Effects of Inositol Hexaphosphate (Phytate) on Calcium Binding, Calcium Oxalate Crystallization and In Vitro Stone Growth

Saw, N.K.; Chow, Ka Ming; Rao, P. N.; Kavanagh, John P.

doi:10.1016/j.juro.2007.01.113

Cited by 35 publications

(20 citation statements)

References 19 publications

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“…While IP6 is a known inhibitor of calcium phosphate crystallization, [ 27,28 ] little is known about the inhibitory properties of IP6 on CaOx crystallization. [ 31 ] Thus, in a first step we investigated the effects of IP6 on CaOx crystallization in human urine, as well as the impact of chemical modifications on the in vitro CaOx growth inhibition efficacy of the molecule ( Figure ).…”

Section: Resultsmentioning

confidence: 99%

Inhibitors of Calcium Oxalate Crystallization for the Treatment of Oxalate Nephropathies

et al. 2020

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Calcium oxalate (CaOx) crystal‐induced nephropathies comprise a range of kidney disorders, for which there are no efficient pharmacological treatments. Although CaOx crystallization inhibitors have been suggested as a therapeutic modality already decades ago, limited progress has been made in the discovery of potent molecules with efficacy in animal disease models. Herein, an image‐based machine learning approach to systematically screen chemically modified myo‐inositol hexakisphosphate (IP6) analogues is utilized, which enables the identification of a highly active divalent inositol phosphate molecule. To date, this is the first molecule shown to completely inhibit the crystallization process in the nanomolar range, reduce crystal–cell interactions, thereby preventing CaOx‐induced transcriptomic changes, and decrease renal CaOx deposition and kidney injury in a mouse model of hyperoxaluria. In conclusion, IP6 analogues based on such a scaffold may represent a new treatment option for CaOx nephropathies.

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Section: Resultsmentioning

confidence: 99%

Inhibitors of Calcium Oxalate Crystallization for the Treatment of Oxalate Nephropathies

et al. 2020

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“…Several recent studies reported that PA has beneficial effects on human health through its antioxidant (15), anticancer (16), and antidiabetic (17) effects. It can also protect against the development of Parkinson's disease (18) and renal calculi (19). However, PA has not been widely studied as a natural antimicrobial agent.…”

mentioning

confidence: 99%

Phytic Acid and Sodium Chloride Show Marked Synergistic Bactericidal Effects against Nonadapted and Acid-Adapted Escherichia coli O157:H7 Strains

Kim

Rhee

2016

Appl Environ Microbiol

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The synergistic antimicrobial effects of phytic acid (PA), a natural extract from rice bran, plus sodium chloride against Escherichia coli O157:H7 were examined. Exposure to NaCl alone at concentrations up to 36% (wt/wt) for 5 min did not reduce bacterial populations. The bactericidal effects of PA alone were much greater than those of other organic acids (acetic, citric, lactic, and malic acids) under the same experimental conditions (P < 0.05). Combining PA and NaCl under conditions that yielded negligible effects when each was used alone led to marked synergistic effects. For example, whereas 0.4% PA or 3 or 4% NaCl alone had little or no effect on cell viability, combining the two completely inactivated both nonadapted and acid-adapted cells, reducing their numbers to unrecoverable levels (>7-log CFU/ml reduction). Flow cytometry confirmed that PA disrupted the cell membrane to a greater extent than did other organic acids, although the cells remained viable. The combination of PA and NaCl induced complete disintegration of the cell membrane. By comparison, none of the other organic acids acted synergistically with NaCl, and neither did NaCl-HCl solutions at the same pH values as the test solutions of PA plus NaCl. These results suggest that PA has great potential as an effective bacterial membrane-permeabilizing agent, and we show that the combination is a promising alternative to conventional chemical disinfectants. These findings provide new insight into the utility of natural compounds as novel antimicrobial agents and increase our understanding of the mechanisms underlying the antibacterial activity of PA. Food safety has become an increasingly important aspect of public health. According to the World Health Organization, foodborne and waterborne diarrheal diseases kill an estimated 2 million people per year, many of whom are children (1). Although various chemicals are used to control the transmission of foodborne illnesses via the food and livestock industries (2, 3), several studies have suggested that synthetic sanitizers can have significant side effects (e.g., bleaching, formation of toxic compounds, and off odors) (4, 5). Because modern consumers tend to prefer the use of "natural" agents rather than "synthetic" ones (6), new antimicrobial agents are required; therefore, studies of natural compounds with antimicrobial actions are warranted.Organic acids are a class of such natural antimicrobial agents. Several organic acids (particularly acetic, citric, and lactic acids) have long been used as preservatives or surface sanitizers for beef hides (7), carcasses (8), dairy products (9, 10), fresh-cut vegetables (11), and meat products (12). However, organic acids alone are not powerful enough to inactivate pathogenic bacteria after only a short exposure time (5, 13). Even if this problem could be overcome, applying adequate amounts of organic acids is impractical, because they generate strong acidic odors at working concentrations (5, 13). Thus, it is of vital importance to identify new alternatives ...

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“…The antioxidant activity of phytic acid is being studied in several areas, for example, in food preservation (Stodolak et al, 2007;Canan et al, 2012), oxidation of metals (Gupta et al, 2013), and in human health, with anticancer activity (Norazalina et al, 2010;Norhaizan et al, 2011), in the treatment of diabetes (Lee et al, 2006), renal calculus (Saw et al, 2007), and Parkinson's disease (Xu et al, 2008).…”

Section: Antioxidant Actionmentioning

confidence: 99%

Vinegar: functional aspects

Pazuch¹,

Siepmann²,

Canan³

et al. 2015

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Effects of Inositol Hexaphosphate (Phytate) on Calcium Binding, Calcium Oxalate Crystallization and In Vitro Stone Growth

Cited by 35 publications

References 19 publications

Inhibitors of Calcium Oxalate Crystallization for the Treatment of Oxalate Nephropathies

Inhibitors of Calcium Oxalate Crystallization for the Treatment of Oxalate Nephropathies

Phytic Acid and Sodium Chloride Show Marked Synergistic Bactericidal Effects against Nonadapted and Acid-Adapted Escherichia coli O157:H7 Strains

Vinegar: functional aspects

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