Effects of Inoculum Size on Solid-Phase Fermentation of Fodder Beets for Fuel Ethanol Production

Gibbons, William R.; Westby, Carl A.

doi:10.1128/aem.52.4.960-962.1986

Cited by 30 publications

(9 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, inoculation affects the growth of microorganisms and the accumulation of metabolites. Excessive inoculation accelerates the consumption of the medium, whereas low-inoculum cell concentrations prolong the log phase [43,44], both of which reduce the efficiency of fermentation. Besides, culture temperature, rotation speed, and culture time affect the secretion after fermentation in different degrees [45].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Antibacterial Material Production in the Fermentation of Bacillus amyloliquefaciens-9 from Whitespotted Bamboo Shark (Chiloscyllium plagiosum)

Zhang

Wei

et al. 2020

Marine Drugs

View full text Add to dashboard Cite

Bacillus amyloliquefaciens-9 (GBacillus-9), which is isolated from the intestinal tract of the white-spotted bamboo shark (Chiloscyllium plagiosum), can secrete potential antibacterial materials, such as β-1,3-1,4-glucanase and some antimicrobial peptides. However, the low fermentation production has hindered the development of GBacillus-9 as biological additives. In this study, the Plackett-Burman design and response surface methodology were used to optimize the fermentation conditions in a shake flask to obtain a higher yield and antibacterial activity of GBacillus-9. On the basis of the data from medium screening, M9 medium was selected as the basic medium for fermentation. The data from the single-factor experiment showed that sucrose had the highest antibacterial activity among the 10 carbon sources. The Plackett-Burman design identified sucrose, NH 4 Cl, and MgSO 4 as the major variables altering antibacterial activity. The optimal concentrations of these compounds to enhance antibacterial activity were assessed using the central composite design. Data showed that sucrose, NH 4 Cl, and MgSO 4 had the highest antibacterial activities at concentrations of 64.8, 1.84, and 0.08 g L −1 , respectively. The data also showed that the optimal fermentation conditions for the antibacterial material production of GBacillus-9 were as follows: Inoculum volume of 5%, initial pH of 7.0, temperature of 36 • C, rotating speed of 180 rpm, and fermentation time of 10 h. The optimal fermentation medium and conditions achieved to improve the yield of antibacterial materials for GBacillus-9 can enhance the process of developing biological additives derived from GBacillus-9.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of the Antibacterial Material Production in the Fermentation of Bacillus amyloliquefaciens-9 from Whitespotted Bamboo Shark (Chiloscyllium plagiosum)

Zhang

Wei

et al. 2020

Marine Drugs

View full text Add to dashboard Cite

show abstract

“…On one hand, excessive inoculation will speed up the consumption of the medium and lead to the strain being reached in advance in the stationary phase and decline phase. On the other hand, low inoculum volume and cell concentration will extend the logarithmic phase [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Optimization of Fermentation Conditions and Bench-Scale for Improvement of a Novel Glycoprotein GP-1 Production by Streptomyces kanasenisi ZX01

Zhou

et al. 2018

Molecules

View full text Add to dashboard Cite

GP-1 is a novel glycoprotein produced by Streptomyces kanasenisi ZX01 that was isolated from soil near Kanas Lake with significant bioactivity against tobacco mosaic virus. However, extremely low fermentation production has largely hindered further research and market applications on glycoprotein GP-1. In this study, response surface methodology was used to optimize fermentation conditions in a shake flask for higher glycoprotein GP-1 production. When the optimized fermentation conditions were inoculum volume of 6%, initial pH of 6.5, and rotating speed of 150 rpm, glycoprotein GP-1 production could reach 0.9253 mg/L, which was increased by 52.14% compared to the original conditions. In addition, scale-up fermentation was conducted in a 5-L bioreactor to preliminarily explore the feasibility for mass production of glycoprotein GP-1 in a large fermentor, obtaining GP-1 production of 2.54 mg/L under the same conditions, which was 2.75 times higher than the production obtained from a shake flask of 0.9253 mg/L. This work will be helpful to improve GP-1 production on a large scale and lay the foundations for developing it as a novel agent against plant virus.

show abstract

“…Astuti (2003), stated that the competition in the use of substrate effected to the growth of culture becomes poor, because the increasing of cells number or biomass become low. According to Gibbson and Westby (1986), too high inoculum concentrations can lead to a reduction in cell viability. Anggriani et al (2012), adding that too high bacterial populations can lead to the rivalry of the similar types of bacteria (Bacillus sp) and indigenous microorganisms in nutrient or substrate intake that eventually the bacterial activity become inhibited.…”

Section: Percentage Of Oil Degradationmentioning

confidence: 99%

Bioremediation of Spent Bleaching Earth (SBE) Wastes using Lipolitic Bacteria (Bacillus cereus) with Variation of Inoculum Volume

Lusia

Gofar²,

Widjajanti³

2018

STI

View full text Add to dashboard Cite

Spent Bleaching Earth (SBE) is a solid waste was generated from the CPO refining step into cooking oil. SBE that was discharged directly into the environment has the potential to pollute the environment because in the SBE waste contained oil and acid residues, which are easy to be oxidized and flammable. Therefore, SBE must be processed first before being discharged into the environment. One way to manage SBE waste is by bioremediation. Bioremediation is a method of cleaning the environment from contaminants by using biological agents, such as bacteria, fungi, etc. The bacterial isolates used in this study were Bacillus cereus. This study aims to obtain the best inoculum and to know the ability of Bacillus cereus bacteria in degrading the oil content in Spent Bleaching Earth (SBE) waste. This study used a Completely Randomized Design with the volume of Bacillus cereus bacteria inoculum as a treatment, consisting of 6 treatment levels of 0 mL kg-1 , 25 mL kg-1 , 50 mL kg-1 , 75 ml kg-1 , 100 mL kg-1 and 125 mL kg-1. Each treatment level was repeated 3 times so that 18 experimental units were obtained. The observation was done once a week, in a month. Parameters observed were bacterial population, the percentage of oil degradation and oil content after degradation. The best treatment result for the bacterial population was obtained at the treatment of 100 mL kg-1 , at week 4, which was 7.4 x 108 Cfu g-1 , and for the oil, degradation was obtained at 50 ml kg-1 on the treatment at week 4 as big as 90.43%.

show abstract

Effects of Inoculum Size on Solid-Phase Fermentation of Fodder Beets for Fuel Ethanol Production

Cited by 30 publications

References 11 publications

Evaluation of the Antibacterial Material Production in the Fermentation of Bacillus amyloliquefaciens-9 from Whitespotted Bamboo Shark (Chiloscyllium plagiosum)

Evaluation of the Antibacterial Material Production in the Fermentation of Bacillus amyloliquefaciens-9 from Whitespotted Bamboo Shark (Chiloscyllium plagiosum)

Optimization of Fermentation Conditions and Bench-Scale for Improvement of a Novel Glycoprotein GP-1 Production by Streptomyces kanasenisi ZX01

Bioremediation of Spent Bleaching Earth (SBE) Wastes using Lipolitic Bacteria (Bacillus cereus) with Variation of Inoculum Volume

Contact Info

Product

Resources

About