Effects of industrial effluents containing moderate levels of antibiotic mixtures on the abundance of antibiotic resistance genes and bacterial community composition in exposed creek sediments

Milaković, Milena; Vestergaard, Gisle; González-Plaza, Juan José; Petrić, Ines; Kosić-Vukšić, Josipa; Senta, Ivan; Kublik, Susanne; Schloter, Michael; Udiković-Kolić, Nikolina

doi:10.1016/j.scitotenv.2019.136001

Cited by 24 publications

(13 citation statements)

References 75 publications

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“…Although one way ANOVA analysis was not performed in our meta-analysis, we found that the abundance of ARGs in HWW was higher than that in municipal wastewater systems, but no such differences were found in the comparison between HWW and pharmaceutical manufacturing wastewater or livestock wastewater (16). For example, the abundance of ermB in azithromycin-manufacturing wastewaters was higher than that in HWW for a high density of azithromycin residues (46)(47)(48). Meanwhile, the abundance of sul1, sul2, tetO, and tetW was higher but the abundance of bla NDM was lower in livestock wastewater, compared with that in HWW (49,50).…”

Section: Discussionmentioning

confidence: 98%

Hospital Wastewater as a Reservoir for Antibiotic Resistance Genes: A Meta-Analysis

Zhang

Huang

Zhao

et al. 2020

Front. Public Health

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Background: The emergence and dissemination of antibiotic resistance genes (ARGs) in the environment poses a huge global health hazard. Hospital wastewater (HWW), in which a high density of antibiotic residues and antibiotic-resistant bacteria are present, may be a reservoir of ARGs dissemination into the environment. Our meta-analysis comprehensively analyzes the prevalence of ARGs in HWW, as well as the influencing factors in ARGs distribution.Methods: Online databases were used to search for literature using the subject terms: "Drug Resistance" AND "Genes" AND "Hospitals" AND "Wastewater." Two reviewers independently applied predefined criteria to assess the literature and extract data including "relative abundance of ARGs," "title," "authors," "country," "location," "sampling year," and "sampling seasons." The median values and 95% confidence intervals of ARGs abundance were calculated by Wilcox.test function in R. Temporal trends, spatial differences, seasonal variations and removal efficiency of ARGs were analyzed by Pearson correlation analysis and Kruskal-Wallis H test.Results: Resistance genes to carbapenems, sulfonamides, tetracyclines and mobile genetic elements were found at high relative abundance (>10 −4 gene copies/16S rRNA gene copies) in HWW. The abundance of resistance genes to extended-spectrum β-lactams, carbapenems, sulfonamides and glycopeptide significantly decreased, while tetracycline resistance genes abundance increased from 2014 to 2018. The abundance of ARGs was significantly different by country but not by season. ARGs could not be completely removed by on-site HWW treatments and the removal efficiency varies for different ARGs.Conclusions: HWW presents more types of ARGs, and their abundance is higher than those in most wastewater systems. HWW may be a reservoir of ARGs and play an important role in the dissemination of ARGs.

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Section: Discussionmentioning

confidence: 98%

Hospital Wastewater as a Reservoir for Antibiotic Resistance Genes: A Meta-Analysis

Zhang

Huang

Zhao

et al. 2020

Front. Public Health

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“…As shown in Figure 4, the pair peaks of flavins which mediated MIET (flavin-MIET) and c-Cyts which mediated DIET (c-Cyts-DIET) were detected and identified by the pure flavins and c-Cyts in the SRB-SOB coculture (Figure S3a). The peaks of flavin-MIET negatively shifted from −0.480/−0.508 V (E pa /E pc , vs Ag/AgCl) (SRB) and −0.490/−0.572 V (SOB) to −0.535/−0.532 V, along with the corresponding anodic peak currents 42 increasing from −0.175 (SRB) and −0.040 μA (SOB) to 0.240 μA. Meanwhile, the discrepancy ΔE p (E pa − E pc ) of flavins decreased from 0.028 (SRB) and 0.082 (SOB) to 0.003 V.…”

Section: Verification Of Interspecific Electron Transfer (Iet) Betwee...mentioning

confidence: 98%

Interspecific Electron Transfer-Driven Oxytetracycline Degradation by Autotrophic Coculture of Sulfur-Oxidizing and Sulfate-Reducing Bacteria

Qian

Zhang

et al. 2023

ACS EST Water

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Interspecific electron transport (IET) can promote the cometabolism of microorganisms, so it is of great significance in environmental remediation. Herein, a coculture of autotrophic sulfur-oxidizing bacteria (SOB) and heterotrophic sulfate-reducing bacteria (SRB) was constructed to biodegrade oxytetracycline (OTC) (upgraded to 120.9 ± 3.9 μg mg–1 protein d–1) under inorganic conditions, and the biodegradation was markedly improved (1.5 times) after loading biosynthetic FeS (bio-FeS). The increase of the NAD+/NADH ratio and ATPase activity indicated that more electrons generated by intracellular metabolism inside the SOB (lacking the OTC enzyme) outflowed extracellularly to the SRB via the IET chain in the coculture system. Linear sweep voltammetry (LSV) and I–t analysis indicated that bio-FeS on the SRB could enhance direct interspecific electron transport (DIET) mainly via c-Cyts, together with the mediated interspecific electron transport (MIET) via flavins, thus accelerating the OTC efflux. Community analysis demonstrated that the SRB introduction increased the abundance of genes related to environmental information, cell motility, membrane transport, and signal transduction in the coculture system. This discovery revealed the feasibility of antibiotic degradation using heterotrophic bacteria (such as SRB) under inorganic conditions and deepened the understanding of the antibiotic degradation in biogeochemical cycles involving carbon, nitrogen, and sulfur in natural ecosystems.

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“…framework, criterion 1 emphasises the importance of human‐impacted environments that can receive inputs of human or animal faeces, therefore mixing ARGs enriched in humans or farm animals, and their associated MGEs with the environmental bacteria (Karkman et al., 2019; Peters and Zitomer, 2021). Furthermore, ARGs can be enriched in environments that are polluted by antimicrobials or other selective or co‐selective chemicals such as effluents from pharmaceutical manufacturing, mining or smelting (Milaković et al., 2020; Corella et al., 2021).…”

Section: Assessmentmentioning

confidence: 99%

Role played by the environment in the emergence and spread of antimicrobial resistance (AMR) through the food chain

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2021

EFS2

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The role of food-producing environments in the emergence and spread of antimicrobial resistance (AMR) in EU plant-based food production, terrestrial animals (poultry, cattle and pigs) and aquaculture was assessed. Among the various sources and transmission routes identified, fertilisers of faecal origin, irrigation and surface water for plant-based food and water for aquaculture were considered of major importance. For terrestrial animal production, potential sources consist of feed, humans, water, air/dust, soil, wildlife, rodents, arthropods and equipment. Among those, evidence was found for introduction with feed and humans, for the other sources, the importance could not be assessed. Several ARB of highest priority for public health, such as carbapenem or extended-spectrum cephalosporin and/or fluoroquinolone-resistant Enterobacterales (including Salmonella enterica), fluoroquinolone-resistant Campylobacter spp., methicillin-resistant Staphylococcus aureus and glycopeptide-resistant Enterococcus faecium and E. faecalis were identified. Among highest priority ARGs bla CTX-M , bla VIM , bla NDM , bla OXA-48like , bla OXA-23 , mcr, armA, vanA, cfr and optrA were reported. These highest priority bacteria and genes were identified in different sources, at primary and post-harvest level, particularly faeces/manure, soil and water. For all sectors, reducing the occurrence of faecal microbial contamination of fertilisers, water, feed and the production environment and minimising persistence/recycling of ARB within animal production facilities is a priority. Proper implementation of good hygiene practices, biosecurity and food safety management systems is very important. Potential AMR-specific interventions are in the early stages of development. Many data gaps relating to sources and relevance of transmission routes, diversity of ARB and ARGs, effectiveness of mitigation measures were identified. Representative epidemiological and attribution studies on AMR and its effective control in food production environments at EU level, linked to One Health and environmental initiatives, are urgently required.

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Effects of industrial effluents containing moderate levels of antibiotic mixtures on the abundance of antibiotic resistance genes and bacterial community composition in exposed creek sediments

Cited by 24 publications

References 75 publications

Hospital Wastewater as a Reservoir for Antibiotic Resistance Genes: A Meta-Analysis

Hospital Wastewater as a Reservoir for Antibiotic Resistance Genes: A Meta-Analysis

Interspecific Electron Transfer-Driven Oxytetracycline Degradation by Autotrophic Coculture of Sulfur-Oxidizing and Sulfate-Reducing Bacteria

Role played by the environment in the emergence and spread of antimicrobial resistance (AMR) through the food chain

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