Effects of intestinal fatty acid-binding protein overexpression on fatty acid metabolism in Caco-2 cells

Darimont, Christian; Gradoux, Nathalie; Persohn, Elke; Cumin, Frédéric; Pover, Alain De

doi:10.1016/s0022-2275(20)32077-0

Cited by 45 publications

(7 citation statements)

References 31 publications

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“…Many studies have generally focused on the role of the intestinal FABP2 in the absorption, transport, and metabolism of saturated and unsaturated long-chain fatty acids [ 7 , 8 , 9 ]. Darimont et al, (2000) showed that a high level of FABP2 expressed in a differentiated enterocyte model inhibits incorporation of fatty acid, but the mechanism is still unknown [ 10 ]. Additionally, animal model experiments revealed that Fabp2 null ( Fabpi-/- ) mice show changes in body weight and are hyperinsulinemic.…”

Section: Discussionmentioning

confidence: 99%

“…This protein participates in the absorption, intracellular transport, and metabolism of dietary fatty acids and their acyl-CoA esters in small intestine [ 7 , 8 , 9 ]. There is experimental evidence that high levels of FABP2, expressed in a differentiated enterocyte model, may inhibit fatty acid incorporation by a currently-undefined mechanism [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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FABP2 Ala54Thr Polymorphism and Post-Training Changes of Body Composition and Biochemical Parameters in Caucasian Women

Leońska-Duniec

Świtała

Ahmetov

et al. 2021

Genes

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The functional FABP2 Ala54Thr polymorphism (rs1799883) is strongly associated with lipid and carbohydrate metabolism, although the function of its potential modifying effect on training-induced changes in obesity-related parameters is still unknown. The aim of the present study was to investigate the influence of the Ala54Thr polymorphism on post-training changes of selected body mass and body composition measurements, as well as with biochemical parameters of energy metabolism. Accordingly, alleles and genotypes distribution in a group of 168 young, nonobese Caucasian women measured for chosen body composition parameters, lipid profile, and glucose levels before and after the completion of a 12-week aerobic training program were studied. Although the obtained results showed changes in body mass, BMI, FM, %FM, FFM, TBW, HDL-C, and glucose levels during the training program, none of the examined parameters changed significantly across the FABP2 genotypes. Instead, we found a main effect of genotype on BMI (p = 0.033), with carriers of the Thr54 allele having a higher BMI during the whole study period compared with the Ala54 carriers. We confirm that the FABP2 Ala54Thr polymorphism may help identify women at risk for overweight and obesity. However, we did not notice evidence of an interaction between physical activity and the Ala54Thr polymorphism on the examined parameters.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

FABP2 Ala54Thr Polymorphism and Post-Training Changes of Body Composition and Biochemical Parameters in Caucasian Women

Leońska-Duniec

Świtała

Ahmetov

et al. 2021

Genes

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“…Further support for this hypothesis is derived from the observation that compounds which compete for FABP binding impair the cytoplasmic diffusion of NBD-stearate (13) and from the enhanced rates of NBD-stearate diffusion in L cell fibroblasts (14,15) and mouse embryonic stem cells (12) transfected with L-FABP, intestinal FABP (I-FABP), or sterol carrier protein 2. On the other hand, Darimont et al (16) found that overexpression of I-FABP in stably transfected Caco-2 cells inhibited cellular incorporation of radiolabeled palmitate.…”

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confidence: 99%

Kinetic Model of Protein-Mediated Ligand Transport: Influence of Soluble Binding Proteins on the Intermembrane Diffusion of a Fluorescent Fatty Acid

Zucker

2001

Biochemistry

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The mechanism (or mechanisms) whereby fatty acids and other amphipathic compounds are transported from the plasma membrane to intracellular sites of biotransformation remains poorly defined. In an attempt to better characterize the role of cytosolic binding proteins in this process, a kinetic model of intermembrane ligand transport was developed in which diffusional transfer of ligand between membrane and protein is assumed. The model was tested by utilizing stopped-flow techniques to monitor the transfer of the fluorescent fatty acid analogue, 12-anthroyloxy stearate (12-AS), between model membrane vesicles. Studies were conducted in the presence or absence of bovine serum albumin (BSA), liver fatty acid-binding protein (L-FABP), and intestinal fatty acid-binding protein (I-FABP) in order to determine the effect of soluble proteins on the rate of intermembrane ligand transfer. As predicted by the model, the initial velocity of 12-AS arrival at the acceptor membrane increases in an asymptotic manner with the acceptor concentration. Furthermore, probe transfer velocity was found to decline asymptotically with increasing concentrations of BSA or L-FABP, proteins that exhibit diffusional transfer kinetics. This observation was found to hold true independent of whether donor or acceptor vesicles were preequilibrated with the protein. In contrast, 12-AS transfer velocity exhibited a linear correlation with the concentration of I-FABP, a protein that is thought to transport fatty acids, at least in part, via a collisional mechanism. Taken together, these findings validate the derived kinetic model of protein-mediated ligand transport and further suggest that the mechanism of ligand-protein interaction is a key determinant of the effect of cytosolic proteins on intracellular ligand diffusion.

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“…However, in L-cells expressing larger amounts of intestinal FABP, FA uptake decreased [36]. In a differentiated enterocyte model high levels of intestinal FABP also inhibited FA incorporation [37]. A direct correlation between L-FABP content and FA uptake was observed in HepG2 cells either transfected with antisense L-FABP mRNA or treated with a peroxisome proliferator [38].…”

Section: Discussionmentioning

confidence: 91%

Fatty-acid-binding proteins do not protect against induced cytotoxicity in a kidney cell model

Zimmerman¹,

Veerkamp²

2001

Biochemical Journal

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Intracellular accumulation of fatty acids (FAs) is a well-described consequence of renal ischaemia and may lead to lethal cell injury. Fatty-acid-binding proteins (FABPs) are small cytosolic proteins with high affinity for FAs. They may protect vital cellular functions by binding to and promoting the metabolism of FAs, thereby reducing their intracellular concentration. In this study we investigated the putative cytoprotective role of FABPs in a Madin–Darby canine kidney (MDCK) cell model for renal damage. We studied the effects of transfection with cDNA encoding heart FABP, adipocyte FABP or liver FABP on cytotoxicity induced by chemical anoxia or FAs. Transfection of MDCK type II cells with these cDNA types caused a 5–20-fold increase in FABP content, but did not change the rate or extent of palmitate uptake. After 1h of incubation with KCN, all cell types showed reduced viability and cellular ATP content and an intracellular accumulation of non-esterified FAs. High extracellular concentrations of oleate, but not palmitate, caused a markedly decreased cell viability and cellular ATP content. Oleate accumulated in non-esterified form in these cells. Simultaneous addition of glucose ameliorated the damaging effects of KCN or oleate, indicating that glycolytic ATP could substitute for uncoupled oxidative phosphorylation. No significant differences in the effects of chemical anoxia or oleate were observed between non-transfected, mock-transfected and FABP-cDNA-transfected cells. Non-esterified FA accumulation was not reduced in any of the FABP-cDNA-transfected cell lines. In conclusion, our data do not provide evidence for a cytoprotective role of FABP in this kidney cell model.

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Effects of intestinal fatty acid-binding protein overexpression on fatty acid metabolism in Caco-2 cells

Cited by 45 publications

References 31 publications

FABP2 Ala54Thr Polymorphism and Post-Training Changes of Body Composition and Biochemical Parameters in Caucasian Women

FABP2 Ala54Thr Polymorphism and Post-Training Changes of Body Composition and Biochemical Parameters in Caucasian Women

Kinetic Model of Protein-Mediated Ligand Transport: Influence of Soluble Binding Proteins on the Intermembrane Diffusion of a Fluorescent Fatty Acid

Fatty-acid-binding proteins do not protect against induced cytotoxicity in a kidney cell model

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