Effects of indole alkaloids from leaf of Alstonia scholaris on post-infectious cough in mice

Zhao, Yun‐Li; Yang, Zifeng; Shang, Jian Ku; Huang, Wanyi; Wang, Bei; Wei, Xin; Khan, Afsar; Yuan, Zhi‐Wei; Liu, Yaping; Wang, Yifen; Wang, Xinhua; Luo, Xiao‐Dong

doi:10.1016/j.jep.2018.02.040

Cited by 35 publications

(20 citation statements)

References 72 publications

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“…Moreover, the extract and alkaloids of A. scholaris leaves have antitussive, anti-asthmatic, expectorant [29], analgesic, anti-inflammatory [30], anti-airway inflammation [31], and anti-allergic effects [32] and provide protection against postinfectious cough in vivo [33]. The alkaloids also trigger the activation of β 2 adrenergic receptors [34] and inhibit nuclear factor-κB bioactivities in vitro [35].…”

Section: Introductionmentioning

confidence: 99%

Acute and Chronic Toxicity of Indole Alkaloids from Leaves of Alstonia scholaris (L.) R. Br. in Mice and Rats

Zhao

Su²,

Shang

et al. 2020

Nat. Prod. Bioprospect.

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Alstonia scholaris (L.) R. Br. (Apocynaceae) is an evergreen tree that has been used to treat lung diseases. In this study, the toxicity profile of indole alkaloids from leaves of A. scholaris was investigated. In acute toxicity tests, mice were administered total alkaloids (TA) and five indole alkaloids. In a chronic toxicity test, rats were continuously administered TA (50, 100, and 300 mg/kg bw) for 13 weeks, followed by a 4-week recovery. A single administration of TA affected the behavior of mice, and at 12.8 g/kg bw, prone position, shortness of breath, wheezing, and convulsion were observed. The half-lethal dose (LD 50) in mice was 5.48 g/kg bw, almost 2740 times the clinical dose in humans. Among the five indole alkaloids, the maximum tolerance dose in mice ranged from 0.75 to 4 g/kg bw. The TA-treated rats did not die and showed no adverse effects or dose-dependent changes in weight or food and water consumption, despite fluctuations in hematological and biochemical parameters compared with historical data. Furthermore, both gross and histopathological observations revealed no abnormalities in any organ. With daily oral administration to rats, the non-observed-adverse-effect-level of TA was 100 mg/ kg bw. The results indicate that TA is safe for clinical use.

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Section: Introductionmentioning

confidence: 99%

Acute and Chronic Toxicity of Indole Alkaloids from Leaves of Alstonia scholaris (L.) R. Br. in Mice and Rats

Zhao

Su²,

Shang

et al. 2020

Nat. Prod. Bioprospect.

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“…In another study, Yun-Li Zhao et al evaluated the effect of 20, 24, 30, and 75 in post-infectious cough in mice (Zhao et al 2018). The animal model was standardized by instilling lipopolysaccharide (LPS-at 80 µg/50 µL/mouse) at tracheal region followed by 30 min exposure of cigarette smoke till 30 days.…”

Section: Antiallergic and Antitussive Effectsmentioning

confidence: 99%

Pharmaceutical Application of Bio-actives from Alstonia Genus: Current Findings and Future Directions

Paul

George

Yadav

et al. 2020

Advanced Structured Materials

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Genus Alstonia is comprised of around 155 species throughout the world. Phytochemical screening of Alstonia genus has demonstrated the presence of diverse phytochemicals that include at least 800 distinct metabolites. The main classes of metabolites are alkaloids, iridoids, flavonoids, fatty acids, etc. Secondary metabolites from this genus also bring positive results when employed as anticancer, anti-spasmodic, antitussive, antiarthritic, antioxidant, etc. Alstonia boonei is listed as an antimalarial drug in African pharmacopoeia. In China, a formulation consists of A. scholaris leaves-Dengtaiye tablets are used for the treatment of cough and fever symptoms. Recently, A. scholaris leaves-derived indole alkaloids have been registered as an investigational new botanical drug (No. 2011L01436) and China Food and Drug Administration (CFDA) has approved its phase I/II clinical trials for the treatment of respiratory diseases. Pharmacokinetic and safety analysis of this botanical drug in healthy human subjects have revealed a safe profile under the dose regimen experiment. Apart from that, strictamine, an indole alkaloid isolated from A. scholaris exhibited a similar in vitro antiviral activity to that of acyclovir. The present chapter is mainly focused on the critical analysis of various secondary metabolites isolated from Alstonia (approximately 15 species). Apart from that, pharmacological, toxicological and intellectual property rights studies have also been included.

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“…The total alkaloids extract (TA) was obtained from the leaves of A. scholaris by extraction with ethyl acetate, and indole alkaloid content was more than 50%. Various laboratory-identified biological activities have been reported by our research group, including antitussive, anti-asthmatic, expectorant [ 11 ], analgesic, anti-inflammatory [ 12 ], anti-airway inflammation [ 13 ], anti-allergic asthma [ 14 ], anti-postinfectious cough [ 15 ], alleviating emphysema [ 16 ] and pulmonary fibrosis [ 17 ]. The anti-inflammatory effects were achieved by triggering the β 2 adrenergic receptor [ 18 ] and inhibiting the nuclear factor-κB expression [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-microbial Effects In Vitro and In Vivo of Alstonia scholaris

Zhao

Gou

Shang

et al. 2021

Nat. Prod. Bioprospect.

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Alstonia scholaris could be used as a traditional medicinal plant in China for the treatment of acute respiratory, which might be caused by respiratory tract infections. The investigation tested the anti-infective effects of total alkaloids extract (TA) from leaves of A. scholaris, and as a result, TA inhibited herpes simplex virus type 1 (HSV-1), respiratory syncytial virus (RSV) and influenza A virus (H1N1) in vitro respectively. In addition, the survival days of mice were prolonged, and the lung weights and mortality of mice were decreased significantly, after oral administrated TA in H1N1 and beta-hemolytic streptococcus infectious models in vivo respectively. The finding supported partly the traditional usage of A. scholaris in the treatment of respiratory infections. Graphic Abstract

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Effects of indole alkaloids from leaf of Alstonia scholaris on post-infectious cough in mice

Cited by 35 publications

References 72 publications

Acute and Chronic Toxicity of Indole Alkaloids from Leaves of Alstonia scholaris (L.) R. Br. in Mice and Rats

Acute and Chronic Toxicity of Indole Alkaloids from Leaves of Alstonia scholaris (L.) R. Br. in Mice and Rats

Pharmaceutical Application of Bio-actives from Alstonia Genus: Current Findings and Future Directions

Anti-microbial Effects In Vitro and In Vivo of Alstonia scholaris

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