Effects of Inhibitors on Kinetic Properties of Invertase from Saccharomyces cerevisiae

Sirisatesuwon, Chollada; Ninchan, Boontiwa; Sriroth, Klanarong

doi:10.1007/s12355-019-00757-2

Cited by 6 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Research in the literature has shown that certain metal ions, specifically calcium (Ca 2+ ), potassium (K + ), and magnesium (Mg 2+ ), have an effect on the activity of the invertase enzyme. , M1 is a YEPD medium. M2 was prepared as a comparison medium to distinguish the effect of the presence of metal ions from the effect of using fruit pulp.…”

Section: Resultsmentioning

confidence: 99%

“…The activity of many enzymes, including invertase, can be affected by the presence of specific metal ions. In particular, the activity of invertase may be influenced by calcium ions (Ca 2+ ) and magnesium ions (Mg 2+ ) . These ions have the potential to interact with the enzyme, possibly altering its structure or its interaction with the substrate, leading to changes in the enzyme’s activity.…”

Section: Resultsmentioning

confidence: 99%

“…However, the precise effect can vary and would necessitate experimental determination. It is also crucial to note that while certain metal ions can enhance enzyme activity, others may inhibit it …”

Section: Resultsmentioning

confidence: 99%

“…In particular, the activity of invertase may be influenced by calcium ions (Ca 2+ ) and magnesium ions (Mg 2+ ). 31 These ions have the potential to interact with the enzyme, possibly altering its structure or its interaction with the substrate, leading to changes in the enzyme's activity. However, the precise effect can vary and would necessitate experimental determination.…”

Section: Determination Of the Kinetic Constantmentioning

confidence: 99%

“…It is also crucial to note that while certain metal ions can enhance enzyme activity, others may inhibit it. 31 The impact of different metal ions on the immobilized invertase was examined in this study. Results indicated that Co 2+ ions exhibited the inhibition of immobilized enzyme activity, while other metal ions showed slightly varying degrees of inhibitory effects (shown in Figure 8B).…”

Section: Determination Of the Kinetic Constantmentioning

confidence: 99%

See 4 more Smart Citations

Industrial Approach to Invertase Production from Fruit Waste for Enhanced Efficiency and Conservation

Dokuzparmak

2024

ACS Omega

View full text Add to dashboard Cite

This study investigates the commercial viability of repurposing fruit waste for enzyme production, specifically focusing on the invertase enzyme derived from Saccharomyces cerevisiae. By utilizing fruit pulp that incorporates mulberry, carob, Figure, and grape pulp as a nutrient source, it is observed that the culture medium containing carob pulp exhibits the highest invertase activity. Specifically, the invertase activity in this medium is approximately 2.5 times greater (12.90 U/mg protein) than that observed in the peptone medium (5.98 U/mg protein). The extract undergoes several purification steps, including ultrafiltration, ammonium sulfate precipitation, dialysis, and ion-exchange chromatography (purification ratio: 12.11 times, yield: 26.93%). The purified enzyme is immobilized using alginate beads, improving pH and thermal stability. The immobilized enzyme exhibits optimal activity between pH 3.50 and pH 7.00, thereby broadening the enzyme's high-activity pH range. The thermal stability of the immobilized invertase enzyme is significantly improved, especially at 65 °C. Activity studies in the presence of metal ions and certain chemicals have been conducted. The immobilized enzyme's activity increases by approximately 40% in the presence of Ca 2+ and Mg 2+ , and the immobilized enzyme maintains its activity in the presence of detergents such as SDS, Tween-20, and organic solvents like ethanol and methanol. The potential for the reuse of immobilized invertase was investigated under standard assay conditions. After 20 cycles, the immobilized enzyme was found to retain 80% of its initial activity. Overall, the study establishes the commercial potential of fruit pulp, typically discarded in fruit juice production, as a valuable source for obtaining an invertase enzyme. Furthermore, this study also aims to develop a suitable purification process for invertase in the fruit juice industry. By harnessing fruit waste and implementing innovative enzyme production strategies, industries can enhance their efficiency, reduce their environmental footprint, and optimize resource utilization.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Determination Of the Kinetic Constantmentioning

confidence: 99%

Section: Determination Of the Kinetic Constantmentioning

confidence: 99%

See 3 more Smart Citations