Effects of insulin treatment on hepatic CYP1A1 and CYP2E1 activities and lipid peroxidation levels in streptozotocin-induced diabetic rats

Kuzgun, Gökçe; Başaran, Rahman; İnan, Ebru Arıoğlu; Can‐Eke, Benay

doi:10.1007/s40200-020-00616-y

Cited by 15 publications

(17 citation statements)

References 39 publications

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“…The novelty of the present study is that cytochrome p450 was detected in blood with this spectroscopy, which allows the deconvolution of the blood optical absorption spectra, due to the simultaneously detection of PA amplitude and phase, also without any preparation of the sample. Moreover, Table I shows that there was a significant correlation between the area under the curve of cytochrome p450 and hyperglycemia, according to the findings reported by Zong et al, 49 Raza et al, 50 and Kuzgun et al 51 Cytochrome p450 is an important component of enzymes that are membrane-bound hemoproteins that play an important role in detoxification (endogenous and exogenous compounds), metabolism, and homeostasis. 52 Cytochrome p450 seems to have an important role in diabetes.…”

Section: Glucose and Weightsupporting

confidence: 59%

Photoacoustic Spectroscopy and Hyperglycemia in Experimental Type 1 Diabetes

et al. 2021

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According to the World Health Organization, diabetes was the seventh leading cause of death in 2016. Long-term diabetes complications are associated with hyperglycemia. It is difficult to predict the beginning and evolution of those complications. The goal of the study was to evaluate the relationship between glycemia and blood spectroscopic variables in an experimental model of type 1 diabetes (streptozotocin model). Blood samples were taken weekly (10 weeks) from the tail of male Wistar rats with or without diabetes. Blood optical absorption spectra were obtained by means of photoacoustic spectroscopy. It was possible to estimate the time-course of blood characteristic peak ratios. The area under the curve of those peaks correlated with hyperglycemia. The evolution of the optical absorption at 450 nm, related to cytochrome p450, was obtained by using the phase-resolved method. The area under the curve of p450 correlated also with hyperglycemia. It is concluded that photoacoustic spectroscopy is a reliable technology to detect the effects of hyperglycemia on blood with possible applications in the study of long-term diabetes complications.

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Section: Glucose and Weightsupporting

confidence: 59%

Photoacoustic Spectroscopy and Hyperglycemia in Experimental Type 1 Diabetes

et al. 2021

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“…CYP2E1 is an inducible gene that is upregulated under multiple conditions, such as fasting and nutrition intake, as well as in a wide variety of pathophysiological states (2,(12)(13)(14)(15)(16)(17). In addition to metabolizing endogenous substrates and xenobiotics, CYP2E1 is the main source of cellular ROS, and its NADPH oxidase activity is higher than that of other CYP family members (9)(10)(11).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, it can catalyze the reaction of NADPH and O 2 to generate reactive oxygen species (ROS) (7,8). CYP2E1 is the major enzyme for the production of cellular and mitochondrial ROS and reactive nitrogen species (RNS), which induce tissue damage mainly by damaging cellular and mitochondrial macromolecules, including mitochondrial DNA (9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Diallyl sulfide protects against dilated cardiomyopathy via inhibition of oxidative stress and apoptosis in mice

et al. 2021

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Cytochrome P450 family 2 subfamily E member 1 (CYP2E1) is a member of the cytochrome P450 enzyme family and catalyzes the metabolism of various substrates. CYP2E1 is upregulated in multiple heart diseases and causes damage mainly via the production of reactive oxygen species (ROS). In mice, increased CYP2E1 expression induces cardiac myocyte apoptosis, and knockdown of endogenous CYP2E1 can attenuate the pathological development of dilated cardiomyopathy (DCM). Nevertheless, targeted inhibition of CYP2E1 via the administration of drugs for the treatment of DCM remains elusive. Therefore, the present study aimed to investigate whether diallyl sulfide (DAS), a competitive inhibitor of CYP2E1, can be used to inhibit the development of the pathological process of DCM and identify its possible mechanism. Here, cTnT R141W transgenic mice, which developed typical DCM phenotypes, were used. Following treatment with DAS for 6 weeks, echocardiography, histological analysis and molecular marker detection were conducted to investigate the DAS-induced improvement on myocardial function and morphology. Biochemical analysis, western blotting and TUNEL assays were used to detected ROS production and myocyte apoptosis. It was found that DAS improved the typical DCM phenotypes, including chamber dilation, wall thinning, fibrosis, poor myofibril organization and decreased ventricular blood ejection, as determined using echocardiographic and histopathological analyses. Furthermore, the regulatory mechanisms, including inhibition both of the oxidative stress levels and the mitochondria-dependent apoptosis pathways, were involved in the effects of DAS. In particular, DAS showed advantages in terms of improved chamber dilation and dysfunction in model mice, and the improvement occurred in the early stage of the treatment compared with enalaprilat, an angiotensin-converting enzyme inhibitor that has been widely used in the clinical treatment of DCM and HF. The current results demonstrated that DAS could protect against DCM via inhibition of oxidative stress and apoptosis. These findings also suggest that inhibition of CYP2E1 may be a valuable therapeutic strategy to control the development of heart diseases, especially those associated with CYP2E1 upregulation. Moreover, the development of DAS analogues with lower cytotoxicity and metabolic rate for CYP2E1 may be beneficial.

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“…Excessive oxidation products are harmful to proteins, lipids, and nucleic acids, leading to the damage to cellular structure and function (Cháfer-Pericás, 2021). Thereby, reactive oxygen species-induced lipid peroxidation could contribute to chronic diseases, such as cardiovascular disease, diabetes, cancer, and senescence (Clemente et al, 2020;Gianazza et al, 2020;Kuzgun et al, 2020). Due to the side effects of drug treatment on dyslipidemia and lipid peroxidation, finding bioactive compounds from natural products has attracted more and more attention.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Ellagic Acid on Hepatic Lipid Metabolism and Antioxidant Activity in Mice

Tang

et al. 2021

Front. Physiol.

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Accumulating evidence has demonstrated that the imbalance of lipid metabolism and antioxidant capacity leads to damage to liver. The present study aimed to investigate the effects of ellagic acid (EA), a phenolic compound, on hepatic lipid metabolism and antioxidant activity in mice. In our study, 24 C57BL/6J mice were divided into three groups: (1) control (CON); (2) basal diet+0.1% EA (EA1); and (3) basal diet+0.3% EA (EA2). After the 14-day experiment, the liver was sampled for analysis. The results showed that 0.3% EA administration increased the liver weight. Total cholesterol and low-density lipoprotein cholesterol activities decreased and high-density lipoprotein cholesterol activity increased by EA supplementation. Meanwhile, dietary supplementation with EA dose-dependently decreased the acetyl-CoA carboxylase protein abundance and increased the phospho-hormone-sensitive lipase, carnitine palmitoyltransferase 1B, and peroxisome proliferator-activated receptor alpha protein abundances. Moreover, EA supplementation reduced the malonaldehyde concentration and increased the superoxide dismutase and catalase concentrations. The protein abundances of phospho-nuclear factor-E2-related factor 2, heme oxygenase-1, and NAD(P)H: quinone oxidoreductase 1 increased by EA supplementation in a dose-dependent manner. Taken together, EA supplementation promoted the lipid metabolism and antioxidant capacity to maintain the liver health in mice.

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Effects of insulin treatment on hepatic CYP1A1 and CYP2E1 activities and lipid peroxidation levels in streptozotocin-induced diabetic rats

Cited by 15 publications

References 39 publications

Photoacoustic Spectroscopy and Hyperglycemia in Experimental Type 1 Diabetes

Photoacoustic Spectroscopy and Hyperglycemia in Experimental Type 1 Diabetes

Diallyl sulfide protects against dilated cardiomyopathy via inhibition of oxidative stress and apoptosis in mice

The Effect of Ellagic Acid on Hepatic Lipid Metabolism and Antioxidant Activity in Mice

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