Effects of interferon tau on endometrial epithelial cells in caprine in vitro

Zhang, Yue; Zhang, Lei; Yu, Chaofeng; Du, Xiaoyan; Liu, Xiaorui; Liu, Junze; An, Xiaopeng; Wang, Jiangang; Song, Yuxuan; Li, Guang; Cao, Binyun

doi:10.1016/j.gep.2017.06.010

Cited by 9 publications

(6 citation statements)

References 24 publications

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“…The expression of the NTRK2 gene, which encodes the receptor of brain derived neurotrophic factor, is conserved in mammalian uterus but its signaling function is not yet understood in the female reproductive system [ 71 ]. Genes known to be key regulators of uterine receptivity in different species such as, HOXA10 and HOXA11 belong also to the 50 genes top list characterizing ST as earlier shown in human [ 72 ], mice [ 73 ] and goat [ 74 ]. This list includes CALPAIN7 [ 75 ] and SNAI2 [ 76 ], involved in embryo attachment and implantation, and the disintegrins and metalloproteases ADAMTS1 and ADAM23 which encode key molecules for bovine endometrial remodelling [ 77 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells

et al. 2021

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Background A number of studies have examined mRNA expression profiles of bovine endometrium at estrus and around the peri-implantation period of pregnancy. However, to date, these studies have been performed on the whole endometrium which is a complex tissue. Consequently, the knowledge of cell-specific gene expression, when analysis performed with whole endometrium, is still weak and obviously limits the relevance of the results of gene expression studies. Thus, the aim of this study was to characterize specific transcriptome of the three main cell-types of the bovine endometrium at day-15 of the estrus cycle. Results In the RNA-Seq analysis, the number of expressed genes detected over 10 transcripts per million was 6622, 7814 and 8242 for LE, GE and ST respectively. ST expressed exclusively 1236 genes while only 551 transcripts were specific to the GE and 330 specific to LE. For ST, over-represented biological processes included many regulation processes and response to stimulus, cell communication and cell adhesion, extracellular matrix organization as well as developmental process. For GE, cilium organization, cilium movement, protein localization to cilium and microtubule-based process were the only four main biological processes enriched. For LE, over-represented biological processes were enzyme linked receptor protein signaling pathway, cell-substrate adhesion and circulatory system process. Conclusion The data show that each endometrial cell-type has a distinct molecular signature and provide a significantly improved overview on the biological process supported by specific cell-types. The most interesting result is that stromal cells express more genes than the two epithelial types and are associated with a greater number of pathways and ontology terms.

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Section: Discussionmentioning

confidence: 99%

Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells

et al. 2021

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“…The innate immune response mediated by interferon is a robust first line of defense against pathogens ( Schneider et al, 2014 ). Interferon signaling is important in immune response and inflammation ( Chen et al, 2017 ; Zhang et al, 2017 ). The abundance of interferon-stimulated genes in the downregulated DEG list, such as interferon-stimulated gene product 15 ( ISG15 ), IFI6 , MX1 , MX2 , DDX58 , IFIT3 , RSAD2 , IFI44L , IFIT2 , and IFI44 , indicates that miR-223 was likely to participate in immunity and inflammation.…”

Section: Discussionmentioning

confidence: 99%

EGF-Induced miR-223 Modulates Goat Mammary Epithelial Cell Apoptosis and Inflammation via ISG15

Zhang

Niu

et al. 2021

Front. Cell Dev. Biol.

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The health of mammary gland is essential for lactation. Epidermal growth factor (EGF) is reported to play an important role in lactation initiation and miR-223 is a conserved microRNA in anti-inflammation. In this study, EGF was found to induce a higher expression of miR-223 in goat mammary epithelial cell (gMEC). The downstream genes of miR-223 were screened by RNA sequencing, including Interferon-stimulated gene product 15 (ISG15), a pivotal immune responder, which was detected to be downregulated by EGF and miR-223. Due to the correlation between inflammation and apoptosis, the gMEC apoptosis modulated by EGF, miR-223, and ISG15 was investigated, and the protein expressions of Bcl-2/Bax, Caspase 3 and p53 were examined to evaluate the apoptosis of gMEC. The protein expressions of p-STAT3/STAT3, PR, FOXC1, and HOXA10, which had been shown to be related to inflammation, were detected to assess the inflammation of gMEC. This study provided a regulation axis, EGF/miR-223/ISG15, and illustrated its regulation to gMEC apoptosis and inflammation.

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“…The expression of the NTRK2 gene, which encodes the receptor of brain derived neurotrophic factor, is conserved in mammalian uterus but its signalling function is not yet understood in the female reproductive system [58]. Genes known to be key regulators of uterine receptivity in different species such as, HOXA10 and HOXA11 belong also to the top list of 50 genes which characterize ST (human [59], mice [60] and goat [61]). This list includes CALPAIN7 [62] and SNAI2 [63] which are involved in embryo attachment and implantation and the disintegrins and metalloproteases ADAMTS1 and ADAM23 which are genes encoding key molecules for bovine endometrial remodelling [64].…”

Section: Transcriptome Of the Three Endometrial Cell Typesmentioning

confidence: 99%

Impact of negative energy balance on transcriptomic profiles of three endometrial cell types isolated by laser capture microdissection in postpartum dairy cows

Chankeaw¹,

Lignier²,

Richard³

et al. 2020

Preprint

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Background: In postpartum dairy cows, the energy needs to satisfy high milk production induces a more or less pronounced Negative Energy Balance (NEB) status. NEB associated with fat mobilization impairs reproductive function. This study investigated the specific impact of NEB on gene expression in the three main types of endometrial cells at time planned for insemination and implantation. Endometrial cell types (stromal, glandular and luminal epithelial cells) were isolated by laser micro-dissection allowing the study of constitutive gene expression and their specific response to NEB. Methods: Nine Swedish Red cows receiving a control diet or a mild restricted diet to induce differences of energy balance were categorized into mild (MNEB, n = 5) and severe negative energy balance (SNEB, n = 4). The three endometrial cell types: luminal (LE), glandular (GE) epithelium and stroma (ST) were collected by laser microdissection from endometrial biopsies performed at 80 days postpartum. Results: Transcriptome profiles obtained by RNA sequencing revealed differences in constitutive gene expression between the three cells types and also differences in specific responses related to the severity of NEB. Number of differentially expressed genes between SNEB and MNEB cows was higher in ST than in LE and GE, respectively. SNEB was associated with differential expression of genes related to metabolic processes and embryo-maternal interactions in ST. Under-expression of genes related to cell structure was found in GE whereas genes related to pro-inflammatory pathways were over-expressed. Genes associated to adaptive immunity were under-expressed in LE. Conclusion: The three different main cells types of the endometrium, have very different patterns of gene expression. The severity of NEB after calving is associated with changes in gene expression at time of breeding. Specific alterations in GEs are associated with activation of pro-inflammatory mechanisms. Concomitantly, changes in the expression of genes related to cell to cell interactions and maternal recognition of pregnancy takes place in ST. The combination of these effects possibly altering the uterine environment and embryo maternal interactions may negatively influence the establishment of pregnancy.

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Effects of interferon tau on endometrial epithelial cells in caprine in vitro

Cited by 9 publications

References 24 publications

Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells

Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells

EGF-Induced miR-223 Modulates Goat Mammary Epithelial Cell Apoptosis and Inflammation via ISG15

Impact of negative energy balance on transcriptomic profiles of three endometrial cell types isolated by laser capture microdissection in postpartum dairy cows

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