Effects of Intra-Articular Resveratrol Injections on Cartilage Destruction and Synovial Inflammation in Experimental Temporomandibular Joint Osteoarthritis

Yuce, Pinar; Hoşgör, Hatice; Rençber, Selenay Furat; Yazır, Yusufhan

doi:10.1016/j.joms.2020.09.015

Cited by 15 publications

(19 citation statements)

References 34 publications

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“…The mice were divided into the normal control group (NC), OA group (OA), and RES group (RES). The TMJOA model was set up by injection of collagenase (12.5 μ l, Sigma Biochemical, St. Louis) in the upper cavity of TMJ according to previous studies and made some modifications [ 2 , 18 ]. 1 week later, the OA mice were injected by RES (100 μ g/10 μ l) [ 18 ] 3 times one week.…”

Section: Methodsmentioning

confidence: 99%

“…24 h later, the cells were harvested and used for western blot analysis. 3 BioMed Research International collagenase (12.5 μl, Sigma Biochemical, St. Louis) in the upper cavity of TMJ according to previous studies and made some modifications [2,18]. 1 week later, the OA mice were injected by RES (100 μg/10 μl) [18] 3 times one week.…”

Section: Il-1β-induced Apoptosismentioning

confidence: 99%

“…Some studies have demonstrated that RES alleviates rheumatoid arthritis by reducing inflammation, inhibiting MAPK signaling pathways, and suppressing angiogenesis [ 17 ]. Although some studies revealed that the intra-articular RES treatment could exert a curative effect by preventing the inflammation and cartilage destruction of TMJOA [ 18 ], the mechanism remained unknown.…”

Section: Introductionmentioning

confidence: 99%

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The Antioxidant Resveratrol Protects against Chondrocyte Apoptosis by Regulating the COX-2/NF-κB Pathway in Created Temporomandibular Osteoarthritis

Chen

et al. 2021

BioMed Research International

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Temporomandibular joint osteoarthritis (TMJOA) is characterized by chronic inflammatory degradation of mandibular condylar cartilage (MCC). Studies have found a positive correlation between inflammation and cyclooxygenase- (COX-) 2 in OA pathology. NF-κB is a crucial transcription factor of inflammatory and immune responses in the cause of TMJOA pathology. Resveratrol (RES) plays a critical role in antioxidation and anti-inflammation. But, studies on the effects of RES on TMJOA are very limited. So, the purpose of this study is to investigate the antioxidant and protective effects of RES against MCC degradation through downregulating COX-2/NF-κB expression. In vitro studies, the MCC cells were divided into three groups: the NC group, OA group, and RES group. The optimum dose of RES (10 μM) was determined. The TMJOA model of mice was created by injection of collagenase. And mice were injected with RES (100 μg/10 μl) 3 times one week for 4 weeks in the RES group. The expressions of COX-2, P65, MMP1, MMP13, COL2, and ACAN were measured by RT-PCR. Morphological changes of MCC were studied with HE staining. The results showed that inflammation could induce MCC degradation in vitro and vivo, while RES could reverse the degradation. Meanwhile, RES could downregulate COX-2/NF-κB/MMP expression and increase cartilage markers in vitro and vivo studies. The results indicated that RES treatment had antioxidant effects against chondrocyte apoptosis by downregulating the COX-2/NF-κB pathway in created TMJOA.

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Section: Methodsmentioning

confidence: 99%

Section: Il-1β-induced Apoptosismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Antioxidant Resveratrol Protects against Chondrocyte Apoptosis by Regulating the COX-2/NF-κB Pathway in Created Temporomandibular Osteoarthritis

Chen

et al. 2021

BioMed Research International

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“…Only the in vitro mechanism was determined, which is a limitation of this study. Importantly, intra-articular injection of resveratrol exerted a curative effect by preventing inflammation and cartilage destruction [ 96 ].…”

Section: Candidate Therapeutic Agentsmentioning

confidence: 99%

Therapeutic Single Compounds for Osteoarthritis Treatment

et al. 2021

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Osteoarthritis (OA) is an age-related degenerative disease for which an effective disease-modifying therapy is not available. Natural compounds derived from plants have been traditionally used in the clinic to treat OA. Over the years, many studies have explored the treatment of OA using natural extracts. Although various active natural extracts with broad application prospects have been discovered, single compounds are more important for clinical trials than total natural extracts. Moreover, although natural extracts exhibit minimal safety issues, the cytotoxicity and function of all single compounds in a total extract remain unclear. Therefore, understanding single compounds with the ability to inhibit catabolic factor expression is essential for developing therapeutic agents for OA. This review describes effective single compounds recently obtained from natural extracts and the possibility of developing therapeutic agents against OA using these compounds.

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“…The sterile 30-gauge needle was positioned on the posteroinferior edge of the zygomatic arch (the roof of the joint fossa) and passed through the posterolateral edge of the mandibular condyle toward the antero-medial direction to reach the superior joint cavity. (30) OA was induced in the animals TMJs on day 0 by a unilateral right side single intra-articular injection of 50 μl of Complete Freund's adjuvant (CFA) (5881; Sigma-Aldrich, USA), diluted 1:1(oil: saline). (31)(32)(33) The animals received 300 mg/ kg Acetaminophen (Paracetamol) added in drinking water after OA induction to decrease pain.…”

Section: Induction Of Tmj-oamentioning

confidence: 99%

Biochemical and Histological Evaluation of different intra-articular injections as therapy for Temporomandibular Joint Osteoarthritis in Rats

Arafat

Kamel

2021

Egyptian Dental Journal

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Introduction: Temporomandibular joint (TMJ) osteoarthritis is usually treated by intraarticular injection of medications. Intra-articular hyaluronic acid (HA) injection reduces friction and stress on the joint cartilage through lubrication, chondro-protection and proteoglycans synthesis. Platelet Rich Plasma (PRP) with its high concentration of growth factors plays a great role in joint repair through enhancing osteo-inductivity, and chondrogenic differentiation. This study aims at comparing intra-articular injection of combination of hyaluronic acid and platelet rich plasma to intra-articular injection of each of them alone.Materials and Methods: 24 young adult male 250-300 grams albino rats were used in the current study divided randomly into 4 groups: Group 1 (control) in which animals were left without treatment. Group 2 which was subdivided into 3 study groups, in Group 2A: the animals received 2 doses of HA, in Group 2B: the animals received 2 doses of PRP, and in Group 2C: the animals received 2 doses of combined PRP and HA. After 28 days, the animals were sacrificed and histological analysis was performed. Synovial fluid Samples were extracted before each intraarticular injection, the concentration of vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2) were detected and statistically analyzed.Results: Group 2C showed superior findings to the other 2 study groups regarding fibrocartilage and bone regeneration, while Group 2B was superior to group 2A regarding the study parameters. Conclusion:Intra-articular injection of combination of PRP and HA is superior to intraarticular injection of HA or PRP alone for the treatment of TMJ osteoarthritis in rats.

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Effects of Intra-Articular Resveratrol Injections on Cartilage Destruction and Synovial Inflammation in Experimental Temporomandibular Joint Osteoarthritis

Cited by 15 publications

References 34 publications

The Antioxidant Resveratrol Protects against Chondrocyte Apoptosis by Regulating the COX-2/NF-κB Pathway in Created Temporomandibular Osteoarthritis

The Antioxidant Resveratrol Protects against Chondrocyte Apoptosis by Regulating the COX-2/NF-κB Pathway in Created Temporomandibular Osteoarthritis

Therapeutic Single Compounds for Osteoarthritis Treatment

Biochemical and Histological Evaluation of different intra-articular injections as therapy for Temporomandibular Joint Osteoarthritis in Rats

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