Effects of increased plasma viscosity and red blood cell aggregation on blood viscosity in vivo

Gustafsson, Linnéa; Appelgren, L.; Myrvold, H. E.

doi:10.1152/ajpheart.1981.241.4.h513

Cited by 26 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relation of whole blood viscosity to the microcirculation is under debate and should be discussed with caution [26], as it was demonstrated that the viscosity of whole blood tends towards the values of plasma viscosity in small capillary tubes [27,28]. Therefore, it is possible that plasma viscosity plays a major role in microcirculatory blood supply.…”

Section: Discussionmentioning

confidence: 99%

Changes of Hemorheological and Biochemical Parameters after Plasma Perfusion Using a Tryptophan-Polyvinyl Alcohol Adsorber Leading to Clinical Improvement in Patients Suffering from Maculopathy

Widder¹,

Brunner²,

Engels³

et al. 1998

Blood Purif

View full text Add to dashboard Cite

Background: Selective adsorption is an extracorporeal treatment able to reduce high-molecular-weight proteins and lipids. We evaluated its efficacy in lowering hemorheological parameters to achieve a better microcirculation of the retina. Patients and Methods: Ten patients suffering from maculopathies of various origin underwent a selective plasma adsorption procedure using the TR-350. Plasma and whole blood viscosity, erythrocyte aggregation and proteins and lipids were determined before and 24 h after therapy. Results: Selective adsorption therapy reduced the high-molecular-weight proteins and lipids. Plasma viscosity, standardized whole blood viscosity and erythrocyte aggregation were significantly lowered to 87, 88 and 65%, respectively, of their values prior to treatment. An improvement of visual acuity was achieved in 6/10 patients. Minor side effects were noted in 2/10 patients. Conclusions: Selective adsorption using the TR-350 adsorber is a safe technique, showing a high impact on blood rheology. The changes of hemorheological parameters led to clinical improvement in 6/10 patients suffering from retinal disorders.

show abstract

Section: Discussionmentioning

confidence: 99%

Changes of Hemorheological and Biochemical Parameters after Plasma Perfusion Using a Tryptophan-Polyvinyl Alcohol Adsorber Leading to Clinical Improvement in Patients Suffering from Maculopathy

Widder¹,

Brunner²,

Engels³

et al. 1998

Blood Purif

View full text Add to dashboard Cite

show abstract

“…An insidious problem with the use of dextrans is that they may interact with vessel walls and change the functional properties of the endothelium or cause changes in blood viscosity (Gustafsson et al, 1981;Pearson and Lipowsky, 2000;Bendayan, 2002;Gonzalez-Castillo et al, 2003;Tsai et al, 2003). Specifically, dextrans can bind irreversibly to the coronary vessel lumen in a mass-dependent manner and modulate coronary blood flow (GonzalezCastillo et al, 2003).…”

Section: Experimental Issues Related To Use Of Dextrans As Molecular mentioning

confidence: 99%

In vivo delivery of fluoresceinated dextrans to the murine growth plate: Imaging of three vascular routes by multiphoton microscopy

et al. 2005

View full text Add to dashboard Cite

Bone elongation by endochondral ossification occurs through the differentiation cascade of chondrocytes of cartilaginous growth plates. Molecules from the systemic vasculature reach the growth plate from three different directions: epiphyseal, metaphyseal, and a ring vessel and plexus associated with the perichondrium. This study is an analysis of the real-time dynamics of entrance of fluoresceinated tracers of different molecular weights into the growth plate from the systemic vasculature and tests the hypothesis that molecular weight is a key variable in the determination of both the directionality and the extent of tracer movement into the growth plate. Multiphoton microscopy was used for direct in vivo imaging of the murine proximal tibial growth plate in anesthetized 4-to 5-week-old transgenic mice with green fluorescent protein linked to the collagen II promoter. Mice were given an intracardiac injection of either fluorescein (332.3 Da) or fluoresceinated dextrans of 3, 10, 40, 70 kDa, singly or sequentially. For each tracer, directionality and rate of arrival, together with extent of movement within the growth plate, were imaged in real time. For small molecules (up to 10 kDa), vascular access from all three directions was observed and entrance was equally permissive from the metaphyseal and the epiphyseal sides. Within our detection limit (a few percent of vascular concentration), 40 kDa and larger dextrans did not enter. These results have implications both for understanding systemic and paracrine regulation of growth plate chondrocytic differentiation, as well as variables associated with effective drug delivery to growth plate chondrocytes. Key words: growth plate; vasculature; multiphoton microscopy; endochondral ossificationBone elongation in children occurs by endochondral ossification in cartilaginous growth plates at the ends of long bones. Chondrocytic differentiation in the growth plate begins with clonal expansion of stem cells, continues during cellular proliferation, and ends with cellular enlargement, followed by death and replacement by bone. The kinetics of chondrocytic activity in each stage and of the regulatory transitions between them determine the rate of bone elongation achieved. As in any cellular growth system, nutrients are required to sustain the process, and multiple endocrine and paracrine inputs regulate at each stage. For growth plate cartilage, the role of the extracellular matrix also must be considered, not only as a substantive contributor to elongation per se, but through its potential role as a communication link among chondrocytes of the growth plate, and between growth plate chondrocytes and cells of surrounding tissues such as the peri-

show abstract

“…This phenomenon was known since long ago, but the relationship between the RBCs aggregation (as cause) and the increase of resistance disturbing the flow (as result) in microvessels was difficult to prove convincingly because some inevitable factors, such as the pressure gradient and/or the microvascular diameter changes, could always be involved in the living microvascular networks. The effect of the RBCs aggregation on blood flow was largely examined by infusing macromolecules, preferably dextran with high molecular weight, into isolated organs [60][61][62] and in patients with serum hyperviscosity syndrome [63,64]. Under these conditions it was difficult to prove whether the increased flow resistance was due to the RBCs aggregation itself or to the increased plasma viscosity.…”

Section: Rbcs Aggregation As a Factor Inevitably Disturbing Blood Flomentioning

confidence: 99%

Blood Flow Structure Related to Red Cell Flow: Determinant of Blood Fluidity in Narrow Microvessels

Mchedlishvili

Maeda²

2001

Jpn.J.Physiol

112

104

View full text Add to dashboard Cite

Blood advancing in living capillaries (5 to 10 m in diameter) and in the adjoining arterioles and venules (10 to 25 m in diameter) itself represents a specific substance hardly comparable with fluids in a usual understanding of this term. Its greatest part comprises the deformed red blood cells (RBCs) whose size is similar to luminal diameters of the microvessels. The RBCs (comprising great majority of the forming elements in microvessels) are disposed in the normal flow not chaotically, but in a certain order that was identified therefore as "blood flow structure (or structuring) in microvessels" [1][2][3].The specific regime of the RBCs flow in blood vessels changes from larger to smaller luminal diameters. Thus in vessels larger than 0.2 mm, the blood flow can be treated as a homogenous suspension with little error. By contrast, in arterioles or venules smaller than 25 m, and especially in capillaries (whose diameters are smaller than 8 m in humans), the RBCs are not uniformly dispersed, especially because of the presence of a parietal plasma layer containing no cells; thus the blood flow is specifically structured (Fig. 1). Disorders of this kind inevitably lead to the disturbance of normal blood rheological properties in the microvessels.Japanese Journal of Physiology Vol. 51, No. 1, 2001 19Japanese Journal of Physiology, 51, 19-30, 2001 Key words: RBC axial flow, parietal plasma layer, RBC aggregation, RBC deformation, plasma viscosity. Abstract:The review article deals with phenomena of the blood flow structure (structuring) in narrow microvessels-capillaries and the adjacent arterioles and venules. It is particularly focused on the flow behavior of red blood cells (RBCs), namely, on their specific arrangements of mutual interaction while forming definite patterns of self-organized microvascular flow. The principal features of the blood flow structure in microvessels, including capillaries, include axial RBC flow and parietal plasma layer, velocity profile in larger microvessels, plug (or bolus) flow in narrow capillaries, and deformation and specific behavior of the RBCs in the flow. The actual blood flow structuring in microvessels seems to be a most significant factor in the development of pathological conditions, including arterial hypertension, brain and cardiac infarctions, inflammation, and many others. The blood flow structuring might become a basic concept in determining the blood rheological properties and disorders in the narrow microvessels. No solid theoretical (biorheological) basis of the blood flow structuring in microvessel has been found, but in the future it might become a foundation for a better understanding of the mechanisms of these properties under normal and pathological conditions in the narrowest microvessels 5 to 25 m large. It is also a topic for further biorheological research directed to find the background of actual physiopathological phenomena in the microcirculation.

show abstract

Effects of increased plasma viscosity and red blood cell aggregation on blood viscosity in vivo

Cited by 26 publications

References 0 publications

Changes of Hemorheological and Biochemical Parameters after Plasma Perfusion Using a Tryptophan-Polyvinyl Alcohol Adsorber Leading to Clinical Improvement in Patients Suffering from Maculopathy

Changes of Hemorheological and Biochemical Parameters after Plasma Perfusion Using a Tryptophan-Polyvinyl Alcohol Adsorber Leading to Clinical Improvement in Patients Suffering from Maculopathy

In vivo delivery of fluoresceinated dextrans to the murine growth plate: Imaging of three vascular routes by multiphoton microscopy

Blood Flow Structure Related to Red Cell Flow: Determinant of Blood Fluidity in Narrow Microvessels

Contact Info

Product

Resources

About