Effects of intraduodenal injection of Lactobacillus johnsonii La1 on renal sympathetic nerve activity and blood pressure in urethane-anesthetized rats

Tanida, Mamoru; Yamano, Toshìo; Maeda, Keisuke; Okumura, Nobuaki; Fukushima, Yoichi; Nonomura, Katsuya

doi:10.1016/j.neulet.2005.07.036

Cited by 134 publications

(127 citation statements)

References 10 publications

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“…These earlier experiments involving dorsal root fiber (18) and vagal nerve recording (42) do not prove that in our experiments the probiotic acted on neurons without first acting on the immune system. Ingestion of the probiotic for 9 days may have altered the immune system in such a way that the neural response to CRD was altered.…”

Section: Discussioncontrasting

confidence: 95%

“…The present report may be consistent with previous evidence that commensal bacteria can signal to sensory neurons and reset their excitability state (18,42), a state that can persist even after the bacteria are no longer present. These earlier experiments involving dorsal root fiber (18) and vagal nerve recording (42) do not prove that in our experiments the probiotic acted on neurons without first acting on the immune system.…”

Section: Discussionsupporting

confidence: 93%

“…The present work extends previous observations that ingestion of probiotic bacteria can moderate pain transmission and neurally based reflexes in the absence of an overt inflammatory model (18,25,32,42). CRD in healthy animals, and in the apparent absence of inflammation, increased the excitability of DRG neurons that were isolated and recovered in culture hours afterward.…”

Section: Discussionsupporting

confidence: 89%

“…Lactobacillus reuteri (L. reuteri) ingestion can alter the excitability of myenteric intrinsic sensory neurons that were recorded from colon segments taken from healthy rats. Remarkably, in anesthetized healthy rats, intraduodenal injection of a Lactobacillus species alters spike discharge activity in cervical vagal and renal nerve trunks (42) within 20 -30 min. This might also be relevant for our experiments since nodose neurons innervate the murine distal colon (2,47).…”

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confidence: 99%

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Lactobacillus reuteri ingestion prevents hyperexcitability of colonic DRG neurons induced by noxious stimuli

Ma¹,

Mao²,

Wang³

et al. 2009

American Journal of Physiology-Gastrointestinal and Liver Physi

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Kunze W. Lactobacillus reuteri ingestion prevents hyperexcitability of colonic DRG neurons induced by noxious stimuli. Am J Physiol Gastrointest Liver Physiol 296: G868 -G875, 2009. First published January 29, 2009 doi:10.1152/ajpgi.90511.2008.-Lactobacillus species ingestion can decrease autonomic responses and spinal fiber discharge to nociceptive colorectal distension (CRD), even in the absence of inflammation. The present study aimed to determine whether dorsal root ganglion (DRG) somas could be a locus where the antinociceptive probiotic may have an effect. Healthy rats were fed with Lactobacillus reuteri or vehicle control for 9 days whereupon they were anesthetized, and intermittent distal colonic CRD at 80 mmHg distension was either performed for 1 h or not. The animals were immediately euthanized and patch-clamp recordings taken after isolation and overnight culture from those DRG that projected to the distal colon. CRD decreased the threshold for action potential generation and increased the number of spikes discharged during a standard depolarizing test stimulus, and this effect was blocked by prior probiotic ingestion. The increase in excitability was paralleled by an increase in DRG capacitance, which was not altered by Lactobacillus reuteri ingestion. CRD did not increase tissue weight or myeloperoxidase activity. We suggest that the effects of CRD may have been caused by activity-dependent neurotransmission between DRG somas. CRD evoked increases in action potential upstroke speed, which suggests that it may also have led to augmentation of sodium channel conductances. Probiotic ingestion may have interfered with this hypothetical mechanism since it blocked the effect of CRD on the action potential. colorectal distension; dorsal root ganglion; probiotics; sensory neurons PROBIOTICS ARE HEALTH-PROMOTING commensal bacteria that can have therapeutic or preventative roles when ingested. Probiotics are most frequently of the Lactobacillus or Bifidobacterium species, and clinically beneficial effects of probiotics have been described in travelers' diarrhea, irritable bowel syndrome, and inflammatory bowel disease (13,29,35,44,46). In addition, probiotics can modulate postinflammatory gut hypersensitivity and alter neuropeptide content (20,43). Because of their therapeutic potential and their presumed consumer safety, the actions of probiotics on the physiological mechanisms underlying peripheral and centrally directed nociceptive signals are increasingly being investigated.Ingestion of probiotic commensals can decrease colorectal distension (CRD)-evoked pain in healthy animals, that is, in the absence of overt inflammation in the intestine. Experimental modulation of intestinal motility (32) and visceral pain (18,32,43) by Lactobacillus species have been convincingly demonstrated for the murine colon. Lactobacillus reuteri (L. reuteri) ingestion can alter the excitability of myenteric intrinsic sensory neurons that were recorded from colon segments taken from healthy rats. Remarkably, in anesthetized he...

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Section: Discussioncontrasting

confidence: 95%

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 89%

mentioning

confidence: 99%

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Lactobacillus reuteri ingestion prevents hyperexcitability of colonic DRG neurons induced by noxious stimuli

Ma¹,

Mao²,

Wang³

et al. 2009

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…GF animals are born in aseptic conditions, which may include removal from the mother by Cesarean section and immediate transfer of the newborn to an isolator, where all incoming air, food, and water are sterilized. There is a wide range of differences in brain (and gut) biochemistry (39,57); hypothalamic/pituitary/ adrenal (HPA) axis responses (37); and affective (38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48), social (48,(58)(59)(60), metabolic function, and ingestive behaviors (54)(55)(56) between GF animals and control animals that have normal or pathogen-free flora and were reared by normally colonized mothers (39,40). Thus, observed brain and behavioral changes could be mediated by the lack of gut microbiota directly or indirectly through one or several of the non-brain-related alterations.…”

Section: Gut Microbiota Effects On the Brain: Preclinical Evidencementioning

confidence: 99%