Effects of Insulin and Muscular Exercise upon the Uptake of Hexoses by Muscle Cells

Huycke, E. J.; Kruhøffer, P.

doi:10.1111/j.1748-1716.1955.tb01243.x

Cited by 22 publications

(7 citation statements)

References 8 publications

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“…In fed and starved rats, exercise caused an 8-12-fold increase in glucose uptake when insulin was not added to the perfusate (Table 3). This is in accordance with earlier work carried out with exercising hindlimb and hindquarter preparations (McGuigan, 1908;Huycke & Kruhoffer, 1955;Szabo et al, 1969;Ruderman et al, 1971). In the presence of added insulin, exercise caused a further increase in the uptake of glucose from 5.2±0.4 to 8.4±0.6,umol/min per 30g of muscle in fed rats and from 5.4±0.7 to 8.8±0.6,umol/min per 30g of muscle in starved rats.…”

Section: Glucose Uptake By Exercising Musclesupporting

confidence: 93%

Glucose metabolism in perfused skeletal muscle. Interaction of insulin and exercise on glucose uptake

Berger

Hagg

Ruderman

1975

Biochemical Journal

206

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1. The interaction of insulin and isometric exercise on glucose uptake by skeletal muscle was studied in the isolated perfused rat hindquarter. 2. Insulin, 10 m-i.u./ml, added to the perfusate, increased glucose uptake more than 10-fold, from 0.3-0.5 to 5.2-5.4 mumol/min per 30g of muscle in hindquarters of fed and 48h-starved rats respectively. In contrast, it did not stimulate glucose uptake in hindquarters from rats in diabetic ketoacidosis. 3. In the absence of added insulin, isometric exercise, induced by sciatic-nerve stimulation, increased glucose uptake to 4 and 3.4 mumol/min per 30g of muscle in fed and starved rats respectively. It had a similar effect in rats with moderately severe diabetes, but it did not increase glucose uptake in rats with diabetic ketoacidosis or in hindquarters of fed rats that had been "washed out" with an insulin-free perfusate. Insulin, at concentrations which did not stimulate glucose uptake in resting muscle, restored the stimulatory effect of exercise in these situations. 4. The stimulation of glucose uptake by exercise was independent of blood flow and the degree of tissue hypoxia; also it could not be reproduced by perfusing resting muscle with a medium previously used in an exercise experiment. 5. At rest glucose was not detectable in muscle cell water of fed and starved rats even when perfused with insulin. In the presence of insulin, a small accumulation of glucose, 0.25 mM, was noted in the muscle of ketoacidotic diabetic rats, suggesting inhibition of glucose phosphorylation, as well as of transport. 6. During exercise, the calculated intracellular concentration of glucose in the contracting muscle increased to 1.1-1.6mM in the fed, starved and moderately diabetic groups. Insulin significantly increased the already high rates of glucose uptake by the hindquarters of these animals but it did not alter the elevated intracellular concentration of glucose. 7. In severely diabetic rats, exercise did not cause glucose to accumulate in the cell in the absence of insulin. In the presence of insulin, it increased glucose uptake to 6.1 mumol/min per 30g of muscle and intracellular glucose to 0.72 mM. 8. The data indicate that the stimulatory effect of exercise on glucose uptake requires the presence of insulin. They suggest that in the absence of insulin, glucose uptake is not enhanced by exercise owing to inhibition of glucose transport into the cell.

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Section: Glucose Uptake By Exercising Musclesupporting

confidence: 93%

Glucose metabolism in perfused skeletal muscle. Interaction of insulin and exercise on glucose uptake

Berger

Hagg

Ruderman

1975

Biochemical Journal

206

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“…Increased glucose disappearance during exercise was demonstrated to be due to enhanced glucose transport, increased glucose utilization, and expansion of glucose space, both in vivo (Goldstein, Mullick, Huddlestun and Levine, 1953;Sanders, Levinson, Abelmann and Freinkel, 1964;Dulin and C7ark, 1961 ;Helmreich and Cori, 1957) and in vitro (Huycke and Kruhoffer, 1955;Holloszy and Narahara, 1967). As skeletal muscle is quantitatively the most voluminous tissue of the mammalian organism (Andres, Cader and Zierler, 1956) even a small change in the rate of glucose utilization by this tissue may be a satisfactOlY explanation for the lowering of blood sugar levels during exercise.…”

Section: Introductionmentioning

confidence: 99%

Glucose Uptake by the Isolated Perfused Rat Hind Limb during Rest and Exercise

Aj¹,

Rj²,

Szabó³

1969

Horm Metab Res

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Perfusion of the isolated, rat hind limb was performed during conditions of rest and exercise. The rate of blood tlow increased progressively from onset of perfusion in both the control and stimulated preparations with no significant difference between the two groups, whereas glucose disappearance from the perfusate was greatly accelerated during exercise. Tbe exercised limb extracted more glucose from the blood than the control preparation, in spite of a lower glucose concentration and a diminished volume of perfusate passing through the musc1e during the same period of time. Glucose uptake remained elevated during the entire period of electrical stimulation of the motor nerves, even though the amplitude of musc1e movements gradually dirninished with time.

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“…In early studies (Huycke & Kruhoffer, 1955;Holloszy & Narahara, 1965;Ivy & Holloszy, 1981), curiosity about the benefi t of exercise on diabetes stirred interest in examining that relationship using animal models. Employing rats, dogs, and frogs as subjects, studies in the 1950s to 1980s examined the effects of exercise on glucose homeostasis (i.e., blood sugar balance).…”

Section: Scientifi C Insightsmentioning

confidence: 98%