Effects of Intravenous ATP on Tracheal Ciliary Activity, Airway Resistance and Haemodynamics in Rats

Saano, Veijo; Muranen, Antti; Nuutinen, Juhani; Karttunen, P; Silvasti, M

doi:10.1111/j.1600-0773.1990.tb00752.x

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Intravenous ATP prevented the decrease in tracheal ciliary beat frequency that occurs in the incised trachea in control anesthetized rats after 70 min (Saano et al, 1990). Luminal administration of ATP increased tracheal ciliary beat frequency, whereas adenosine reduced the ATP effects (Wong and Yeates, 1992).…”

Section: A Epithelial Cellsmentioning

confidence: 99%

Purinergic Signaling in the Airways

Burnstock

Brouns²,

Adriaensen³

et al. 2012

Pharmacol Rev

172

158

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Section: A Epithelial Cellsmentioning

confidence: 99%

Purinergic Signaling in the Airways

Burnstock

Brouns²,

Adriaensen³

et al. 2012

Pharmacol Rev

172

158

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“…In previous investigations [20] with samples from healthy and infected mucosa, the authors could demonstrate that ciliary beating frequency from infected samples normalized as soon as the excrete was removed by simple washing. At first mucosal explants were immersed in drug-free Locke-Ringer's solution for 10 min (basal value).…”

Section: Measurement Of Cbf In Vitromentioning

confidence: 99%

Influence of azelastine and some selected drugs on mucociliary clearance

U¹,

Saano

Minker³

et al. 1992

Lung

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The effect of azelastine and some selected compounds on ciliary beating frequency (CBF) was investigated in vitro using human mucosal samples and in vivo using anesthetized guinea pigs. Further influence of azelastine on mucus secretion was evaluated in mice and on mucociliary clearance in anesthetized rabbits. Azelastine influenced the ciliary beating frequency neither in vitro nor in vivo. Azelastine, similarly to salbutamol, ambroxol, and bromhexine, increased mucus secretion measured by the tracheal output of phenol red. Azelastine dose-dependently enhanced mucociliary clearance measured by elimination of 99mTc-labeled erythrocytes in rabbits. The activity of azelastine proved to be about 10 times stronger than that of bromhexine. Since the ciliary activity remained unchanged under the influence of azelastine, it is likely that azelastine increases the mucociliary clearance by enhancing bronchial secretion. It is possible that the observed increase in mucociliary clearance may contribute to the beneficial effect of azelastine in the treatment of respiratory diseases.

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Possible mechanism of ciliary stimulation by extracellular ATP: Involvement of calcium-dependent potassium channels and exogenous Ca2+

et al. 1992

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Ciliary motility was examined optically in tissue cultures from frog palate epithelium and frog's esophagus as a function of extracellular concentration of adenosine 5'-triphosphate (ATP) and related compounds. The addition of micromolar concentration of ATP caused a strong enhancement of frequency and wave velocity in the direction of the effective stroke. Since adenosine 5'-[beta,gamma imido]-triphosphate (AMP-PNP), a nonhydrolyzable analog of ATP, produces the same effects, ATP hydrolysis is not required. The overall potency is ATP approximately equal to AMP-PNP greater than ADP much greater than adenosine greater than AMP. It is suggested that both the phosphate and the base moieties are involved in ATP binding. The enhancement of ciliary activity by extracellular ATP is dependent on the presence of extracellular Ca2+, which can be replaced by extracellular Mg2+. The effect of a number of potent inhibitors of the voltage-gated calcium channels on the stimulation of ciliary activity by ATP were examined. No effect was detected in the concentration range within which these agents are specific. On the other hand, quinidine, a potent inhibitor of K+ (calcium-dependent) channels, inhibits the effect of ATP. The following model is suggested: exogenous ATP interacts with a membrane receptor in the presence of Ca2+, a cascade of events occurs which mobilizes intracellular calcium, thereby increasing the cytosolic free Ca2+ concentration which consequently opens the calcium-activated K+ channels, which then leads to a change in membrane potential. The ciliary response to these changes is the enhancement of ciliary activity.

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Effects of Intravenous ATP on Tracheal Ciliary Activity, Airway Resistance and Haemodynamics in Rats

Cited by 4 publications

References 18 publications

Purinergic Signaling in the Airways

Purinergic Signaling in the Airways

Influence of azelastine and some selected drugs on mucociliary clearance

Possible mechanism of ciliary stimulation by extracellular ATP: Involvement of calcium-dependent potassium channels and exogenous Ca2+

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