Effects of Insulin-Like Growth Factor-1 and Donor Age on Transplantation of Porcine Neonatal Pancreatic Cell Clusters

Juang, Jyuhn‐Huarng; Kuo, Chun‐Yan; Yao, Nan-Kuang

doi:10.1016/j.transproceed.2009.03.057

Cited by 4 publications

(10 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 ); better understanding of cellular and molecular changes of NPCCs post-Tx would potentially be critical to define important intrinsic and external players to facilitate Tx outcome (e.g. to shorten time window of glycemic normalization post Tx) 14 , 26 . Furthermore, the underlying mechanisms of immune rejections upon NPCC xenotransplantation could also possibly be addressed if impacts of adjacent microenvironment of NPCCs-bearing grafts are elucidated 27 .…”

Section: Resultsmentioning

confidence: 99%

“…Based on our present data, short-term NPCC culture as well as long-term NPCC-bearing grafts could be ideal platforms to more convincingly test insulinotropic agents for porcine/human pancreatic precursor-like cells from postnatal pancreas. Previous studies, however, still failed to enhance mature β-cell characteristics in NPCC culture supplemented with GLP-1 15 or IGF-1 alone 13 compared with control group in vitro ; or to improve Tx outcome by shortening the latent period between Tx and reversal of hyperglycemia in vivo 13 , 14 . These results indicate a great demand to profile molecular changes of PDX1 + /SOX9 + and insulin + cells in NPCC-bearing grafts dynamically.…”

Section: Discussionmentioning

confidence: 99%

“…Series of studies have tested the role of potential islet differentiation triggers such as glucagon-like peptide 1 (GLP-1) 10 , cholecystokinin (CCK) 11 and insulin-like growth factor-1 (IGF-1) 12 in promoting maturation of fetal islet-bearing grafts either in vitro 13 , 14 or in vivo 11 , 15 aiming to shorten the latent period between Tx and reversal of hyperglycemia. The results were inconsistent and showed a minimal improvement of Tx outcomes in response to these maturation promoters, further arguing a requirement to clarify cellular identity of initial transplants as well as to define dynamic molecular basis regarding how fetal islets differentiate in culture and after Tx.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Porcine Neonatal Pancreatic Cell Clusters Maintain Their Multipotency in Culture and After Transplantation

Li¹,

Chen

Kao

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Ductal epithelium is primarily detected in porcine neonatal pancreatic cell clusters (NPCCs) bearing grafts, suggesting that transplants might exhibit progenitor-like phenotypes. Here we found that soon after NPCC isolation, PDX1+/insulin− and SOX9+ pancreatic progenitor-like cells dramatically increased while dual-hormonal progenitor-like cells were routinely observed in NPCC culture. After transplantation (Tx), insulin+ cells increased and PDX1+ and SOX9+ cells gradually decreased in both non-diabetic (NDM) and streptozotocin-induced diabetic (DM) grafts over 2 months. Strikingly, a significantly higher percentage of insulin+ cells were detected in 9-day and 16-day, but not in 23-day, 30-day and 60-day grafts implying that hyperglycemia could only facilitate NPCC-derived β cells early post-Tx. A higher percentage of NPCC-derived β cells in early DM grafts was determined via an enhanced neogenic differentiation based on the detection of insulin+ cells budding out from PDX1+/SOX9+ epithelium. Interestingly, a drop in SOX9+ progenitor-like cells was detected 16 days post-Tx in DM grafts whilst PDX1+ cells do not show a significant difference until 60 days post-Tx between DM and NDM grafts, demonstrating that distinct progenitor-like populations fuel new β cells post-Tx. In conclusion, PDX1+/SOX9+ cells could be quickly activated after NPCC isolation, maintain their multipotency in culture and differentiate into new β cell post-Tx.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Porcine Neonatal Pancreatic Cell Clusters Maintain Their Multipotency in Culture and After Transplantation

Li¹,

Chen

Kao

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Each neonatal pig pancreas was cut into fragments from~1 to 2 mm 3 , then digested by collagenase type V in a water bath at 37 • C. The digest was filtered, washed, then placed in RPMI-1640 medium and maintained at 37 • C (5% CO 2 , 95% air) in humidified air [12]. After 3 days of culture, NPCCs were incubated overnight with CSPIO nanoparticles (concentration of 10.08 µg/mL) before in vitro studies and transplantation.…”

Section: Preparation and Culture Of Npccsmentioning

confidence: 99%

“…However, adult porcine islets are fragile during isolation [6], and fetal islets have a poor insulin response to glucose [6,7]. In contrast, porcine neonatal pancreatic cells (NPCCs) are easily isolated, and capable of secreting insulin in response to glucose and restoring normoglycemia after transplantation Polymers 2021, 13, 1238 2 of 10 in diabetic mice [8][9][10][11][12], pigs [13], and nonhuman primates [14]. However, they are rather immature and continue differentiation in vitro [8,9,15] and in vivo [9,10,15].…”

Section: Introductionmentioning

confidence: 99%

Magnetic Resonance Imaging of Transplanted Porcine Neonatal Pancreatic Cell Clusters Labeled with Chitosan-Coated Superparamagnetic Iron Oxide Nanoparticles in Mice

et al. 2021

View full text Add to dashboard Cite

Neonatal pancreatic cell clusters (NPCCs) are potential tissues for the treatment of diabetes. Different from adult cells, they continuously proliferate and differentiate after transplantation. In this study, we utilized magnetic resonance imaging (MRI) to detect and monitor implanted NPCCs. NPCCs were isolated from one-day-old neonatal pigs, cultured for three days, and then incubated overnight with the contrast agent chitosan-coated superparamagnetic iron oxide (CSPIO) nanoparticles. In vitro, Prussian blue staining and MR scans of CSPIO-labeled NPCCs were performed. In vivo, we transplanted 2000 CSPIO-labeled NPCCs under the kidney capsule of nondiabetic nude mice. Recipients were scanned with 7.0T MRI. Grafts were removed for histology with insulin and Prussian blue staining. After being incubated overnight with CSPIO, NPCCs showed positive iron staining and appeared as dark spots on MR scans. After transplantation of CSPIO-labeled NPCCs, persistent hypointense areas were observed at recipients’ implant sites for up to 54 days. Moreover, histology showed colocalization of the insulin and iron staining in 15-, 51- and 55-day NPCC grafts. Our results indicate that transplanted NPCCs survived and differentiated to β cells after transplantation, and that MRI is a useful tool for the detection and monitoring of CSPIO-labeled NPCC grafts.

show abstract

Modest Effects of Fas-Ligand and Heme Oxygenase-1 Double Transgenic Mouse Islets on Transplantation Outcomes

Juang¹,

Tu²,

Kuo³

2011

Transplantation Proceedings

View full text Add to dashboard Cite

Effects of Insulin-Like Growth Factor-1 and Donor Age on Transplantation of Porcine Neonatal Pancreatic Cell Clusters

Cited by 4 publications

References 13 publications

Porcine Neonatal Pancreatic Cell Clusters Maintain Their Multipotency in Culture and After Transplantation

Porcine Neonatal Pancreatic Cell Clusters Maintain Their Multipotency in Culture and After Transplantation

Magnetic Resonance Imaging of Transplanted Porcine Neonatal Pancreatic Cell Clusters Labeled with Chitosan-Coated Superparamagnetic Iron Oxide Nanoparticles in Mice

Modest Effects of Fas-Ligand and Heme Oxygenase-1 Double Transgenic Mouse Islets on Transplantation Outcomes

Contact Info

Product

Resources

About