Effects of in vitro exposure to power frequency magnetic fields on UV‐induced DNA damage of rat lymphocytes

Zmyślony, Marek; Palus, Jadwiga; Dziubałtowska, Elżbieta; Politański, Piotr; Mamrot, Paweł; Rajkowska, Elżbieta; M, Kameduła

doi:10.1002/bem.20050

Cited by 11 publications

(6 citation statements)

References 16 publications

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“…Also, it has been reported that ELF MF could potentiate the DNA damage induced by H 2 O 2 (74) or influence the radical pairs generation during the oxidative or enzymatic processes of DNA repair (75). Herein reported results show that a 42-h exposure to MF potentiates the ROL-induced stimulation of DNA replication and cell growth in NB69 cells.…”

Section: Discussionmentioning

confidence: 53%

Influence of a 50 Hz magnetic field and of all-trans‑retinol on the proliferation of human cancer cell lines

Trillo

2012

Int J Oncol

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Abstract. In vitro exposure to power frequency magnetic fields (MF) has been reported to influence cell proliferation and differentiation. However, the nature of the response of different human cancer cell types to these fields has not been sufficiently characterized. The present work investigates the response of two proliferating human cell lines of neuroblastoma (NB69) and hepatocarcinoma (HepG2) to a 42 h, intermittent treatment with a weak, 100 µT, 50 Hz MF, alone or in combination with 0.5 µM all-trans-retinol (ROL), a retinoid currently applied in oncostatic therapies. In each experimental replicate the cell samples were submitted to one of the following treatment combinations: MF+/ROL+, MF+/ ROL-, MF-/ROL+ or MF-/ROL-. The proliferative response was determined by cell counting (Trypan blue exclusion), BrdU incorporation and by spectrophotometric analysis of total protein and DNA content. The results show that when administered separately, the two treatments, MF and ROL, significantly enhanced cell proliferation in both cell lines. In NB69 simultaneous administration of MF and ROL induced an additive effect on cell proliferation, associated to increased DNA content. By contrast, in HepG2 the ROL-induced cell proliferation and increased protein content were partially blocked by simultaneous exposure to MF. Taken together, these data show that both agents, a weak MF and ROL at a low concentration, induce proliferative responses in the two assayed human cell lines. However, significant differences were observed between the responses of the two cellular species to the combined treatment with ROL and MF, indicating that the mechanisms underlying the cellular response to each of the two agents can mutually interact in a manner that is cell type-specific.

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Section: Discussionmentioning

confidence: 53%

Influence of a 50 Hz magnetic field and of all-trans‑retinol on the proliferation of human cancer cell lines

Trillo

2012

Int J Oncol

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“…Nindl et al [] studied the effect of simultaneous exposure to a magnetic field at 1 mT and 100 Hz and UV‐B followed by magnetic field exposure alone against DNA synthesis and repair in Jurkat cell, and reported that the magnetic field enhanced the lymphocyte cell killing effect. Zmyślony et al [] investigated the potential interaction between the magnetic field at 40 µT and 50 Hz and UV‐A in rat lymphocytes, and reported that the magnetic field exposure significantly enhanced the damage induced by UV‐A irradiation. The exposure to a magnetic field and UV‐A was started simultaneously, but UV‐A irradiation was ended after 5 min and the magnetic field was continued for 60 min.…”

Section: Discussionmentioning

confidence: 99%

ELF magnetic fields do not affect cell survival and DNA damage induced by ultraviolet B

Mizuno

Narita

Yamada

et al. 2013

Bioelectromagnetics

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We investigated whether extremely low frequency (ELF) magnetic field exposure has modification effects on cell survival after ultraviolet B (UV-B) irradiation and on repair process of DNA damage induced by UV-B irradiation in WI38VA13 subcloned 2RA and XP2OS(SV) cells. The ELF magnetic field exposure was conducted using a Helmholtz coil-based system that was designed to generate a sinusoidal magnetic field at 5 mT and 60 Hz. Cell survival was assessed by WST assay after UV-B irradiation at 20-80 J/m(2) , ELF magnetic field exposure for 24 h, followed by incubation for 48 h. DNA damage was assessed by quantification of cyclobutane pyrimidine dimer formation and 6-4 photoproduct formation using ELISA after UV-B irradiation at 20-80 J/m(2) followed by ELF magnetic field exposure for 24 h. No significant changes were observed in cell survival between ELF magnetic field and sham exposures. Similarly, DNA damage induced by UV-B irradiation did not change significantly following ELF magnetic field exposure. Our results suggest that ELF magnetic field exposure at 5 mT does not have modification effect on cell survival after UV-B irradiation and on repair process of DNA damage induced by UV-B irradiation.

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“…Within the range of household frequency that we tested (50/60 Hz), some studies find no evidence for cell stress in bacteria [Nakasono and Saiki, 2000] or human cells [Coulton et al, 2004]. Others provide evidence that MF causes cellular damage [Zmyslony et al, 2004], and/or expression of stress-responsive proteins [Lin et al, 1997;Cairo et al, 1998;Goodman and Blank, 2002;Robison et al, 2002;Tokalov and Gutzeit, 2004] which serve as biological markers of cell stress. Cells respond to harsh physiological conditions by inducing the expression of protective stress proteins like heat-shock proteins.…”

Section: Mf-induced Secondary Cross-stress Protection In the Viabilitmentioning

confidence: 96%

14.6 mT ELF magnetic field exposure yields no DNA breaks in model systemSalmonella, but provides evidence of heat stress protection

Williams

Ingebretsen

Dawson

2006

Bioelectromagnetics

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In this study, we demonstrate that common extremely low frequency magnetic field (MF) exposure does not cause DNA breaks in this Salmonella test system. The data does, however, provide evidence that MF exposure induces protection from heat stress. Bacterial cultures were exposed to MF (14.6 mT 60 Hz field, cycled 5 min on, 10 min off for 4 h) and a temperature-matched control. Double- and single-stranded DNA breaks were assayed using a recombination event counter. After MF or control exposure they were grown on indicator plates from which recombination events can be quantified and the frequency of DNA strand breaks deduced. The effect of MF was also monitored using a recombination-deficient mutant (recA). The results showed no significant increase in recombination events and strand breaks due to MF. Evidence of heat stress protection was determined using a cell viability assay that compared the survival rates of MF exposed and control cells after the administration of a 10 min 53 degrees C heat stress. The control cells exhibited nine times more cell mortality than the MF exposed cells. This Salmonella system provides many mutants and genetic tools for further investigation of this phenomenon.

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Effects of in vitro exposure to power frequency magnetic fields on UV‐induced DNA damage of rat lymphocytes

Cited by 11 publications

References 16 publications

Influence of a 50 Hz magnetic field and of all-trans‑retinol on the proliferation of human cancer cell lines

Influence of a 50 Hz magnetic field and of all-trans‑retinol on the proliferation of human cancer cell lines

ELF magnetic fields do not affect cell survival and DNA damage induced by ultraviolet B

14.6 mT ELF magnetic field exposure yields no DNA breaks in model systemSalmonella, but provides evidence of heat stress protection

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