Effects of inhaled air pollution on markers of integrity, inflammation, and microbiota profiles of the intestines in Apolipoprotein E knockout mice

Fitch, Megan N.; Phillippi, Danielle; Zhang, Yan; Lucero, Jo Ann; Pandey, Ravi S.; Liu, June; Brower, Jeremy; Allen, Michael S.; Campen, Matthew J.; McDonald, Jacob D.; Lund, Amie K.

doi:10.1016/j.envres.2019.108913

Cited by 40 publications

(16 citation statements)

References 71 publications

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“…after DEP exposure. While intestinal inflammation and injury, and modified gut permeability have been reported after PM administration [ 22 , 25 – 27 , 32 , 33 ], our study did not reveal overt intestinal toxicity, based on histopathological analysis. We cannot, however, exclude subtler intestinal toxicity because we did not assess immunological cells and mediators, intestinal inflammation or permeability.…”

Section: Discussioncontrasting

confidence: 64%

“…and IL-10 −/− 129/Sv standard gavage urban PM 10 18 μg/g b.w./d 10–13 μg/g/d 7–14 d 35 d + + Salim et al 2014 [ 26 ] IL-10 −/− 129/Sv, F standard food urban PM 10 9 μg/g food 10–20 wk + + Li et al 2017 [ 27 ] Ldlr −/− C57Bl/6, M Western diet gavage UFP 3 × 40 μg/wk + Xu et al 2017 [ 31 ] w.t. and nrf2 −/− C57Bl/6, M standard inhalation PM 2.5 64 μg/m 3 12 wk + + Mutlu et al 2018 [ 22 ] C57Bl/6, M standard inhalation PM 135 μg/m 3 3 wk + Wang et al 2018 [ 23 ] C57Bl/6j, M standard inhalation PM 2.5 276 ± 170 μg/m 3 1 y + + Li et al 2019 [ 32 ] C57Bl/6, MF standard inhalation DEP 300 μg/m 3 , 1 h/d 28 d + + Fitch et al 2020 [ 33 ] ApoE −/− , M high fat ...…”

Section: Discussionmentioning

confidence: 99%

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Diesel exhaust particles alter the profile and function of the gut microbiota upon subchronic oral administration in mice

et al. 2021

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Background Ambient air pollution by particulate matters, including diesel exhaust particles (DEP), is a major cause of cardiovascular and metabolic mortality worldwide. The mechanisms by which DEP cause these adverse outcomes are not completely understood. Because the gut microbiota controls cardiovascular and metabolic health, we hypothesized that the fraction of inhaled DEP which reach the gut after mucociliary clearance and swallowing might induce gut dysbiosis and, in turn, contribute to aggravate or induce cardiovascular and metabolic diseases. Results Female ApoE−/− mice fed a Western diet, and wild-type (C57Bl/6) mice fed standard diet were gavaged with DEP (SRM2975) doses corresponding to mucociliary clearance from inhalation exposure (200 or 1000 ng/day, 3 times a week for 3 months; and 40, 200 or 1000 ng/day, 3 times a week for 6 months, respectively). No mortality, overt systemic or digestive toxicity was observed. A dose-dependent alteration of the gut microbiota was recorded in both strains. In ApoE−/−, β-diversity was modified by DEP, but no significant modification of the relative abundance of the phyla, families or genera was identified. In C57BL/6 mice, DEP reduced α-diversity (Shannon and Simpson indices), and modified β-diversity, including a reduction of the Proteobacteria and Patescibacteria phyla, and an increase of the Campylobacterota phylum. In both mouse models, perturbation of the gut microbiota composition was associated with a dose-dependent reduction of bacterial short chain fatty acids (butyrate and propionate) in cecal content. However, DEP ingestion did not aggravate (ApoE−/−), or induce (C57BL/6 mice) atherosclerotic plaques, and no metabolic alteration (glucose tolerance, resistance to insulin, or lipidemia) was recorded. Conclusions We show here that oral exposure to DEP, at doses relevant for human health, changes the composition and function of the gut microbiota. These modifications were, however, not translated into ultimate atherosclerotic or metabolic outcomes.

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Diesel exhaust particles alter the profile and function of the gut microbiota upon subchronic oral administration in mice

et al. 2021

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“…Fine particulate matter has become one hotspot in china because of its bad air quality [33]. The related mechanism also is explored, and more studies have been carried out about inflammation [34]. As for the study design, there is also one obvious transition from time series to cohort.…”

Section: Burst Abstractmentioning

confidence: 99%

A Bibliometric Analysis of the Impacts of Air Pollution on Children

Sun

Zhou

Huang

et al. 2020

IJERPH

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In recent years, many researchers have investigated the association between air pollution and children. However, there has been little research to provide a macroscopic overview in this field. The aim of this study is to characterize the scientific production around the world in this area and map the trends. The relevant literature was searched from 1999 to 2018. To guarantee the quality of the literature, we combined the PubMed and WoS databases. The built-in statistics tools of the Web of Science website were used to display the trend of articles published by year and the distribution of journals. By CiteSpace (5.5.R2), the reference co-citation and burst keywords were extracted. In total, 15,999 target English documents were obtained. We summarized the characteristics of published documents, of research institutes’ cooperation, and of the contents. As part of a research hotspot, ten clusters are presented, four popular topics are elaborated. Twenty-four burst words were obtained and analyzed. China has received more attention in recent years. Researchers in this field could carry out more cohorts’ studies and fine particulate matter is one good air pollution index. Household air pollution exposure and children’s lung function should be paid more attention.

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“…The human microbiome is understood to be influenced by several factors, including diet and environmental exposures ( Tasnim et al, 2017 ). There are a few emerging studies that show inhaled air pollutants from both anthropogenic and natural sources can also induce alteration in the Firmicutes: Bacteroidetes ratio in the GI tract, which increases susceptibility to inflammation ( Fitch et al, 2020 ; Mutlu et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Traffic generated emissions alter the lung microbiota by promoting the expansion of Proteobacteria in C57Bl/6 mice placed on a high-fat diet

Daniel

Pusadkar

McDonald

et al. 2021

Ecotoxicology and Environmental Safety

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Air pollution has been documented to contribute to severe respiratory diseases like asthma and chronic obstructive pulmonary disorder (COPD). Although these diseases demonstrate a shift in the lung microbiota towards Proteobacteria, the effects of traffic generated emissions on lung microbiota profiles have not been well-characterized. Thus, we investigated the hypothesis that exposure to traffic-generated emissions can alter lung microbiota and immune defenses. Since a large population of the Western world consumes a diet rich in fats, we sought to investigate the synergistic effects of mixed vehicle emissions and high-fat diet consumption. We exposed 3-month-old male C57Bl/6 mice placed either on regular chow (LF) or a high-fat (HF: 45% kcal fat) diet to mixed emissions (ME: 30 μg PM/m 3 gasoline engine emissions + 70 μg PM/m 3 diesel engine emissions) or filtered air (FA) for 6 h/d, 7 d/wk for 30 days. Levels of pulmonary immunoglobulins IgA, IgG, and IgM were analyzed by ELISA, and lung microbial profiling was done using qPCR and Illumina 16 S sequencing. We observed a significant decrease in lung IgA in the ME-exposed animals, compared to the FA-exposed animals, both fed a HF diet. Our results also revealed a significant decrease in lung IgG in the ME-exposed animals both on the LF diet and HF diet, in comparison to the FA-exposed animals. We also observed an expansion of Enterobacteriaceae belonging to the Proteobacteria phylum in the ME-exposed groups on the HF diet. Collectively, we show that the combined effects of ME and HF diet result in decreased immune surveillance and lung bacterial dysbiosis, which is of significance in lung diseases.

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Effects of inhaled air pollution on markers of integrity, inflammation, and microbiota profiles of the intestines in Apolipoprotein E knockout mice

Cited by 40 publications

References 71 publications

Diesel exhaust particles alter the profile and function of the gut microbiota upon subchronic oral administration in mice

Diesel exhaust particles alter the profile and function of the gut microbiota upon subchronic oral administration in mice

A Bibliometric Analysis of the Impacts of Air Pollution on Children

Traffic generated emissions alter the lung microbiota by promoting the expansion of Proteobacteria in C57Bl/6 mice placed on a high-fat diet

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