Effects of infrasound at 8ï¿½Hz 90ï¿½dB/130ï¿½dB on NMDAR1 expression and changes in intracellular calcium ion concentration in the hippocampus of rats

Liu, Zhaohui; Chen, Jingzao; Lin, Yuanyuan; Liu, Jing; Qiu, Jian; Xu, Jian; Ling, Rui; Yuan, Xiao-Chao; Zhang, Wendong; Li, Xiaofang; Gong, Li

doi:10.3892/mmr.2010.369

Cited by 6 publications

(6 citation statements)

References 9 publications

(14 reference statements)

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“…Accumulating evidence has shown that infrasonic exposure induces abnormal ultrastructures in cortical and hippocampal neurons (6,7) and promotes blood-brain barrier permeability (5), leading to severe damage to brain tissue (16 ] i in hippocampal neurons increased following infrasonic exposure; it peaked on day 14 (90 dB), and gradually decreased to normal levels after 28 days of infrasonic exposure, suggesting a self-repairing capability occurred in the cells. A similar time-dependent pattern has also been observed in hippocampal neuron injury as described in previous studies (4,6). It has been shown that the increased expressions of 70-kDa heat-shock protein (Hsp70) and glial fibrillary acidic protein are detected following a period of infrasonic exposure, suggesting the critical role of ) at (A) 14 or (B) 28 days after infrasonic exposure was quantified by staining with Annexin V and PI.…”

Section: Discussionsupporting

confidence: 65%

“…Accumulating evidence has demonstrated that infrasound of a certain sound pressure level (SPL), usually given in decibels (dB), may lead to dysfunction of the human body (2). Moreover, exposure to infrasound has been shown to induce damage to the central nervous system (CNS) in animal models (3)(4)(5)(6). However, the underlying mechanism remains to be clarified.…”

Section: Introductionmentioning

confidence: 99%

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Infrasound increases intracellular calcium concentration and induces apoptosis in hippocampi of adult rats

Liu

2011

Mol Med Report

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Abstract. In the present study, we determined the effect of infrasonic exposure on apoptosis and intracellular free Ca 2+ ([Ca 2+ ] i ) levels in the hippocampus of adult rats. Adult rats were randomly divided into the control and infrasound exposure groups. For infrasound treatment, animals received infrasonic exposure at 90 (8 Hz) or 130 dB (8 Hz) for 2 h per day. Hippocampi were dissected, and isolated hippocampal neurons were cultured. The [Ca 2+ ] i levels in hippocampal neurons from adult rat brains were determined by Fluo-3/ AM staining with a confocal microscope system on days 1, 7, 14, 21 and 28 following infrasonic exposure. Apoptosis was evaluated by Annexin V-FITC and propidium iodide double staining. Positive cells were sorted and analyzed by flow cytometry. Elevated [Ca 2+ ] i levels were observed on days 14 and 21 after rats received daily treatment with 90 or 130 dB sound pressure level (SPL) infrasonic exposure (p<0.01 vs. control). The highest levels of [Ca 2+ ] i were detected in the 130 dB SPL infrasonic exposure group. Meanwhile, apoptosis in hippocampal neurons was found to increase on day 7 following 90 dB SPL infrasound exposure, and significantly increased on day 14. Upon 130 dB infrasound treatment, apoptosis was first observed on day 14, whereas the number of apoptotic cells gradually decreased thereafter. Additionally, a marked correlation between cell apoptosis and [Ca 2+ ] i levels was found on day 14 and 21 following daily treatment with 90 and 130 dB SPL, respectively. These results demonstrate that a period of infrasonic exposure induced apoptosis and upregulated [Ca 2+ ] i levels in hippocampal neurons, suggesting that infrasound may cause damage to the central nervous system (CNS) through the Ca 2+ -mediated apoptotic pathway in hippocampal neurons.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Infrasound increases intracellular calcium concentration and induces apoptosis in hippocampi of adult rats

Liu

2011

Mol Med Report

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“…In the current study, the cardiac myocytes were exposed to infrasound at 130 dB for up to 72 h. Whereas, Bao et al [20] exposed B lymphoma Raji cells to infrasound at 90 dB for only 120 min. Experimental studies have been reported that humans or various species of animals, such as rats and mice, exposed to infrasound at 90 dB and higher for short or long terms up to several months exhibited significant toxicological effects [8,21]. Why we currently used infrasound at 130 dB with the duration up to 72 h is based on the following reasons: (1) Previously, we found infrasound exposure at 130 dB significantly dysregulated the hemodynamics [8], ultrastructure [8], and L-type calcium currents [9] of cardiac myocytes and sexual behavior [5] in rats.…”

Section: Discussionmentioning

confidence: 99%

Infrasound Exposure Induces Apoptosis of Rat Cardiac Myocytes by Regulating the Expression of Apoptosis-Related Proteins

et al. 2011

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It has been reported that exposure to infrasound causes cardiac dysfunction. Allowing for the key role of apoptosis in the pathogenesis of cardiovascular diseases, the objective of this study was to investigate the apoptotic effects of infrasound. Cardiac myocytes cultured from neonatal rats were exposed to infrasound of 5 Hz at 130 dB. The apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling. Also, the expression levels of a series of apoptosis-related proteins were detected. As a result, infrasound induced apoptosis of cultured rat cardiac myocytes in a time-dependant manner. The expression of proapoptotic proteins such as Bax, caspase-3, caspase-8, caspase-9, and FAS was significantly up-regulated, with concomitant down-regulated expression of antiapoptotic proteins such as Bcl-x, and the inhibitory apoptosis proteins family proteins including XIAP, cIAP-1, and cIAP-2. The expression of poly (ADP-ribose) polymerase and β-catenin, which are the substrate proteins of caspase-3, was significantly decreased. In conclusion, infrasound is an apoptotic inducer of cardiac myocytes.

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“…These adverse influences can also cause diencephalic hypothalamic syndrome with sensor-somatic and autonomic visceral symptoms [35][36][37][38][39][40]. -adverse influence on the functions of the gastrointestinal organs, spleen and liver [41,42,43], -cause visual impairment [44]; -affects hormonal equilibrium [34,45].…”

Section: Results and Disscussionmentioning

confidence: 99%

Exposure to infrasonic noise in agriculture

Bilski¹

2017

Ann Agric Environ Med.

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Introduction and objectives. Although exposure to audible noise has been examined in many publications, the sources of infrasound in agriculture have not been fully examined and presented. The study presents the assessment of exposure to infrasound from many sources at workplaces in agriculture with examples of possible ergonomic and health consequences caused by such exposure. Materials and method. Workers'-perceived infrasonic noise levels were examined for 118 examples of moving and stationary agricultural machines (modern and old cab-type tractors, old tractors without cabins, small tractors, grinders, chargers, forage mixers, grain cleaners, conveyors, bark sorters and combine-harvesters). Measurements of infrasound were taken with the use of class 1 instruments (digital sound analyzer DSA-50 digital and acoustic calibrator). Noise level measurements were performed in accordance with PN-Z-01338:2010, PN-EN ISO 9612:2011 and ISO 9612:2009. Results and conclusions. The most intense sources of infrasound in the study were modern and old large size types agricultural machinery (tractors, chargers and combined-harvesters, and stationary forage mixers with ventilation). The G-weighted infrasound levels were significant and at many analyzed workplaces stayed within or exceeded the occupational exposure limit (L G eq, 8h = 102 dB) when the duration of exposure is longer than 22 min./8-hours working day (most noisy -modern cab-type tractors), 46 min./8 hours working day (most noisy -old type cab-tractors), 73 min./8 hours working day (most noisy -old tractors without cabins), 86 min./8-hours working day (most noisy -combine-harvesters) and 156 min./8 hours working day (most noisy -stationary forage mixers with ventilation). All measured machines generated infrasonic noise exceeded the value L G eq, Te = 86 dB (occupational exposure limit for workplaces requiring maintained mental concentration). A very important harmful factor is infrasound exposure for pregnant women and adolescents at workplaces in agriculture. Very valuable work can be technical limiting exposure to infrasound from new and used agricultural machinery. The technical limitation of infrasound caused by both old and new agricultural machinery can be invaluable from the work point of view.

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Effects of infrasound at 8ï¿½Hz 90ï¿½dB/130ï¿½dB on NMDAR1 expression and changes in intracellular calcium ion concentration in the hippocampus of rats

Cited by 6 publications

References 9 publications

Infrasound increases intracellular calcium concentration and induces apoptosis in hippocampi of adult rats

Infrasound increases intracellular calcium concentration and induces apoptosis in hippocampi of adult rats

Infrasound Exposure Induces Apoptosis of Rat Cardiac Myocytes by Regulating the Expression of Apoptosis-Related Proteins

Exposure to infrasonic noise in agriculture

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