Effects of In Utero Exposure to Di-n-Butyl Phthalate on Testicular Development in Rat

Ma, Tan; Yin, Xiaoqin; Han, Ruitong; Ding, Jie; Zhang, Huan; Han, Xiaodong; Li, Dongmei

doi:10.3390/ijerph14101284

Cited by 38 publications

(22 citation statements)

References 50 publications

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“…As an environmental endocrine disruptor, DBP has certain male reproductive toxicity, can cause male rodents testicular atrophy, weight loss, decreased testicular activity of the enzyme, seminiferous tubules atrophy, spermatogenic cell loss, genital malformations, etc. [17]. In this vivo study of testicular pathology showed that DBP lead to testicular tissue structure damage, manifested as spermatogenic tubule disorders, Sertoli cell vacuolization, germ cell shedding, and so on.…”

Section: Discussionmentioning

confidence: 99%

Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)

et al. 2019

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Background Di- N -butyl-phthalate (DBP) is an endocrine disrupting substance. We investigated the adverse effect of DBP on testis of male rat and reveal its potential mechanism of MAPK signaling pathway involved this effect in vivo and in vitro. Gonadal hormone, sperm quality, morphological change and the activation status of JNK, ERK1/2 and p38 was determined in vivo. Primary Sertoli cell was established and cultivated with JNK, ERK1/2 inhibitors, then determine the cell viability, apoptosis and the expression of p-JNK, p-ERK1/2. Data in this study were presented as mean ± SD and determined by one-way analysis of variance (ANOVA) followed by Bonferroni’s test. Difference was considered statistically significant at P < 0.05. Results In vivo experiment, DBP impaired the normal structure of testicular tissue, reduced testosterone levels in blood serum, decreased sperm count and increased sperm abnormality, p-ERK1/2 and p-JNK in rat testicular tissue increased in a dose-dependent manner. In vitro studies, DBP could decrease the viability of Sertoli cells and increase p-ERK1/2 and p-JNK. Cell apoptosis in SP600125 + DBP group was significantly lower than in DBP group (P < 0.05). p-JNK was not significantly decreased in SP600125 + DBP group, while p-ERK1/2 was significantly decreased in U0126 + DBP group. Conclusions These results suggest that DBP can lead to testicular damage and the activation of ERK1/2 and JNK pathways, the JNK signaling pathway may be primarily associated with its effect.

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Section: Discussionmentioning

confidence: 99%

Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)

et al. 2019

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“…In recent decades, male reproductive problems, such as spermatogenesis disorders, infertility, and premature abortion, have become more prevalent worldwide [1]. It is worth noting that infertility affects an estimated 15% of couples globally, and males were believed to contribute to 50% of cases overall [2].…”

Section: Introductionmentioning

confidence: 99%

Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats

Guo

Lin

Xue

et al. 2019

IJERPH

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Under some occupational conditions, workers are inevitably exposed to high-intensity radiofrequency (RF) fields. In this study, we investigated the effects of one-month exposure to a 220 MHz pulsed modulated RF field at the power density of 50 W/m2 on the sperm quality in male adult rats. The sperm quality was evaluated by measuring the number, abnormality and survival rate of sperm cells. The morphology of testis was examined by hematoxylin–eosin (HE) staining. The levels of secreting factors by Sertoli cells (SCs) and Leydig cells (LCs) were determined by enzyme linked immunosorbent assay (ELISA). The level of cleaved caspase 3 in the testis was detected by immunofluorescence staining. Finally, the expression levels of the apoptosis-related protein (caspase 3, BAX and BCL2) in the testis were assessed by Western blotting. Compared with the sham group, the sperm quality in the RF group decreased significantly. The levels of secreting factors of SCs and the morphology of the testis showed an obvious change after RF exposure. The level of the secreting factor of LCs decreased significantly after RF exposure. The levels of cleaved caspase 3, caspase 3, and the BAX/BCL2 ratio in the testis increased markedly after RF exposure. These data collectively suggested that under the present experimental conditions, 220 MHz pulsed modulated RF exposure could impair sperm quality in rats, and the disruption of the secreting function of LCs and increased apoptosis of testis cells induced by the RF field might be accounted for by this damaging effect.

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“…Some formulations of 5-ASA drugs include dibutyl phthalate (DBP) in the enteric coating, which had been associated with skeletal formations and urogenital defects in male offspring of exposed female rates. 35,36 In a report of six cases, urinary concentrations of phthalate metabolites in patients treated with DBP-containing 5-ASA formulations were 50 times higher than those not treated with these formulations. 37 The effects seen in animal models have not been seen in humans.…”

Section: Pharmacologic Therapiesmentioning

confidence: 98%

Insights into the treatment of inflammatory bowel disease in pregnancy

Shannahan

Erlich

Peppercorn

2019

Therap Adv Gastroenterol

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Patients diagnosed with inflammatory bowel disease (IBD) are most commonly diagnosed in late adolescence or early adulthood, with half of patients being diagnosed before age 32, thus impacting peak years of reproduction and family planning. While controlled IBD has no negative effects on the ability to conceive, there is overall a trend towards voluntary childlessness due to patients’ concerns for adverse fetal outcomes from underlying IBD and from adverse medication effects. Active disease at the time of conception is associated with worsening disease activity during pregnancy and carries a higher risk of poor fetal outcomes. It is therefore important to maintain remission during pregnancy, which is often achieved with pharmacologic therapy. The goal of this paper is to provide a comprehensive review of the current literature and safety data for pharmacologic treatment of IBD in pregnancy, in breastfeeding women, and in men planning to have children.

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Effects of In Utero Exposure to Di-n-Butyl Phthalate on Testicular Development in Rat

Cited by 38 publications

References 50 publications

Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)

Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)

Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats

Insights into the treatment of inflammatory bowel disease in pregnancy

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