Effects of Inonotus obliquus Polysaccharides on Proliferation, Invasion, Migration, and Apoptosis of Osteosarcoma Cells

Abstract

Objectives. To observe the effect of Inonotus obliquus polysaccharide (IOP) on the proliferation, invasion, migration, and apoptosis of osteosarcoma cells and to elucidate its underlying molecular mechanism. Methods. IOP was extracted from Inonotus obliquus, human osteosarcoma MG-63 cells and U2OS cells were cultured in vitro, and the effects of IOP on the proliferation, migration, invasion, and apoptosis of MG-63 cells and U2OS cells were determined by CCK-8 assays, cell scratch assays, transwell assays, and flow cytometry, respectively. Western blot was used to detect the expression of related proteins in the Akt/mTOR and NF-κB signaling pathways. Results. Compared with the control group, MG-63 cells and U2OS cells treated with IOP of 80 μg/ml, 160 μg/ml, and 320 μ g/ml in the experimental group had significantly lower proliferation activity, decreased migration and invasion ability, and increased apoptosis rate ( P < 0.05 ). Furthermore, IOP could significantly inhibit the activation of the Akt/mTOR and NF-κB signaling pathway ( P < 0.05 ). Conclusion. IOP can regulate the proliferation, migration, invasion, and apoptosis of osteosarcoma cells by inhibiting the activation of the Akt/mTOR signaling pathway. It has antitumor activity on osteosarcoma and has the potential of clinical application in osteosarcoma treatment.

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

“…IOPS on human osteosarcoma MG-63 cells and U2OS cells’ s proliferation, invasion, migration, and apoptosis was studied in vitro by CCK-8 assays, cell scratch assays, trans-well assays, and flow cytometry. Moreover, 320 μg/mL IOPS significantly inhibited proliferation, decreased migration and invasion ability, and increased apoptosis rate ( p < 0.05) as IOPS could significantly inhibit the activation of the Akt/mTOR and NF-κB signaling pathway ( p < 0.05) [ 101 ].…”

In Vitro Immunomodulatory Effects of Inonotus obliquus Extracts on Resting M0 Macrophages and LPS-Induced M1 Macrophages

“…Figure 2 shows that antitumor activities of chaga polysaccharides are achieved through multiple signals including but not limited to the activation of the nod-like receptor family protein 3 (NLRP3) inflammasome, nuclear factor κ appa B(NF- κ B)/mitogen-activated protein kinases (MAPK) pathway, liver kinase B 1(LKB1)/AMPK pathway, and the promoted effects on NO, TNF- α, and IL-1 β level via above signals and immuno-stimulating effects [ 15 – 21 ]. Figure 2 also shows that in vivo hypoglycemic activities of chaga polysaccharides are confirmed by depressing oxidative stress, NF- κ B/transforming growth factor (TGF)- β pathway, and PI3K/Akt pathway.…”

“…In the development of type 2 diabetes mellitus (T2DM), chronic inflammation plays an important role, and the proinflammatory environment maintained by the innate immunity, including macrophages and related cytokines, can be influenced by adaptive immunity [ 7 ]. Our further literature research detailed the relationships between immune-regulatory signaling pathways of chaga polysaccharides and their antidiabetic [ 9 – 14 ] and antitumor activities [ 15 – 21 ] in Figure 2 .…”

Targeting mTOR Signaling by Dietary Polysaccharides in Cancer Prevention: Advances and Challenges

“…This inhibitory effect was mediated by suppressed mTOR signaling. 126 Matrix metalloproteinases (MMPs) degrade extracellular matrix (ECM) proteins, a prerequisite for tumor invasion and metastasis. 127 As a member of the MMP family, MMP-2 could degrade collagen and destroyed the integrity of the ECM.…”

“…In particular, treatment with 320 μg/mL of IOP caused the migratory abilities of the two cell types in scratch experiments to reduce 25% and 18%, respectively. This inhibitory effect was mediated by suppressed mTOR signaling . Matrix metalloproteinases (MMPs) degrade extracellular matrix (ECM) proteins, a prerequisite for tumor invasion and metastasis .…”