Effects of Intraperitoneal Thymoquinone on Chronic Neuropathic Pain in Rats

Abstract

In this study, we evaluated the protective effects of thymoquinone, the major constituent of Nigella sativa seeds on the neuropathic pain of rats with chronic constrictive injury of the sciatic nerve. Rats received repeated administration of thymoquinone (1.25, 2.5, and 5 mg/kg, i. p.) once a day for 14 days, beginning immediately after the nerve injury. Mechanical allodynia, cold allodynia, and thermal hyperalgesia were assessed with the von Frey filament, acetone drop, or radiant heat stimulus, respectively. Recent evidence points towards a role of oxidative stress, spinal glia activation, and cell death in the pathogenesis of neuropathic pain. Ionized calcium-binding adapter molecule 1 (a marker of microglia), glial fibrillary acidic protein (a marker of astroglia), Bcl2-associated X protein (a proapoptotic protein), and B-cell lymphoma protein 2 (an antiapoptotic protein) were measured using Western blot on days 3, 7, and 14 post chronic constrictive injury. The changes in the protein levels of malondialdehyde and glutathione, biomarkers of oxidative stress, were assessed by spectrophotometric assay on day 14 post chronic constrictive injury. Repeated treatment with thymoquinone (2.5 and 5 mg/kg) significantly alleviated behavioral signs of neuropathic pain. In the lumbar spinal cord of neuropathic rats, ionized calcium-binding adapter molecule 1 and Bcl2-associated X protein increased on day 3 post chronic constrictive injury, whereas B-cell lymphoma protein 2 did not significantly change. After repeated thymoquinone administration, the elevated Bcl2-associated X protein and ionized calcium-binding adapter molecule reduced on day 3, while the level of B-cell lymphoma protein 2 was even stimulated. Ionized calcium-binding adapter molecule and Bcl2-associated X protein/B-cell lymphoma protein 2 ratio declined by days 7 and 14; consequently, there were no significant differences among groups. No or little change was observed in the glial fibrillary acidic protein content during the study. Chronic constrictive injury produced a significant increase in the levels of malondialdehyde and decrease in the contents of glutathione on day 14. Thymoquinone treatment (2.5 and 5 mg/kg) restored the levels of malondialdehyde. High dose of thymoquinone (5 mg/kg) also reversed the decreased glutathione in the injured animals. Our results indicate that, microglia, apoptotic factors, and oxidative stress rather than astroglia contribute to the pathogenesis of chronic constrictive injury, and thymoquinone plays an anti-nociceptive role possibly by antioxidant effects and inhibition of microglia activity.

Review on Clinical Trials of Black Seed (Nigella sativa ) and Its Active Constituent, Thymoquinone

“…Cell culture studies and animal models have indicated several therapeutic potentials, such as anti-cancer [5, 7–9], antimicrobial [10, 11], analgesic [12, 13], antipyretic [14], contraceptive and anti-fertility, anti-oxytocic [3], anti-tussive [15], anti-inflammatory [12, 16], and anti-oxidant [17–19] potentials, for black seed and its active component TQ. NS or TQ anticancer activity has been demonstrated for blood, breast, colon, pancreatic, liver, lung, fibrosarcoma, prostate, and cervix cancer cell lines and in animal models of lung, kidney, skin, colon, and breast cancer [7].…”

The acute effects of thymoquinone on acute peripheral nerve injury: an experimental study

“…[33][34][35][36][37] Effects of TQ on the peripheral nervous system were reported in 3 studies. [10,11] The first of these was performed in an experimental streptozotocin-induced diabetic neuropathy model. The authors reported that TQ improved ultrastructural features of axons remarkably.…”

“…[10] In the second study, Amin et al used a rat model of chronic neuropathy and concluded that TQ plays an anti-nociceptive role, possibly by exerting antioxidant effects and inhibiting microglial activity. [11] However, no published studies have assessed the acute effects of TQ in a peripheral nerve model. Current histological and stereological results suggest that TQ has no therapeutic effect on acute nerve injury.…”

“…[6][7][8][9] However, studies analyzing the effects of TQ on the peripheral nervous system have focused only on models of neuropathic pain. [10,11] To the best of our knowledge, no studies have assessed therapeutic effects of TQ on peripheral nerves following an acute crush injury.…”

Oxidation of Monoterpenes Catalysed by a Water‐Soluble MnIII PEG‐Porphyrin in a Biphasic Medium

“…[1][2][3][4][5][6][7] However,T Qc an be obtained in large quantities through the oxidation of carvacrol and thymol( Scheme 1), which are abundantly available in oregano essential oils. [1][2][3][4][5][6][7] However,T Qc an be obtained in large quantities through the oxidation of carvacrol and thymol( Scheme 1), which are abundantly available in oregano essential oils.…”