Effects of intermittent hydrostatic pressure and BMP‐2 on osteoarthritic human chondrocyte metabolism in vitro

Smith, Robert L.; Lindsey, Derek P.; Dhulipala, Lakshmi; Harris, Alex H. S.; Goodman, Stuart B.; Maloney, William J.

doi:10.1002/jor.21250

Cited by 23 publications

(18 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another study, both human normal and OA AC lacked BMP-7 protein expression, which was only detected in fetal, developing AC [96]. rBMP-2 induces anabolic gene expression, including ACAN and COL2A1 as well as increased proteoglycan deposition in human normal adult AC (both young and aged) cell cultures as well as OA AC cell cultures [91,98,99,100,101]. Expression of catabolic MMP-2 and MMP-3 mRNA is not affected by rBMP-2 [99].…”

Section: Additional Growth Factor Signaling Pathways In Human Adulmentioning

confidence: 99%

“…rBMP-2 induces anabolic gene expression, including ACAN and COL2A1 as well as increased proteoglycan deposition in human normal adult AC (both young and aged) cell cultures as well as OA AC cell cultures [91,98,99,100,101]. Expression of catabolic MMP-2 and MMP-3 mRNA is not affected by rBMP-2 [99]. Yet, another study in human OA AC monolayer cultures shows rBMP-2 induced catabolic MMP-9 , MMP-13 and ADAMTS-5 mRNA expression, which is at least partially mediated by WNT/β-catenin signaling [93].…”

Section: Additional Growth Factor Signaling Pathways In Human Adulmentioning

confidence: 99%

See 1 more Smart Citation

Onset and Progression of Human Osteoarthritis—Can Growth Factors, Inflammatory Cytokines, or Differential miRNA Expression Concomitantly Induce Proliferation, ECM Degradation, and Inflammation in Articular Cartilage?

Boehme,

Rolauffs

2018

IJMS

View full text Add to dashboard Cite

Osteoarthritis (OA) is a degenerative whole joint disease, for which no preventative or therapeutic biological interventions are available. This is likely due to the fact that OA pathogenesis includes several signaling pathways, whose interactions remain unclear, especially at disease onset. Early OA is characterized by three key events: a rarely considered early phase of proliferation of cartilage-resident cells, in contrast to well-established increased synthesis, and degradation of extracellular matrix components and inflammation, associated with OA progression. We focused on the question, which of these key events are regulated by growth factors, inflammatory cytokines, and/or miRNA abundance. Collectively, we elucidated a specific sequence of the OA key events that are described best as a very early phase of proliferation of human articular cartilage (AC) cells and concomitant anabolic/catabolic effects that are accompanied by incipient pro-inflammatory effects. Many of the reviewed factors appeared able to induce one or two key events. Only one factor, fibroblast growth factor 2 (FGF2), is capable of concomitantly inducing all key events. Moreover, AC cell proliferation cannot be induced and, in fact, is suppressed by inflammatory signaling, suggesting that inflammatory signaling cannot be the sole inductor of all early OA key events, especially at disease onset.

show abstract

Section: Additional Growth Factor Signaling Pathways In Human Adulmentioning

confidence: 99%

Section: Additional Growth Factor Signaling Pathways In Human Adulmentioning

confidence: 99%

Onset and Progression of Human Osteoarthritis—Can Growth Factors, Inflammatory Cytokines, or Differential miRNA Expression Concomitantly Induce Proliferation, ECM Degradation, and Inflammation in Articular Cartilage?

Boehme,

Rolauffs

2018

IJMS

View full text Add to dashboard Cite

show abstract

“…Macroscopically intact cartilage was dissected and the chondrocytes were dissociated from the matrix as described previously. 16 Chondrocytes were cultured in monolayer using Dulbecco's Modified Eagle's Medium (Hyclone, Thermo Scientific, Inc.) supplemented with 25 mg/mL ascorbate, 2 mM L-glutamine, antimicrobials (100 U/mL penicillin, 100 mg/mL streptomycin, and 0.25 mg/mL fungizone), and 10% FBS (Gibco, Invitrogen) for 24 h at 37°C.…”

Section: Chondrocyte Isolation and Culturementioning

confidence: 99%

Comparative Potential of Juvenile and Adult Human Articular Chondrocytes for Cartilage Tissue Formation in Three-Dimensional Biomimetic Hydrogels

Smeriglio

Lai

Dhulipala

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

Regeneration of human articular cartilage is inherently limited and extensive efforts have focused on engineering the cartilage tissue. Various cellular sources have been studied for cartilage tissue engineering including adult chondrocytes, and embryonic or adult stem cells. Juvenile chondrocytes (from donors below 13 years of age) have recently been reported to be a promising cell source for cartilage regeneration. Previous studies have compared the potential of adult and juvenile chondrocytes or adult and osteoarthritic (OA) chondrocytes. To comprehensively characterize the comparative potential of young, old, and diseased chondrocytes, here we examined cartilage formation by juvenile, adult, and OA chondrocytes in three-dimensional (3D) biomimetic hydrogels composed of poly(ethylene glycol) and chondroitin sulfate. All three human articular chondrocytes were encapsulated in the 3D biomimetic hydrogels and cultured for 3 or 6 weeks to allow maturation and extracellular matrix formation. Outcomes were analyzed using quantitative gene expression, immunofluorescence staining, biochemical assays, and mechanical testing. After 3 and 6 weeks, juvenile chondrocytes showed a greater upregulation of chondrogenic gene expression than adult chondrocytes, while OA chondrocytes showed a downregulation. Aggrecan and type II collagen deposition and glycosaminoglycan accumulation were high for juvenile and adult chondrocytes but not for OA chondrocytes. Similar trend was observed in the compressive moduli of the cartilage constructs generated by the three different chondrocytes. In conclusion, the juvenile, adult and OA chondrocytes showed differential responses in the 3D biomimetic hydrogels. The 3D culture model described here may also provide a useful tool to further study the molecular differences among chondrocytes from different stages, which can help elucidate the mechanisms for age-related decline in the intrinsic capacity for cartilage repair.

show abstract

“…Obtain cartilage from tissue discarded during total knee arthroplasty and dissect as described previously 8 .…”

Section: Articular Chondrocyte Isolationmentioning

confidence: 99%

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

Smeriglio

Lai

Yang

et al. 2015

JoVE

View full text Add to dashboard Cite

Human articular cartilage is highly susceptible to damage and has limited self-repair and regeneration potential. Cell-based strategies to engineer cartilage tissue offer a promising solution to repair articular cartilage. To select the optimal cell source for tissue repair, it is important to develop an appropriate culture platform to systematically examine the biological and biomechanical differences in the tissue-engineered cartilage by different cell sources. Here we applied a three-dimensional (3D) biomimetic hydrogel culture platform to systematically examine cartilage regeneration potential of juvenile, adult, and osteoarthritic (OA) chondrocytes. The 3D biomimetic hydrogel consisted of synthetic component poly(ethylene glycol) and bioactive component chondroitin sulfate, which provides a physiologically relevant microenvironment for in vitro culture of chondrocytes. In addition, the scaffold may be potentially used for cell delivery for cartilage repair in vivo. Cartilage tissue engineered in the scaffold can be evaluated using quantitative gene expression, immunofluorescence staining, biochemical assays, and mechanical testing. Utilizing these outcomes, we were able to characterize the differential regenerative potential of chondrocytes of varying age, both at the gene expression level and in the biochemical and biomechanical properties of the engineered cartilage tissue. The 3D culture model could be applied to investigate the molecular and functional differences among chondrocytes and progenitor cells from different stages of normal or aberrant development.

show abstract

Effects of intermittent hydrostatic pressure and BMP‐2 on osteoarthritic human chondrocyte metabolism in vitro

Cited by 23 publications

References 51 publications

Onset and Progression of Human Osteoarthritis—Can Growth Factors, Inflammatory Cytokines, or Differential miRNA Expression Concomitantly Induce Proliferation, ECM Degradation, and Inflammation in Articular Cartilage?

Onset and Progression of Human Osteoarthritis—Can Growth Factors, Inflammatory Cytokines, or Differential miRNA Expression Concomitantly Induce Proliferation, ECM Degradation, and Inflammation in Articular Cartilage?

Comparative Potential of Juvenile and Adult Human Articular Chondrocytes for Cartilage Tissue Formation in Three-Dimensional Biomimetic Hydrogels

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

Contact Info

Product

Resources

About