium and creatinine results and a false decrease in chloride, total bilirubin, uric acid, total cholesterol, triglyceride, ammonia and lactate results.

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“…Although this method is very specific to the glucose, reducing substances such as uric acid, ascorbic acid and creatinine interfere with the peroxidase step of this procedure (Sharp 1972; Zoppi and Fenili 1980; White‐Stevens 1982; White‐Stevens and Stover 1982; Badrick and Campbel 1992; Tang et al . 2000; Min and Skibsted 2002; Anderson and Cockayne 2003; Meng et al .…”

Section: Introductionmentioning

confidence: 99%

Green Tea Phenols Interference in the Glucose Oxidase/Peroxidase Test

Shaukat¹,

Waqar

2011

Journal of Food Biochemistry

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Green tea extract showed false-negative results in the determination of glucose via glucose oxidase (GOD) test. This investigation was undertaken to verify and find out the precise mechanism underlying this interference by determining the reaction kinetics of production and reduction of end-point chromophore. The peroxidase step of the GOD test was found to be interfered and phenols of the green tea were the interfering compounds. Green tea interfered and exerted its influence in a dual fashion. A part of the interference was observed as a result of the reduction of the finally formed chromophore, whereas the other form of interference was due to its hydrogen peroxide/free radical-scavenging activity. Reducing potential and the free radicalscavenging activity of the phenols in the green tea is the basis of the falsenegative results of the glucose by GOD test.

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Effects of Intravenous Infusion of Ascorbate on Common Clinical Chemistry Tests

Cited by 16 publications

References 0 publications

Effects of Drugs on Glucose Measurements With Handheld Glucose Meters and a Portable Glucose Analyzer

Effects of Drugs on Glucose Measurements With Handheld Glucose Meters and a Portable Glucose Analyzer

Interference of ascorbic acid with chemical analytes

Green Tea Phenols Interference in the Glucose Oxidase/Peroxidase Test

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