Effects of insulin and metformin on fetal kidney development of streptozotocin-induced gestational diabetic albino rats

Kassab, Ban M.; Raouf, Hoda H. Hussein Abd El; Mahmoud, Omayma M.; Abdel-Alrahman, Gamal

doi:10.5115/acb.2019.52.2.161

Cited by 11 publications

(5 citation statements)

References 49 publications

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“…Furthermore, the diabetic control group showed increased tubular injury score due to vacuolations, desquamation, and dilatation of both PCTs and DCTs. SO, the renal parameters showed moderate improvement in the aniseed treated group, mild in the insulin-treated diabetic group, and marked amelioration in the anise plus insulin group and this is in agreement with [39] who found the combination of insulin and metformin produces the best way in glucose control and diminished the renal damage by STZ induced gestational diabetes.…”

Section: Discussionsupporting

confidence: 90%

The protective effect of anise, insulin, and their combination in the nephropathy of diabetic rat model

Ragab

El-Habiby

El-Mehi

et al. 2020

Egyptian Journal of Histology

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Introduction: Among the diabetic complications, the diabetic nephropathy (DN) is the commonest reason for end-stage kidney failure all over the world, and a major indication for dialysis and transplantation. Despite the number of studies on humans and rodents of experimental DN, successful treatment isn't accessible however So, the explore for drugs to prevent the progression of DN has a high priority in biomedical research Aim: To examine the effect of anise on a diabetic nephropathy rat model by streptozotocin and compare its effect with insulin and with their combination. Materials and Methods: Fifty-six adult male albino rats were divided into six main groups. Group I (control), Group II: received aniseed at a dose of 500 mg/kg, Group III (diabetic): received a single IP of 50 mg/kg STZ, Group IV: diabetic rats received aniseed for eight weeks (as group II), Group V: received SC of 2U/Kg once daily of an insulin mixture for eight weeks and Group VI: received aniseed and insulin as the previous doses for eight weeks. At the end of the study, blood samples were collected and the kidney specimens were processed biochemical, histological (light and transmission electron microscopic) and immunohistochemical studies. Morphometric and statistical studies were also performed. Results: Diabetic rats showed disturbed renal architecture involving the corpuscles and tubules, a significant increase in collagen fibers, glomerulosclerosis index, and desmin, VEGF, keratin 18immunoreaction. Administration of aniseed showed impressive improvement of histological structural changes induced by diabetes, insulin showed a mild improvement and use of these agents in combination appeared to have potential advantages especially in reducing of oxidative stress in improving DN Conclusions: A combination of aniseed and insulin have a relevant therapeutic role in the kidney changes induced by diabetes So, they could potentially provide a possible solution for renal changes related to diabetes

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Section: Discussionsupporting

confidence: 90%

The protective effect of anise, insulin, and their combination in the nephropathy of diabetic rat model

Ragab

El-Habiby

El-Mehi

et al. 2020

Egyptian Journal of Histology

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“…S4 F), which was inconsistent with other studies. 42 , 43 After 2-month metformin gavage, the morphology of the MG was relatively well-maintained ( Figs. 6 E, 6 F), and the acini of rats applied Met for 2 months (the Met4M group) displayed normal structure as the normal control (NT4M) group ( Fig.…”

Section: Resultsmentioning

confidence: 98%

Hyperglycemia Induces Meibomian Gland Dysfunction

Guo

Zhang

Zhao

et al. 2022

Invest. Ophthalmol. Vis. Sci.

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Purpose Patients diagnosed with diabetes are inclined to have abnormalities on stability of tear film and disorder of meibomian gland (MG). This study aims to explore the pathological change of MG induced by diabetes in a rat model. Methods Sprague-Dawley (SD) rats were intraperitoneally injected with streptozotocin (STZ) to establish a diabetic animal model. Lipid accumulation in MG was detected by Oil Red O staining and LipidTox staining. Cell proliferation status was determined by Ki67 and P63 immunostaining, whereas cell apoptosis was confirmed by TUNEL assay. Gene expression of inflammatory cytokines and adhesion molecules IL-1α, IL-1β, ELAM1, ICAM1, and VCAM1 were detected by RT-PCR. Activation of ERK, NF-κB, and AMPK signaling pathways was determined by Western Blot analysis. Oxidative stress-related factors NOX4, 4HNE, Nrf2, HO-1, and SOD2 were detected by immunostaining or Western Blot analysis. Tom20 and Tim23 immunostaining and transmission electron microscopy were performed to evaluate the mitochondria functional and structure change. Results Four months after STZ injection, there was acini dropout in MG of diabetic rats. Evident infiltration of inflammatory cells, increased expression of inflammatory factors, and adhesion molecules, as well as activated ERK and NF-κB signaling pathways were identified. Oxidative stress of MG was evident in 4-month diabetic rats. Phospho-AMPK was downregulated in MG of 2-month diabetic rats and more prominent in 4-month rats. After metformin treatment, phospho-AMPK was upregulated and the morphology of MG was well maintained. Moreover, inflammation and oxidative stress of MG were alleviated after metformin intervention. Conclusions Long-term diabetes may lead to Meibomian gland dysfunction (MGD). AMPK may be a therapeutic target of MGD induced by diabetes.

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“… 12 , 41 , 42 , 43 , 44 GDM is traditionally defined as any grade of glucose intolerance during pregnancy. 45 , 46 …”

Section: Discussionmentioning

confidence: 99%

Are miR‐26a and miR‐26b microRNAs potent prognostic markers of gestational diabetes?

Ghaneialvar,

Mohseni,

Kenarkoohi

et al. 2024

Health Science Reports

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BackgroundGestational diabetes mellitus is a common public health problem, accompanied by complications for the mother and fetus. So, introducing new biomarkers to identify early diabetes is essential. As serum miRNAs are potentially appropriate markers, we investigated miR‐26a and miR‐26b expression levels in pregnant women with and without gestational diabetes.MethodDemographic and clinical characteristics of 40 gestational diabetic patients and 40 healthy controls were assessed. The expression level of miR‐26a and miR‐26b microRNAs was measured by real‐time PCR. Statistical analysis was done with GraphPad Prism software (version 8.4.3).ResultThe findings of this study showed that the expression level of miR‐26a and miR‐26b increased in women with gestational diabetes compared with healthy pregnant women, but the increase in expression was only significant for miR‐26a (p < 0.05).ConclusionAccording to the statistical and ROC curves, we suggest miR‐26a as a potential biomarker for the early diagnosis of gestational diabetes mellitus.

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Effects of insulin and metformin on fetal kidney development of streptozotocin-induced gestational diabetic albino rats

Cited by 11 publications

References 49 publications

The protective effect of anise, insulin, and their combination in the nephropathy of diabetic rat model

The protective effect of anise, insulin, and their combination in the nephropathy of diabetic rat model

Hyperglycemia Induces Meibomian Gland Dysfunction

Are miR‐26a and miR‐26b microRNAs potent prognostic markers of gestational diabetes?

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