Effects of Interleukin‐6 on Proliferation and Proteoglycan Metabolism in Articular Chondrocyte Cultures

Jikko, Akitoshi; Wakisaka, Takashi; Iwamoto, Masahiro; Hiranuma, Hiroko; Kato, Yukio; Maeda, Takashi; Fujishita, Masami; Fuchihata, Hajime

doi:10.1006/cbir.1998.0304

Cited by 54 publications

(40 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chondrocyte cloning is formed by cell proliferation and enhanced production of extracellular matrix without proper rearrangement of chondrocytes in the damaged cartilage (3), although the precise molecular mechanism is unknown. Growth factors including TGF␤ are suggested to play a role in chondrocyte cloning according to data from in vitro cloning assays (34,35). In the present study, we demonstrated that TGF␤-induced chondrocyte proliferation and chondrocyte cloning in agarose gel culture, which was stimulated by treatment with a mixture of TGF␤ and IGF-1, are abrogated by treatment with ADAM inhibitor, neutralizing anti-ADAM-12 antibody, or siRNA for ADAM-12.…”

Section: Discussionsupporting

confidence: 57%

ADAM‐12 (meltrin α) is involved in chondrocyte proliferation via cleavage of insulin‐like growth factor binding protein 5 in osteoarthritic cartilage

Okada¹,

Mochizuki²,

Yatabe³

et al. 2008

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. ADAMs are a gene family of multifunctional proteins. We undertook this study to determine which ADAM species is up-regulated in osteoarthritic (OA) cartilage and to examine its pathobiologic function.Methods. Expression of the 13 different metalloproteinase-type ADAMs was screened by reverse transcription-polymerase chain reaction (PCR), and expression levels of prototype membrane-anchored ADAM-12 (ADAM-12m) were determined by real-time PCR. ADAM-12m expression in articular cartilage was examined by in situ hybridization, immunohistochemistry, and immunoblotting. Chondrocytes were used for functional analyses of ADAM-12m.Results. ADAM-12m was selectively expressed in 87% of OA cartilage, and the expression level was significantly higher in OA cartilage than in normal cartilage. In situ hybridization showed that OA chondrocytes were responsible for the expression. Osteoarthritis (OA) is characterized by the breakdown of articular cartilage, leading to pain and loss of joint function. Articular cartilage is a narrow layer of specialized extracellular matrix, consisting primarily of collagens and proteoglycans, that is elaborated and maintained by a small number of articular chondrocytes. In normal cartilage, chondrocytes sustain a balance between the synthesis and the degradation of matrix components, resulting in stability of the tissue over time. This equilibrium is disturbed in degenerative joint diseases such as OA (1). Chondrocyte cloning, also known as chondrocyte cluster formation, is a major characteristic phenotype of OA cartilage (2), and it could reflect

show abstract

Section: Discussionsupporting

confidence: 57%

ADAM‐12 (meltrin α) is involved in chondrocyte proliferation via cleavage of insulin‐like growth factor binding protein 5 in osteoarthritic cartilage

Okada¹,

Mochizuki²,

Yatabe³

et al. 2008

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…Among these cytokines, IL-6 was detected in patients with OA as well as in patients with rheumatoid arthritis 22) . Pallotta et al 23) demonstrated that laser irradiation was effective in significantly reducing the total number of leukocytes as well as neutrophils in joint cavities.…”

Section: Discussionmentioning

confidence: 99%

“…Pallotta et al 23) demonstrated that laser irradiation was effective in significantly reducing the total number of leukocytes as well as neutrophils in joint cavities. Cartilage extracellular matrix degradation and suppression of extracellular matrix biosynthesis are regulated by a significant increase in the levels of cytokines in the affected synovial joint 22) . On the other hand, a critical function of IL-10 is to limit inflammatory responses 24) .…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammation Effects of Low Intensity Laser Therapy on Monosodium Iodoacetate-induced Osteoarthritis in Rats

Kim

2013

J Phys Ther Sci

View full text Add to dashboard Cite

Abstract.[Purpose] The purpose of this study was to investigate the effects of low intensity laser therapy (LILT) on inflammatory osteoarthritis in the knee joint of rats.[Subjects] Thirty Sprague-Dawley rats were randomly divided into 3 groups with 10 rats each: the normal, control, and LILT groups. The arthritis group was treated with LILT using a gallium-aluminum-arsenide diode laser over during 3 weeks. All treatments were applied once a day, 5 days per week for 21 days. The inflammation cytokine and the articular index were used to assess the arthritic symptoms. The levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in the serum were used as outcome measures.[Results] The arthritis index points of rats in the LILT group were lower than that of the control group. Similarly, TNF-α, IL-1β, and IL-6 concentrations in serum were significantly decreased in the LILT group compared to the control group, which coincided with behavioral studies.[Conclusion] This study shows that LILT is capable of reducing inflammatory cytokine concentrations induced by monosodium iodoacetate osteoarthritis in rats.

show abstract

“…A significant elevation in the expression of IL-6 mRNA has been observed in OA-affected cartilage, and the IL-6 levels in serum and synovial fluid have been documented to be increased among OA patients (Kaneko et al, 2000). Moreover, human recombinant IL-6 has been demonstrated to enhance human recombinant IL-1β-induced proteoglycan degradation and to inhibit chondrocyte proliferation (Jikko et al, 1998). Known variations within the IL-6 gene have been repeatedly screened in a number of association studies.…”

Section: Discussionmentioning

confidence: 99%

Association of the IL-6 -174G/C gene polymorphism with knee osteoarthritis in a Thai population

Honsawek¹,

Deepaisarnsakul²,

Tanavalee³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Osteoarthritis is a chronic progressive degenerative joint disease characterized by age-related regressive change in articular cartilage. A single nucleotide polymorphism has been described at position -174 of the interleukin-6 (IL-6) promoter region, leading to three possible genotypes, GG, GC, and CC. We investigated a possible association of the IL-6 -174G/C gene polymorphism with knee osteoarthritis in a Thai population. Genotype distributions and allelic frequencies of the IL-6 -174G/C polymorphism were investigated in 115 knee osteoarthritis patients and 100 healthy controls. Genotyping was performed using PCR-RFLP. The genotype distribution of IL-6 was 79 GG, 36 GC, 0 CC in knee osteoarthritis patients and 88 GG, 12 GC, 0 CC in controls. The frequency of the GC genotype in subjects with knee osteoarthritis was higher than in controls (P < 0.001). Logistic regression analysis showed Association of IL-6 -174G/C polymorphism with knee OA that the GC genotype was independently associated with increased risk of knee osteoarthritis (odds ratio = 3.3, 95% confidence interval = 1.6-6.9, P = 0.001). These findings suggest that the -174G/C polymorphism of the IL-6 gene promoter plays a role in the pathogenesis of knee osteoarthritis.

show abstract

Effects of Interleukin‐6 on Proliferation and Proteoglycan Metabolism in Articular Chondrocyte Cultures

Cited by 54 publications

References 0 publications

ADAM‐12 (meltrin α) is involved in chondrocyte proliferation via cleavage of insulin‐like growth factor binding protein 5 in osteoarthritic cartilage

ADAM‐12 (meltrin α) is involved in chondrocyte proliferation via cleavage of insulin‐like growth factor binding protein 5 in osteoarthritic cartilage

Anti-Inflammation Effects of Low Intensity Laser Therapy on Monosodium Iodoacetate-induced Osteoarthritis in Rats

Association of the IL-6 -174G/C gene polymorphism with knee osteoarthritis in a Thai population

Contact Info

Product

Resources

About