Effects of interactions among Metarhizium anisopliae, Bacillus thuringiensis and chlorantraniliprole on the mortality and pupation of six geographically distinct Helicoverpa armigera field populations

Wakil, Waqas; Ghazanfar, Muhammad Usman; Riasat, Tahira; Qayyum, Mirza Abdul; Ahmed, Sohail; Yasin, Muhammad

doi:10.1007/s12600-012-0282-9

Cited by 25 publications

(28 citation statements)

References 41 publications

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“…Concentration of spore-crystal mixture was estimated by the optical density at 600 nm (Hernández et al, 2005) in 1:100 dilutions. Toxin obtained was then stored in the refrigerator until used (Wakil et al, 2013).…”

Section: Bacillus Thuringiensis Toxinmentioning

confidence: 99%

Bacillus thuringiensis and Nuclear Polyhedrosis Virus for the Enhanced Bio-control of Helicoverpa armigera

Qayyum¹,

Wakil²,

Arif³

et al. 2015

IJAB

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A number of entomopathogenic microbial agents infect a wide array of insects and cause epizootics from time to time. Bacillus thuringiensis (Bt) and Nuclearpolyhedrosis virus (NPV) are two important potential pathogens of Helicoverpa armigera. Investigations were carried out on integration of B. thuringiensis and Nuclearpolyhedrosis virus to determine its impact on survival of second and fourth instar larvae under laboratory conditions. Both Bt and NPV were applied using diet incorporation method. Larvae continued to feed for 48 h when a batch of fifteen insects was exposed to diet mixed with any of the treatment. Mortality was recorded every 48 h until larvae died or pupated. The susceptibility of H. armigera larvae decreased with older stage as greater mortality was recorded in second instar larvae (95.45%) in comparison to fourth instar larvae (82.48%) in combined application of Bt and NPV. Application rate of Bt was the key for type of interaction as only one synergistic interaction (CTF≥20) was recorded when lower dose of Bt was combined with NPV, rest of the combinations were additive and independent (CTF≤20). Reduction in the pupation, adult emergence and egg eclosion was found directly related to pathogenicity of the applied agents. This study would be helpful to extend the integrated use of pathogens under field condition to many important insect pests.

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Section: Bacillus Thuringiensis Toxinmentioning

confidence: 99%

Bacillus thuringiensis and Nuclear Polyhedrosis Virus for the Enhanced Bio-control of Helicoverpa armigera

Qayyum¹,

Wakil²,

Arif³

et al. 2015

IJAB

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“…was provided by BioSciences Corporation, Libertyville, IL, USA. The B. thuringiensis toxin previously extracted (Wakil et al, 2013) was used in the bioassays @ 0.5 µg g-1 against E. vittella.…”

Section: B Thuringiensis Toxinmentioning

confidence: 99%

“…B. thuringiensis toxin enters the insect through ingestion and binds to glycoprotein receptors of the targeted insect midgut epithelium, where they disrupt the cytoplasmic membrane, leading to the cell lysis (Hilder and Boulter, 1999). In number of studies, B. thuringiensis intoxication enhanced the efficacy of B. bassiana especially in case of Lepidopterous insect pests when commercial formulation of B. thuringiensis was combined with B. bassiana (Gao et al, 2012;Wakil et al, 2013). The increasing interest in combining sub-lethal concentrations of B. thuringiensi with B. bassaina has stemmed from their successful control against different insect pests (Ma et al, 2008;Furlong and Groden, 2003).…”

Section: Introductionmentioning

confidence: 99%

Control of Earias vittella (Lepidoptera: Noctuidae) by Beauveria bassiana along with Bacillus thuringiensis

Ali¹,

Wakil²,

Zia³

et al. 2015

IJAB

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A laboratory strain of Beauveria bassiana sensu lato (Balsamo) Vuillemin (Hypocreales: Clavicipitaceae) and commercial formulation of Bacillus thuringiensis (Berliner) were assessed against three field populations of Earias vittella F. ) were applied alone and in combination against 2 nd and 4 th larval instars. The mortality was observed until pupation. The bioassays were carried out at 25°C and 75% RH The highest larval mortality was observed in the population from Faisalabad with lowest pupation rate, adult emergence and egg eclosion treated with combined concentrations of B. bassiana and B. thuringiensis. The lowest mortality was observed in population from Pakpattan among all the tested populations. Overall results demonstrated that all the treatments gave significant control of E. vittella and both microbial agents may become the integral part of the successful IPM program of E. vittella in okra crop.

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“…Entomopathogenic fungi can control pests, and Beauveria spp., Metarhizium spp., and Nomuraea spp. (Hypocreales: Clavicipitaceae) are considered worldwide for control of H. armigera (Nguyen et al 2007;Hatting 2012;Wakil et al 2013). The integrated management of H. armigera should include identification of natural enemies in the field.…”

mentioning

confidence: 99%

“…Nomuraea rileyi is known to infect and cause mortality in insects (Hatting 2012; Wakil et al 2013), with several N. rileyi-lepidopteran associations reported from the South American continent. These include Anticarsia gemmatalis Hübner, Trichoplusia ni Hübner, and Alabama argillacea Hübner (Lepidoptera: Noctuidae) (Corrêa & Smith 1975;Alves et al 1978;Villani et al 1984;Sujii et al 2002).…”

mentioning

confidence: 99%

Comparison of Three Entomopathogenic Fungi Against the Bollworm,Helicoverpa armigera(Hübner) (Lepidoptera: Noctuidae), Employing Topicalvs per osInoculation Techniques

Hatting

2012

African Entomology

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Effects of interactions among Metarhizium anisopliae, Bacillus thuringiensis and chlorantraniliprole on the mortality and pupation of six geographically distinct Helicoverpa armigera field populations

Cited by 25 publications

References 41 publications

Bacillus thuringiensis and Nuclear Polyhedrosis Virus for the Enhanced Bio-control of Helicoverpa armigera

Bacillus thuringiensis and Nuclear Polyhedrosis Virus for the Enhanced Bio-control of Helicoverpa armigera

Control of Earias vittella (Lepidoptera: Noctuidae) by Beauveria bassiana along with Bacillus thuringiensis

Comparison of Three Entomopathogenic Fungi Against the Bollworm,Helicoverpa armigera(Hübner) (Lepidoptera: Noctuidae), Employing Topicalvs per osInoculation Techniques

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