Effects of inner angle of bowl, flow rate and speed on the efficiency of glycerol separation from the raw biodiesel using cylindrical bowl centrifuge

Chungcharoen, Thatchapol; Netjaibun, Kanokphan; Pratabkong, Thaweesak; Suwannasam, Puttapong; Limmun, Warunee

doi:10.1016/j.egypro.2017.10.184

Cited by 5 publications

(2 citation statements)

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“…After determination of temperature optimum, thermal stability Samples for determination of FAME were taken after 1, 2, 3, 6, 12, and 24 hr of synthesis, filtered through a syringe filter (PTFE Syringe filter, pore size: 0.45 μm, i.d. = 25 mm), centrifuged at 2,100 rpm for 5 min, 12 and then diluted with n-heptane by a factor of 100 before the FAME determination.…”

Section: Ph and Temperature Optimum Determinationmentioning

confidence: 99%

Production of biodiesel by Burkholderia cepacia lipase as a function of process parameters

Ostojčić

Budžaki

Flanjak

et al. 2020

Biotechnology Progress

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Despite the already established route of chemically catalyzed transesterification reaction in biodiesel production, due to some of its shortcomings, biocatalysts such as lipases present a vital alternative. Namely, it was noticed that one of the key shortcomings for the optimization of the enzyme catalyzed biodiesel synthesis process is the information on the lipase activity in the reaction mixture. In addition to making optimization difficult, it also makes it impossible to compare the results of the independent research. This article shows how lipase intended for use in biodiesel synthesis can be easily and accurately characterized and what is the enzyme concentration that enables achievement of the desired level of fatty acid methyl esters (FAME) in the final product mixture. Therefore, this study investigated the effect of two different activity loads of Burkholderia cepacia lipase on the biodiesel synthesis varying the pH and temperature optimal for lipase activity. The optimal lipase pH and temperature were determined by two different enzyme assays: spectrophotometric and titrimetric. The B. cepacia lipase pH optimum differentiated between assays, while the lipase optimally hydrolyzed substrates at 50 C. The analysis of FAME during 24 hr of biodiesel synthesis, at two different enzyme concentrations, pH 7, 8, and 10, and using two different buffers, revealed that the transesterification reaction at optimal pH, 1 hr reaction time and lipase activity load of 250 U per gram of reaction mixture was sufficient to produce more than 99% FAME.

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Section: Ph and Temperature Optimum Determinationmentioning

confidence: 99%

Production of biodiesel by Burkholderia cepacia lipase as a function of process parameters

Ostojčić

Budžaki

Flanjak

et al. 2020

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…The biodiesel separation process using centrifuge machines is necessary to understand the interactive effects of various parameters operation, which could influence the separation efficiency and overall biodiesel yield during centrifugation. One key attribute is the interaction between rotation speeds, mixture flow rates, and temperature [ 16 , 17 ]. Previous research has shown that creating relationships between factors can help increase productivity [ 18 ].…”

Section: Introductionmentioning

confidence: 99%