Effects of Intravenous Heparin on the Plasma Lipoproteins in Primary Hypercholesteremic Xanthomatosis and Idiopathic Hyperlipemia

Effects of Heparin on the Lipoproteins in Hyperlipemia. An Electrophoretic Study of the Serum Alpha and Beta Lipoproteins After Their Separation by Fractionation of the Plasma Proteins or Ultracentrifugal Flotation 1

“…Of especial interest were the findings on chemical fractionation where after the injection of heparin the beta lipoproteins on the paper strip used for the analysis of Fraction I + II + III miioved with the speed of alpha-2 or alpha-I globulin, while the alpha lipoproteins on the paper strip used for the analysis of Fraction IV + V" nmoved faster than albumin. This l)rovided conclusive evidence that the pre-albumiin component which we had observed following the injection of heparin in the electrophoretic patterns of whole serum in idiopathic hyperlipemia (1,2) as well as in alimentary hyperlipemia (3) (2). On moving boundary electrophoresis we observed in both persons a decrease in the beta-1 globulin peak from normal to subnormal values together with an increase in the alpha-2 peak in one and in the alpha-I peak in the other.…”

“…Second, as shown in the present study, isolation of the two lipoproteins by ultracentrifugal flotation followed by paper electrophoresis and staining for proteins showed after the injection of heparin neither in alimentary nor in idiopathic hyperlipemia any change in the distribution of the proteins between alpha and beta lipoproteins. Third, chemical fractionation of the plasma proteins by Cohn's Method 10 and determination of the amounts of cholesterol and phospholipids present as alpha lipoproteins and beta lipoproteins in idiopathic hyperlipemia never revealed abnormally high amounts of lipids in the alpha lipoprotein fraction either after a single injection or after the long-term administration of heparin; whereas the elevated values for cholesterol and phospholipids in the beta lipoprotein fraction decreased (1,20). Fourth, heparin was found to exert no effect on beta lipoproteins with Sf values below 12 (21 (24).…”

“…On the other hand, in alimentary hyperlipemia and idiopathic hyperlipemia electrophoretic changes were observed. In the patterns obtained by moving boundary electrophoresis the changes consisted of a decrease in the beta-1 globulins to a subnormal value and the appearance of a new component ahead of albumin (1,2). On the basis of this observation it had been assumed that the pre-albumin component consisted of beta lipoproteins.…”

“…It had been found that in normal, strictly fasting individuals an intravenous injection of heparin caused no changes in the patterns obtained either by moving boundary electrophoresis or by paper electrophoresis (1)(2)(3)(4). On the other hand, in alimentary hyperlipemia and idiopathic hyperlipemia electrophoretic changes were observed.…”

Interaction Between Oleate and the Lipoproteins of Human Serum

“…The effects of heparin on the electrophoretic distribution of plasma proteins have been studied recently by a number of authors. Using the moving boundary technique, Niklilii (11) and Lever, Smith, and Hurley (12,13) noted that the administration of heparin to a lipemic individual resulted in a decrease of the quantity beta globulin and a compensatory increase in *the amount of alpha globulin or albumin, or, in some of the patients studied by Lever, Smith, and Hurley, the formation of a new pre-albumin component. These phenomena were attributed by the latter authors to an increase in the mobility of beta lipoprotein which caused it to appear in an abnormal position in the electrophoretic pattern.…”