2020
DOI: 10.3390/app10082685
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Effects of Infusion and Storage on Antioxidant Activity and Total Phenolic Content of Black Tea

Abstract: This study determined antioxidant activity in terms of the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging ability and total phenolic content of black tea under different infusion and storage conditions. High performance liquid chromatography analysis identified caffeine, (−)-epigallocatechin, (−)-epicatechin-3-gallate, (−)-epigallocatechin-3-gallate and (−)-gallocatechin-3-gallate in the tea sample. The water–tea leaves weight ratio did not affect the DPPH scavenging ability. However, infusion temperature aff… Show more

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Cited by 38 publications
(20 citation statements)
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“…DPPH scavenging activity was measured spectrophotometrically according to the method reported in previous papers [27][28][29][30][31][32]. The bitter gourd sample (2 mL juice sample) and the 0.08 mg/mL DPPH solution prepared with methanol (2 mL) were mixed together.…”
Section: 1-diphenyl-2-picrylhydrazyl Scavenging Ability Assaymentioning
confidence: 99%
See 1 more Smart Citation
“…DPPH scavenging activity was measured spectrophotometrically according to the method reported in previous papers [27][28][29][30][31][32]. The bitter gourd sample (2 mL juice sample) and the 0.08 mg/mL DPPH solution prepared with methanol (2 mL) were mixed together.…”
Section: 1-diphenyl-2-picrylhydrazyl Scavenging Ability Assaymentioning
confidence: 99%
“…TPC was determined according to the Folin-Ciocalteu colorimetric assay following the method described in previous reports [28][29][30][31][32][33]. The bitter gourd sample (1 mL juice sample) was added to 1 mL of 1N Folin-Ciocalteu reagent and 0.8 mL of 7.5% Na 2 CO 3 solution.…”
Section: Determination Of Total Phenolic Contentmentioning
confidence: 99%
“…Black tea extract owes its antioxidant properties mainly to the presence of polyphenols [ 64 ]. Its concentration depends on many factors related to the processing of the leaves but also to the process of their infusion including the pH of the water used, the temperature, and the infusion time [ 65 ]. In this study, the antioxidant activity of black tea extract was calculated as 453.70 mM Trolox based on the ABTS assay.…”
Section: Resultsmentioning
confidence: 99%
“…The absorbance of the mixture is measured at 760 nm in triplicate for 30 min. Gallic acid as a standard and total Phenolic Content is expressed as an equivalent of Gallic acid per gram of extract 24 .…”
Section: Quantitative Estimation Of Phytochemicalsmentioning
confidence: 99%