Effects of Inhaled Oxidant and Acidic Air Pollutant Combinations on Nasal and Tracheal Tissues in Exercising Rats

Kleinman, Michael T.; McClure, Thomas R.; Mautz, William J.; Phalen, Robert F.; Crocker, T. Timothy

doi:10.1016/b978-0-08-034185-9.50029-2

Cited by 2 publications

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References 7 publications

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“…The work reported here is particularly relevant to inhalation of ozone and its effects on pulmonary surfactant in the alveolar region of the lung. Thus, ozone is known to have a variety of effects (53,54), including changes in chemical composition of lung lavage fluids (8,10,15,(55)(56)(57)(58) and damage to the underlying cells (59)(60)(61)(62)(63), as well as induction of an inflammatory response which lasts several days after exposure (61,62). It is possible that at least some of these effects are initiated by free-radical reactions in the liquid lining of the alveolar region which are induced by the decomposition of the secondary ozonides.…”

Section: Discussionmentioning

confidence: 99%

Formation of secondary ozonides from the reaction of an unsaturated phosphatidylcholine with ozone

Finlayson-Pitts²,

Wv³

1990

Chem. Res. Toxicol.

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Phosphatidylcholines are significant components of pulmonary surfactant in the alveolar region of the lung, where they play a major role in lung function due to their surface tension reducing properties. However, separation and the direct identification of many of the primary products of reaction of phosphatidylcholines with inhaled pollutant gases has not been possible until recently due to the lack of suitable analytical techniques, so that compounds such as fatty acid methyl esters generally have been used as analogues for the phospholipids. We report here the first isolation and identification of the products of reaction of ozone with one of the unsaturated components of lung surfactant, beta-oleoyl-gamma-palmitoyl-L-alpha-phosphatidylcholine (OPPC), using a combination of high-performance liquid chromatography, fast atom bombardment mass spectrometry, and Fourier transform infrared, ultraviolet absorption, and nuclear magnetic resonance spectrometry as well as gas chromatography. The products are shown to be the cis and trans secondary ozonides of the parent phosphatidylcholine, analogous to those previously observed by other researchers in the reactions of the simple fatty acid methyl esters with ozone. This also appears to be the first report of fast atom bombardment mass spectra of these phospholipid secondary ozonides. The implications of this work for the inhalation of ozone, formed in photochemical smog, are discussed.

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