Effects of in vivo exposure to tritium: a multi-biomarker approach using the fathead minnow, Pimephales promelas

Gagnaire, Béatrice; Gosselin, Isabelle; Festarini, Amy; Walsh, Stephanie; Cavalié, Isabelle; Adam-Guillermin, Christelle; Della-Vedova, Claire; Farrow, F.; Kim, Sang Bog; Shkarupin, Alexi; Chen, Hui Qun; Beaton, Danielle; Stuart, Marilyne

doi:10.1007/s11356-018-3781-5

Cited by 8 publications

(7 citation statements)

References 47 publications

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“…Increase of ROS production was also shown for the different stressors, but not for all stages or all dose rates studied. These results confirm the known data on the effects of ionizing radiation in terms of oxidizing stress and genotoxicity , Gagnaire et al 2017, Gagnaire et al 2020b, Hu et al 2016.…”

Section: Comparison Of Results For Hto/tritiated Thymidine/gamma Radiation Exposuresupporting

confidence: 91%

“…The nervous system did not seem to be a target of HTO in zebrafish larvae, contrary to gamma irradiation. However, AChE activity was modified in adult fathead minnows exposed to HTO after depuration (Gagnaire et al 2020b). Moerover, genes involved in muscle contraction were modified by HTO (Arcanjo et al 2018), and swimming behavior of larvae was altered but only for one dose rate (Arcanjo et al 2020).…”

Section: Comparison Of Results For Hto/tritiated Thymidine/gamma Radiation Exposurementioning

confidence: 99%

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Effects of gamma ionizing radiation exposure on Danio rerio embryo-larval stages - comparison with tritium exposure

Gagnaire

Arcanjo

Cavalié

et al. 2021

Journal of Hazardous Materials

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The objective was to investigate the effects of ionizing radiation induced in zebrafish early life stages by coupling responses obtained at the molecular (genotoxicity, ROS production, gene expression) and phenotypic (tissue alteration, embryo-larval development) levels. Here we present results obtained after exposure of 3 hpf larvae to 10 days of gamma irradiation at 3.3x10 1 , 1.3x10 2 and 1.2x10 3 µGy/h, close to and higher than the benchmark for protection of ecosystems towards ionizing radiations of 10 1 µGy/h. Dose rates used in these studies were chosen to be in the 'derived consideration reference level' (DCRL) for gamma irradiation where deleterious effects can appear in freshwater fish. Also, these dose rates were similar to the ones already tested on tritium (beta ionizing radiation) in our previous work, in order to compare both types of ionizing radiation. Results showed that gamma irradiation did not induce any effect on survival and hatching. No effect was observed on DNA damages, but ROS production was increased. Muscle damages were observed for all tested dose rates, similarly to previous results obtained with tritium (beta ionizing radiation) at similar dose rates. Some molecular responses therefore appeared to be relevant for the study of gamma ionizing radiation effects in zebrafish.

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Section: Comparison Of Results For Hto/tritiated Thymidine/gamma Radiation Exposuresupporting

confidence: 91%

Section: Comparison Of Results For Hto/tritiated Thymidine/gamma Radiation Exposurementioning

confidence: 99%

Effects of gamma ionizing radiation exposure on Danio rerio embryo-larval stages - comparison with tritium exposure

Gagnaire

Arcanjo

Cavalié

et al. 2021

Journal of Hazardous Materials

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“…Exchanges of HTO between water and organisms are rapid: maximal dose rates were obtained for 24‐h eggs. Several studies showed that tritium accumulated in adult organisms equilibrates with HTO concentration of the water and that the fraction of tritium bound to organic matter equilibrates with the concentration of HTO internalized (Galeriu and Melintescu ; Kim and Korolevych ; Melintescu et al ; Kim et al , ; Gagnaire et al , ). A companion paper study presented in detail the discussion about internalization results obtained after tritium contamination for 2 stages (24‐h eggs and 4‐dpf larvae) and 2 HTO concentrations (10 5 and 10 6 Bq/mL; Arcanjo et al ).…”

Section: Discussionmentioning

confidence: 99%

“…Exchanges of HTO between water and organisms are rapid: maximal dose rates were obtained for 24-h eggs. Several studies showed that tritium accumulated in adult organisms 7: Relative expression of genes (normalized to reference gene ef1) compared to control in larvae contaminated during 10 d at 10 4 , 10 5 , and 10 6 Bq/mL of tritiated water (n = 9) a 4 dpf 7 dpf 10 dpf 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL ache equilibrates with HTO concentration of the water and that the fraction of tritium bound to organic matter equilibrates with the concentration of HTO internalized (Galeriu and Melintescu 2011;Kim and Korolevych 2013;Melintescu et al 2015;Kim et al 2015Kim et al , 2019Gagnaire et al 2017Gagnaire et al , 2020. A companion paper study presented in detail the discussion about internalization results obtained after tritium contamination for 2 stages (24-h eggs and 4-dpf larvae) and 2 HTO concentrations (10 5 and 10 6 Bq/mL; Arcanjo et al 2019).…”

Section: Dose Rates Calculated For Htomentioning

confidence: 99%

Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

Gagnaire

Arcanjo

Cavalié

et al. 2020

Enviro Toxic and Chemistry

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Tritium, a radioactive isotope of hydrogen of natural and anthropogenic origin, is ubiquitously present in the environment. Effluents of nuclear centers of production are significant anthropogenic sources. With the upcoming project of thermonuclear fusion, tritium releases in the environment may increase. It is therefore important to characterize the ecological risk linked to tritium. The effects of tritiated water (HTO) were therefore studied in zebrafish larvae exposed for 10 d to different dose rates, 1.1 × 102, 4.1 × 102, and 3.8 × 103 µGy/h for larvae corresponding, respectively, to a water contamination of 104, 105, and 106 Bq/mL of HTO. Those dose rates were higher than 10 μGy/h, which is the threshold recommended to start monitoring ecosystems where radiological contaminants are present. Mortality, embryo‐larval development, immune toxicity, genotoxicity, neurotoxicity, and alterations of tissues were investigated. The results showed that HTO exposure induced DNA damage and reactive oxygen species production and modulated the expression of genes involved in detoxification processes. Moreover, modifications of the muscular tissues (degradation of myofibrils at 4 d post fertilization and disorganization of mitochondria at later stages) were observed. The results differed with HTO dose rates and with developmental stages. These results will drive future research for the development of new HTO‐sensitive biomarkers and will allow us to progress in the characterization of the modes of action of tritium in fish. Environ Toxicol Chem 2020;39:648–658. © 2019 SETAC

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“…The goal of this study was to adapt, optimize and validate the NBT assay (as described by [4] and [3] ) for use with kidney cells harvested from the fathead minnow ( Pimephales promelas ). To our knowledge, this assay has not yet been utilized to assess respiratory burst in this species, a common model for assessing general toxicity and emerging model in the field of immunotoxicity [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] . This study explored the following experimental parameters to maximize the performance of the respiratory burst assay: 1) PMA concentration, 2) incubation time, 3) erythrocyte lysis, and 4) presence of superoxide dismutase (SOD).…”

Section: Methods Detailsmentioning

confidence: 99%

A practical guide for assessing respiratory burst and phagocytic cell activity in the fathead minnow, an emerging model for immunotoxicity

2020

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Measures of respiratory burst and phagocytic cell activity are frequently utilized to assess cellular immune function in teleosts. Respiratory burst predominately occurs in neutrophils and causes the release of reactive oxygen species to kill pathogens. Phagocytosis is the process by which pathogens are engulfed and destroyed by various immune cells. Though a variety of approaches have been utilized to measure respiratory burst and phagocytic cell activity, assays that rely only on common laboratory equipment (e.g., plate reader) may offer advantages over those that rely on more specialized equipment (e.g., flow cytometer). The goal of the current study was to optimize and validate the use of a colorimetric plate-based respiratory burst and fluorometric plate-based phagocytic cell activity assays for use with kidney cells from the fathead minnow ( Pimephales promelas ), an emerging immunotoxicity model. In addition, a protocol for the dissection of kidney tissue followed by the extraction of kidney cells, as well as recommendations and resources for future experiments utilizing each of these assays, are provided. All methods are optimized for use with the fathead minnow or similar teleost species. Respiratory burst and phagocytic cell activity are measured using a standard plate reader.

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Effects of in vivo exposure to tritium: a multi-biomarker approach using the fathead minnow, Pimephales promelas

Cited by 8 publications

References 47 publications

Effects of gamma ionizing radiation exposure on Danio rerio embryo-larval stages - comparison with tritium exposure

Effects of gamma ionizing radiation exposure on Danio rerio embryo-larval stages - comparison with tritium exposure

Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

A practical guide for assessing respiratory burst and phagocytic cell activity in the fathead minnow, an emerging model for immunotoxicity

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