Effects of Inositol Tri‐, Tetra‐, Penta‐, and Hexaphosphates on <i>In Vitro</i> Estimation of Iron Availability

Sandberg, Ann‐Sofie; Carlsson, Nils‐Gunnar; Svanberg, Ulf

doi:10.1111/j.1365-2621.1989.tb08591.x

Cited by 161 publications

(129 citation statements)

References 19 publications

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“…The amounts of zinc and calcium are given in Table 1. The amounts of inositol tri-to hexaphosphates (InsP 3 -InsP 6 ) in the barley products were determined by ion-pair C 18 reverse-phase HPLC using formic acid and methanol and tertrabutylammoniumhydroxide in the mobile phase according to Sandberg and Ahderinne (1986) and Sandberg et al (1989). The HPLC comprised of an HPLC pump (Waters Model 510, Waters Associates Inc., MA, USA), a C 18 Chromasil (5 U) column 2 mm i.d.…”

Section: Chemical Measurementsmentioning

confidence: 99%

Hydrothermal treatment and malting of barley improved zinc absorption but not calcium absorption in humans

et al. 2003

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Objective: To study whether hydrothermal treatment or malting of barley (cv. Blenheim) improves zinc and calcium absorption in humans. Design: Two groups of 10 and 12 healthy subjects, respectively, were in a period of 2 months in a fasting state, served two single meals each containing porridge or breakfast cereals prepared from processed or unprocessed (control) barley (60 g). The meals included 200 g of milk, extrinsically labelled with 65 Zn and 47 Ca. Whole-body retention of both minerals was measured. Setting: The study was carried out at the Department of Radiation Physics, Sahlgrenska University Hospital, Göteborg. Subjects: The subjects were recruited among students at the Göteborg University. None dropped out. Interventions: The activities of 65 Zn and 47 Ca were measured by whole-body counting four to five times over a 4-week period after each meal. Results: Zinc absorption from hydrothermally treated barley porridge, containing 28 mg P as inositol tri-to hexaphosphates (InsP 3 -InsP 6 ), was significantly higher (Po0.001) than from control porridge containing 111 mg P as InsP 3 -InsP 6 , 25.276.9 vs 11.072.5% (n ¼ 12). Calcium absorption did not differ (P40.05), 21.176.8 vs 19.574.7% (n ¼ 12). Zinc absorption from breakfast cereals of malted barley with phytase activity and containing 70 mg P as InsP 3 -InsP 6, was significantly higher (Po0.05) than from flakes of barley, containing 108 mg P as InsP 3 -InsP 6 and no phytase activity, 22.975.8 vs 14.874.6% (n ¼ 10). The calcium absorption was 21.376.5 vs 18.574.3% (n ¼ 10) and did not differ significantly (P40.05).

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Section: Chemical Measurementsmentioning

confidence: 99%

Hydrothermal treatment and malting of barley improved zinc absorption but not calcium absorption in humans

et al. 2003

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“…LEHRFELD [24] e SANDBERG et al [40] já haviam demonstrado o uso de par iônico na fase reversa para identificar e quantificar este composto e seus derivados desfosforilados, concluindo que suas separações e quantificações foram eficientes. MAYR [30] e ROUNDS & NIELSEN [38] desenvolveram métodos de análise por cromatografia líquida por troca iônica que, apesar de serem mais sensíveis e seletivos que o de fase reversa [6], apresentam o inconveniente de utilizar reação pós-coluna, o que torna o método mais caro e complexo.…”

Section: -Introduçãounclassified

Validação de métodos para determinação dos ácidos fítico e oxálico em multimistura

Nappi¹,

Ribeiro-Cunha²,

Coelho³

et al. 2006

Ciênc. Tecnol. Aliment.

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RESUMOConsiderando a composição da multimistura distribuída em Belo Horizonte/MG (trigo, fubá, casca de ovo e folha de mandioca), as metodologias de detecção e/ou quantificação dos fitatos e oxalatos foram validadas. A purificação em coluna de troca aniônica forte do ácido fítico e seus derivados desfosforilados extraídos da multimistura apresentou baixos valores de recuperação (49%) nas concentrações menores do fitato adicionado, em relação às mais elevadas (101%), sugerindo a interferência de minerais presentes em altas concentrações, principalmente cálcio. Dos métodos avaliados para a determinação do ácido oxálico, a cromatografia de exclusão iônica foi a que apresentou os melhores resultados, com boas recuperações nos níveis mais altos de adição (88%), apresentando, contudo, recuperação mais baixa nos níveis inferiores de adição (61%). Os teores de ácido fítico nas amostras variaram de 1,61 g/100 g a 2,25 g/100 g e, para o ácido oxálico, de 0,044 g/100 g a 0,057 g/100 g. Os critérios de validação dos métodos analíticos por cromatografia líquida de alta eficiência foram considerados satisfatórios quanto a seletividade e especificidade, linearidade, repetitividade e limites de detecção e quantificação. Contudo, o de recuperação não foi atendido satisfatoriamente, visto que as porcentagens obtidas foram inferiores ao esperado, levando-se em conta a concentração adicionada nas amostras. Palavras-chave: ácido fítico, acido oxálico, cromatografia, fitatos, complemento alimentar, validação. SUMMARYVALIDATION METHODS TO DETERMINE PHYTIC AND OXALIC ACIDS IN "MULTIMISTURAS". Taking into account the composition of the "multimistura" distributed in Belo Horizonte/MG (wheat, corn meal, egg shell and cassava leave), the methods of detection and/or quantitation of phytates and oxalates were validated. The purification of phytic acid and its dephosphorylated derivatives extracted from the "multimistura" using a strong anionic exchange column presented low values of recovery (49%) at low concentrations of the added phytate, when related to the higher ones (101%), which suggests interference of minerals present in high levels, mainly calcium. From the methods evaluated for the determination of oxalic acid, the ionic exclusion chromatography was the one that presented the best results, with good recoveries at the highest addition levels (88%); presenting, however, decreased recovery in the lower levels of addition (61%). The levels of phytic acid in the samples varied from 1.61 g/100 g to 2.25 g/100 g and for the oxalic acid, from 0.044 g/100 g to 0.057 g/100 g. The validation criteria for the analytical methods by high performance liquid chromatography were considered satisfactory for selectivity and specificity, linearity, repetitivity and limits of detection and quantitation. However, the recovery was not satisfactory since the obtained percentages were lower than the expected one, taking into account the quantities added to the samples. Keywords: oxalic acid, phytic acid, chromatography, phytates, food complement, validati...

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“…The amounts of inositol hexa-, penta-, tetra-and triphosphates (IP 6 , IP 5 , IP 4 and IP 3 ) were determined in diet and fecal samples according to the ion-pair HPLC method of Sandberg and Ahderinne (1986) and modified by Sandberg et al (1989). The isomers of inositol penta-and tetraphosphates in fecal samples were determined employing the method of Skoglund et al (1997).…”

Section: Methodsmentioning

confidence: 99%

“…The sample of Aspergillus niger phytase was incubated during 15 min at 55°C, pH 2.5 and 5.0, respectively, and the sample of wheat phytase was incubated during 1 h at 55°C, pH 5.0. The conditions were chosen with regard to the optimal activity of the enzymes (Peers 1953;Simell et al 1990). The Finase ® FP500 enzyme preparation contains acid phosphatase in addition to phytase.…”

Section: Enzymatic Phytate Degradationsmentioning

confidence: 99%

Phytate hydrolysis in pigs fed a barley-rapeseed meal diet treated with Aspergillus niger phytase or steeped with whey

Skoglund¹,

Näsi²,

Sandberg³

1998

Can. J. Anim. Sci.

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-S. 1998. Phytate hydrolysis in pigs fed a barley-rapeseed meal diet treated with Aspergillus niger phytase or steeped with whey. Can. J. Anim. Sci. 78: 175-180. The degradation of phytate (myo-inositol hexaphosphate) in a barley-rapeseed meal (80:20) diet due to supplemented Aspergillus niger phytase and steeping (soaking at 40°C for 3 h with feed to water ratio 1 kg:1 L) with whey was studied in eight growing pigs (initial weight 27.8 kg). Phytate and its hydrolysis products (inositol penta-, tetra-and triphosphates, abbreviated IP 5 , IP 4 and IP 3 ) in diets and feces were determined using ion-pair high-performance liquid chromatography (HPLC). Different isomeric forms of inositol tetra-and pentaphosphates were studied utilizing high-performance ion chromatography (HPIC). Supplementing the diet with microbial phytase resulted in a 47% reduction in the amount of fecal phytate. Whey steeping of the diet reduced fecal phytate by 35%. Further reduction of the amount of fecal phytate (64%) was demonstrated in pigs fed the diet both steeped with whey and supplemented with microbial phytase, compared with pigs fed the untreated diet. Identification of IP 4 and IP 5 isomers in fecal samples showed which kind of phytase enzyme was active during phytate hydrolysis in the digestive tract. From these data, it was concluded that pigs fed the basal or whey steeped diet, without supplemented microbial phytase, had higher relative fecal amounts of DL-Ins(1,2,3,4,5)P 5 , compared with pigs fed the microbial phytase supplemented diets. Adding microbial phytase to the diet increased the relative amount of DLIns(1,2,4,5,6)P 5 in feces. With whey steeping of the diet, the relative amount of DL-Ins(1,2,3,4)P 4 isomer in feces was increased and the relative amount of DL-Ins(1,2,5,6)P 4 isomer was decreased. . La quantité de phytate dans les fèces était abaissée de 47 % par l'adjonction de phytase microbienne et de 35 % par le trempage dans le petit-lait, par rapport au régime témoin. Une réduction supplémentaire (64 %) était observée chez les porcs recevant l'aliment, à la fois trempé dans le petit-lait et additionné de phytase microbienne. L'identification des isomères IP 4 et IP 5 dans les prélèvements fécaux révélait quelle sorte de phytase était en cause durant l'hydrolyse des phytates dans le tractus digestif. Ces observations nous amènent à conclure que les porcs recevant le régime témoin ou l'aliment trempé dans le petit-lait sans adjonction de phytase microbienne montraient des teneurs fécales relativement plus élevées de DL-Ins (1,2,3,4,5)P 5 que ceux recevant l'aliment additionné de phytase microbienne. En revanche, l'adjonction de phytase accroissait les teneurs relatives de DL-Ins (1,2,4,5,6)P 5 dans les matières fécales. Le trempage dans le petit-lait accroissait les quantités relatives de DL-Ins (1,2,3,4)P 4 dans les fèces et diminuait celles de DL-Ins(1,2,5,6)P 4 . Mots clés: Phosphate d'inositol, trempage, phytase d'Aspergillus niger, porcDiets fed to pigs normally contain high amounts of phosphorus in the form of ...

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Effects of Inositol Tri‐, Tetra‐, Penta‐, and Hexaphosphates on In Vitro Estimation of Iron Availability

Cited by 161 publications

References 19 publications

Hydrothermal treatment and malting of barley improved zinc absorption but not calcium absorption in humans

Hydrothermal treatment and malting of barley improved zinc absorption but not calcium absorption in humans

Validação de métodos para determinação dos ácidos fítico e oxálico em multimistura

Phytate hydrolysis in pigs fed a barley-rapeseed meal diet treated with Aspergillus niger phytase or steeped with whey

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