Effects of Inhibitory Activity on Mycelial Growth of &lt;i&gt;Candida albicans&lt;/i&gt; and Therapy for Murine Oral Candidiasis by the Combined Use of Terpinen-4-ol and a Middle-chain Fatty Acid, Capric Acid

Ninomiya, Kentaro; Hayama, Koremasa; Ishijima, Sanae A.; Takahashi, Miki; Kurihara, Junichi; Abe, Shigeru

doi:10.1248/yakushi.12-00242

Cited by 5 publications

(5 citation statements)

References 20 publications

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“…It has been reported that a combination of capric acid and terpinen-4-ol, a major component of tea tree oil, inhibits Candida hyphal growth synergistically (10). We have also reported that oligonol, a low-molecularweight polyphenol formulation derived from lychee fruit, inhibits Candida hyphal growth (11).…”

Section: Inhibition Of C Albicans Hyphal Growth By Saturated Fatty Amentioning

confidence: 88%

Inhibitory effects of several saturated fatty acids and their related fatty alcohols on the growth of Candida albicans

Hayama

Takahashi

Yui

et al. 2015

DD&T

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We examined the effect of 5 saturated fatty acids and their related alcohols on the growth of Candida albicans. The inhibitory effects of these compounds against the yeast and hyphal growth forms of C. albicans were examined using the modified NCCLS method and crystal violet staining, respectively. Among these compounds, capric acid inhibited both types of growth at the lowest concentration. The IC(80), i.e., the concentration at which the compounds reduced the growth of C. albicans by 80% in comparison with the growth of control cells, of capric acid for the hyphal growth of this fungus, which is indispensable for its mucosal invasion, was 16.7 μM. These fatty acids, including capric acid, have an unpleasant smell, which may limit their therapeutic use. To test them at reduced concentrations, the combined effect of these fatty acids and oligonol, a depolymerized polyphenol, was evaluated in vitro. These combinations showed potent synergistic inhibition of hyphal growth [fractional inhibitory concentration (FIC) index = 0.319]. Our results demonstrated that capric acid combined with oligonol could be used as an effective anti-Candida compound. It may be a candidate prophylactic or therapeutic tool against mucosal Candida infection.

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Section: Inhibition Of C Albicans Hyphal Growth By Saturated Fatty Amentioning

confidence: 88%

Inhibitory effects of several saturated fatty acids and their related fatty alcohols on the growth of Candida albicans

Hayama

Takahashi

Yui

et al. 2015

DD&T

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“…Although, the 1,8cineole is the main component of all essential oils (see Table 3 largiflorens has lowest α-pinene (2.3 %) in its oil, therefore, the α-pinene can not be considered as active antifungal compound. Therefore, a direct relationship between terpinen-4-ol content and antifungal 48 . As it is observed from Table 3, the oil of E. largiflorens contains 15.1 % of cryptone and 11.2 % of 4-allyloxyimino-2-caren, while these two compounds were not present in other oils, except for E. oleosa (containing 1.7 % of crypton).…”

Section: The Relationship Between Antifungal and Oils Constituentmentioning

confidence: 99%

The Chemical Composition andin vitroAntifungal Activities of Essential Oils of FiveEucalyptusSpecies

Nikbakht

Rahimi‐Nasrabadi

Ahmadi

et al. 2015

Journal of Essential Oil Bearing Plants

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This study was designed to examine the chemical composition of essential oils of three Eucalyptus spices (E. spathulata, E. microtheca, and E. torquata) and in vitro antifungal activities of the essential oils of five Eucalyptus spices (E. largiflorens, E. oleosa, E. spathulata, E. microtheca, and E. torquata). The chemical composition of the hydrodistilled essential oils of leaves of these three spices were analyzed by GC-MS and GC-FID. The predominant and common components in the all oils were α-pinene (2.3 -14.5 %), 1,8cineole (6.6 -69.6 %), terpinen-4-ol (0.8 -9.6 %), α-terpineol (1.1 -4.3 %), aromadenderene (0.3 -10.5 %) and viridiflorol (0.0 -5.7 %). The antifungal activities of essential oils have been investigated by minimum inhibitory concentration (MIC) and disc diffusion methods against A. flavus, A. parasiticus, A. niger, P. chryzogenum and P. citrinum. A high antifungal activity was found in the leaf oil of E. largiflorens. The relationship between the some major constituents and the antifungal activity was also discussed. We concluded that the terpinen-4-ol may be considered as correspond antifungal constituent.

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“…In preceding papers, we have reported that oral treatment with tea tree oil (TTO) and its main component, terpinen-4-ol (T-4-ol), improved Candida infected lesions on the tongue's surface and lowered the number of Candida cells in the murine oral cavity. 7,12) Interestingly, although TTO and T-4-ol showed an efficacy in oral candidiasis, the recovery of lesion score and the decrease in viable Candida cell number were not correlated 7) ; that led us to assume that there might be several factors affecting the therapeutic results other than the direct anti-Candida efficacy.…”

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confidence: 96%

“…7) Experimental findings in vitro indicated that TTO and/or T-4-ol suppress hyphal growth of C. albicans at low concentrations 12,13) or possess anti-inflammatory activity. 14,15) In this study, we investigated quantitatively: 1. inflammatory responses in murine oral candidiasis, and 2. suppressive efficacy of T-4-ol against the inflammation.…”

mentioning

confidence: 99%

Suppression of Inflammatory Reactions by Terpinen-4-ol, a Main Constituent of Tea Tree Oil, in a Murine Model of Oral Candidiasis and Its Suppressive Activity to Cytokine Production of Macrophages in Vitro

Ninomiya

Hayama

Ishijima

et al. 2013

Biological & Pharmaceutical Bulletin

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The onset of oral candidiasis is accompanied by inflammatory symptoms such as pain in the tongue, edema or tissue damage and lowers the quality of life (QOL) of the patient. In a murine oral candidiasis model, the effects were studied of terpinen-4-ol (T-4-ol), one of the main constituents of tea tree oil, Melaleuca alternifolia, on inflammatory reactions. When immunosuppressed mice were orally infected with Candida albicans, their tongues showed inflammatory symptoms within 24 h after the infection, which was monitored by an increase of myeloperoxidase activity and macrophage inflammatory protein-2 in their tongue homogenates. Oral treatment with 50 µL of 40 mg/mL terpinen-4-ol 3h after the Candida infection clearly suppressed the increase of these inflammatory parameters. In vitro analysis of the effects of terpinen-4-ol on cytokine secretion of macrophages indicated that 800 µg/mL of this substance significantly inhibited the cytokine production of the macrophages cultured in the presence of heat-killed C. albicans cells. Based on these findings, the role of the anti-inflammatory action of T-4-ol in its therapeutic activity against oral candidiasis was discussed.

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Effects of Inhibitory Activity on Mycelial Growth of <i>Candida albicans</i> and Therapy for Murine Oral Candidiasis by the Combined Use of Terpinen-4-ol and a Middle-chain Fatty Acid, Capric Acid

Cited by 5 publications

References 20 publications

Inhibitory effects of several saturated fatty acids and their related fatty alcohols on the growth of <i>Candida albicans </i>

Inhibitory effects of several saturated fatty acids and their related fatty alcohols on the growth of <i>Candida albicans </i>

The Chemical Composition andin vitroAntifungal Activities of Essential Oils of FiveEucalyptusSpecies

Suppression of Inflammatory Reactions by Terpinen-4-ol, a Main Constituent of Tea Tree Oil, in a Murine Model of Oral Candidiasis and Its Suppressive Activity to Cytokine Production of Macrophages <i>in Vitro</i>

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