Effects of intraseptal injection of 192-IgG-saporin in mature and aged Long-Evans rats

Bannon, Anthony W.; Curzon, Peter; Gunther, Karen L.; Decker, Michael

doi:10.1016/0006-8993(95)01568-x

Cited by 39 publications

(25 citation statements)

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“…These rats were mavimal1y affected by the 1 hour delay, but at the In a separate study, injections of 800 ng of 192 IgG-saporin into the hippocampi of both mature and aged rats produced similar reductions of hippocampal ChAT activity in both age groups (Bannon, Curzon, Gunther & Decker, 1996). However, only aged rats showed 192 IgG-Saporin in Neonatal Rats 12 subsequent performance deficits in the Momk water rnaze, presumably due to a pre-existing hypofunction in the aged septohippocampal systern.…”

Section: E~pct-in~ Rilially H ~H C E J Cholïm~-gic D E F I C ï~-Ex-cmentioning

confidence: 90%

“…(Eierger-Sweeney et al, 1994;Leanza et al, 1995;Walsh et al, 1995). In contrast, unimpaired spatial navigation using the same injection methods has also been reported (Bannon et al, 1996;Baxter, Bucci, Sobel, Williams, Goman & Gallagher, 1996;Dornan et al, 1996). One explanation for these disparate results could be that ChAT activity in the basal forebrain must be reduced below a certain threshold before manifestation ofbehavioral deficits occurs.…”

Section: Eic' I W T D P H Mnzementioning

confidence: 95%

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Neural and behavioral effects of intracranial 192 IgG-saporin in neonatal rats: sexually dimorphic effects?

Sherren

Pappas

Fortin

1999

Developmental Brain Research

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Section: E~pct-in~ Rilially H ~H C E J Cholïm~-gic D E F I C ï~-Ex-cmentioning

confidence: 90%

Section: Eic' I W T D P H Mnzementioning

confidence: 95%

Neural and behavioral effects of intracranial 192 IgG-saporin in neonatal rats: sexually dimorphic effects?

Sherren

Pappas

Fortin

1999

Developmental Brain Research

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“…Some of the deficits resulting from combined EC and cholinergic lesions were found in tasks in which the cholinergic lesions alone had no significant effect (reference memory in the water maze, working memory in the radial maze, performance in the Hebb-Williams maze; see Table 4), suggesting that the increased amplitude of the deficits induced by combined lesions cannot be reduced to a simple addition of the specific effects of each lesion. Interestingly, Bannon et al (1996) found that whereas intraseptal injections of 192 IgG-saporin induced similar depletions of ChAT activity in the hippocampus of mature and aged rats, aged rats with lesions exhibited poor performance. It is not to exclude that this higher sensitivity to cholinergic lesions in aged rats might also be related to an interaction between the cholinergic lesion and existing damage in other structures.…”

Section: The Combination Of Ec and 192 Igg-saporin Lesionsmentioning

confidence: 96%

Combined Damage to Entorhinal Cortex and Cholinergic Basal Forebrain Neurons, Two Early Neurodegenerative Features Accompanying Alzheimer's Disease: Effects on Locomotor Activity and Memory Functions in Rats

Traissard

Herbeaux

Cosquer

et al. 2006

Neuropsychopharmacol

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In Alzheimer's disease (AD), cognitive decline is linked to cholinergic dysfunctions in the basal forebrain (BF), although the earliest neuronal damage is described in the entorhinal cortex (EC). In rats, selective cholinergic BF lesions or fiber-sparing EC lesions may induce memory deficits, but most often of weak magnitude. This study investigated, in adult rats, the effects on activity and memory of both lesions, alone or in combination, using 192 IgG-saporin (OX7-saporin as a control) and L-N-methyl-D-aspartate to destroy BF and EC neurons, respectively. Rats were tested for locomotor activity in their home cage and for working-and/or reference-memory in various tasks (water maze, Hebb-Williams maze, radial maze). Only rats with combined lesions showed diurnal and nocturnal hyperactivity. EC lesions impaired working memory and induced anterograde memory deficits in almost all tasks. Lesions of BF cholinergic neurons induced more limited deficits: reference memory was impaired in the probe trial of the water-maze task and in the radial maze. When both lesions were combined, performance never improved in the water maze and the number of errors in the Hebb-Williams and the radial mazes was always larger than in any other group. These results (i) indicate synergistic implications of BF and EC in memory function, (ii) suggest that combined BF cholinergic and fiber-sparing EC lesions may model aspects of anterograde memory deficits and restlessness as seen in AD, (iii) challenge the cholinergic hypothesis of cognitive dysfunctions in AD, and (iv) contribute to open theoretical views on AD-related memory dysfunctions going beyond the latter hypothesis.

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“…Several groups have reported that discrete lesions of either the MS/VDB or the NB, as well as a complete lesion of the entire basal forebrain (CBF) did not impair spatial working memory as tested in standard versions of the water maze and radial arm maze tasks (Baxter et al, 1995;Pizzo et al, 2002;Vuckovich et al, 2004;Frielingsdorf et al, 2006). However, other groups using more challenging, or more sensitive tasks of spatial memory have reported deficits following MS/VDB lesions (Baxter et al, 1995;Bannon et al, 1996;Chang and Gold, 2004), NB lesions (Baxter et al, 1995) or CBF lesions (Pizzo et al, 2002;Frick et al, 2004). A prediction arising from these studies was that the cholinergic system may play a role in strategy selection, rather than spatial memory per se.…”

Section: -Igg Saporin Lesionsmentioning

confidence: 99%

Revisiting the cholinergic hypothesis in the development of Alzheimer's disease

Craig

Hong

McDonald

2011

Neuroscience & Biobehavioral Reviews

389

263

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Alzheimer's disease (AD) is the most common form of dementia affecting the elderly population today; however, there is currently no accurate description of the etiology of this devastating disorder. No single factor theory has been demonstrated as being causative; however, an alternative theory suggests that the interaction of multiple risk factors is responsible for AD. In this thesis I present data suggesting a neuroprotective role for acetylcholine during aging. Using a rat model of cholinergic depletion of the medial septum, I explored the effects of four common risk factors for AD (stress, seizures, stroke and circadian dysfunction) targeted at the hippocampus and examined the effects on measures of hippocampal dependent (water maze) and hippocampal independent (fear conditioning) memory. Here, I propose a role for acetylcholinemediated compensatory mechanisms in the functional recovery observed following sub threshold insults similar to those commonly observed in the elderly. m First and foremost I would like to thank my supervisor, Dr. Robert McDonald. Thank you for your support and encouragement, and for allowing me the independence to pursue my own projects and to learn from my experiences. I would also like to thank Nhung Hong for doing the majority of the cholinergic depletion and stroke surgeries used in this thesis and for teaching me the techniques. I would like to give a huge thank you to Joelle Kopp who was an invaluable asset who assisted in histology and spent many hours on the microscope counting cholinergic neurons. I would also like to thank Dr. Christine Werk for patiently reading and re-reading early drafts of my papers and this thesis and Dr.Hugo Lehmann for all his help in running statistics and interpreting my data. In addition, many thanks go out to Courtney Lamb, Robert Court and Arthur Verhoef for helping me with histology and behavioural testing, to Keri Colwell for running the corticosterone assays, the Metz lab for kindly allowing me the use of their microscope and to Karen Dow-Cazel and the rest of the animal care staff for taking care of my rats and helping me set up the circadian experiements. The cholinergic hypothesis of Alzheimer's disease 6 Acknowledgements IVThe basal forebrain cholinergic system 8The role of the basal forebrain cholinergic system in learning and memory 10The current state of the cholinergic hypothesis of Alzhimer's disease 15Risk factors for Alzheimer's disease 16Multiple combinations of co-factors theory of Alzheimer's disease 22The role of the hippocampus in learning and memory 24Objective of the present thesis 25Chapter 2: A series of pilot studies to determine the threshold for behaviourally sub threshold stroke, seizures and stress 28Abstract 29Experiment la: The effect of a chronic variable stress schedule on performance of the water maze task 32 Materials and Methods 33Results 36Experiment lb: The effects of a variable restraint stress procedure on levels of blood borne CORT 39 Materials and Methods 39Results 40Discussion 41Experiment 2: T...

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Effects of intraseptal injection of 192-IgG-saporin in mature and aged Long-Evans rats

Cited by 39 publications

References 54 publications

Neural and behavioral effects of intracranial 192 IgG-saporin in neonatal rats: sexually dimorphic effects?

Neural and behavioral effects of intracranial 192 IgG-saporin in neonatal rats: sexually dimorphic effects?

Combined Damage to Entorhinal Cortex and Cholinergic Basal Forebrain Neurons, Two Early Neurodegenerative Features Accompanying Alzheimer's Disease: Effects on Locomotor Activity and Memory Functions in Rats

Revisiting the cholinergic hypothesis in the development of Alzheimer's disease

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