Effects of In Ovo Supplementation with Nanonutrition (L-Arginine Conjugated with Ag NPs) on Muscle Growth, Immune Response and Heat Shock Proteins at Different Chicken Embryonic Development Stages

Subramaniyan, Sivakumar Allur; Kang, Darae; Siddiqui, Sharif Hasan; Park, Jinryong; Tian, Weishun; Park, Byung-Yong; Shim, Kwan Seob

doi:10.3390/ani10040564

Cited by 5 publications

(4 citation statements)

References 32 publications

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“…Taurine, supplemented in ovo to chicken embryos, improved the structure of breast muscle by increasing the expression of PCNA protein [ 6 ]. L-Arginina conjugated with nanoparticles of Ag activates myogenin and MyoD expression, which is pointed to improve muscle growth [ 7 ]. Due to the fact that embryonic development takes more than 1/3 of the life of broiler chickens and is a key period for muscle development, the method of in ovo feeding is very promising [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Muscle Extract and Graphene Oxide on Muscle Structure of Chicken Embryos

Bałaban

Zielińska

Wierzbicki

et al. 2021

Animals

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The effects of CEME and it complex with GO injected in ovo on the growth and development of chicken embryo hindlimb muscle were investigated. First, the preliminary in vitro study on primary muscle precursor cell culture obtained from a nine-day-old chicken embryo was performed to assess toxicity (MTT assay) of CEME, GO (100 ppm) and it complex with different concentrations (1, 2, 5, and 10 wt.%). The effect on cell proliferation was investigated by BrdU assay. CEME at concentrations 1–5% increased cell proliferation, but not the complex with GO. In vitro cytotoxicity was highest in 10% and GO groups. Next, the main experiment with chicken embryos was performed with CEME, GO and it complex injected in ovo on day one of embryogenesis. On day 20 of embryogenesis survival, morphological development, histological structure of the muscle, and biochemical parameters of blood serum of the embryos were measured. No negative effect on mortality, body weight, or biochemistry of blood after use of CEME or GO-CEME complexes was observed. Interestingly, the slight toxicity of GO, observed in in vitro studies, was not observed in vivo. The use of CEME at the levels of 2% and 5% improved the structure of the lower limb muscle by increasing the number of cells, and the administration of 2% CEME increased the number of nuclei visible in the stained cross-section of the muscle. The complex GO-CEME did not further improve the muscle structure. The results indicate that CEME can be applied as an in ovo enhancer of muscle development in broilers.

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Section: Introductionmentioning

confidence: 99%

Effect of Muscle Extract and Graphene Oxide on Muscle Structure of Chicken Embryos

Bałaban

Zielińska

Wierzbicki

et al. 2021

Animals

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“…It was documented that the expression of myogenin and MyoD was significantly upregulated by IOF of l-arginine (100 μg) on day 14 of incubation (Subramaniyan et al 2019). Likewise, Subramaniyan et al (2020) confirmed that IOF of l-arginine conjugated with silver nanoparticles (100 μg with 1 000 µg, respectively) on day 14 of incubation upregulated the protein expression of muscle development markers (myogenin and myoD). Also, Gonzales et al (2022) detected that IOF of 0.150 mg folic acid increased the expression of the Pax7 and Myf genes involved in satellite cell proliferation in the pectoralis muscle of hatched chicks.…”

Section: Impact Of In Ovo Feeding Of Nutraceuticals On Growth Perform...mentioning

confidence: 59%

“…Similarly, Gonzales et al (2022) revealed that IOF with a combination of sulphur amino acids (5.90 mg of l-methionine and 3.40 mg of l-cysteine) plus 0.150 mg of folic acid increased the relative weights of thymus and spleen at 21 days of age. Moreover, it was demonstrated that IOF of 100 μg l-arginine (Subramaniyan et al 2019) or 100 μg l-arginine conjugated with 1 000 µg silver nanoparticles (Subramaniyan et al 2020) on day 14 of incubation increased the serum concentration of IgM in newly hatched chicks. In the same line, Kermanshahi et al (2017) stated that IOF of 0.5 mg threonine on day 11 of embryogenesis upregulated IgA mRNA in the new-born chicks of Japanese quail.…”

Section: Impact Of In Ovo Feeding Of Nutraceuticals On Immune Responsementioning

confidence: 99%

In‌ ovo feeding of nutraceuticals and its role in adjusting the gastrointestinal tract, antioxidative properties, immunological response, and performance in poultry: An updated review

Ebeid¹,

Ketta²,

Al-Homidan³

et al. 2023

CZECH J ANIM SCI

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Nutraceuticals are food ingredients that provide extra health advantages that expand above their nutritional value. Nutraceuticals comprise amino acids, antioxidants, minerals, vitamins, probiotics, prebiotics, synbiotics, enzymes, organic acids, fatty acids, medicinal plants, etc. Recently, nutraceuticals have acquired much attention in poultry industry by reason of their potential effects on establishing the normal physiological status, supporting the immune system, and disease prevention, which consequently enhances productivity. The technique of in ovo feeding (IOF) of nutraceuticals holds several promises in poultry industry. The vital benefits of IOF of nutraceuticals are enhancement of intestinal development, establishment of a balanced microbial population in the gut, support of intestinal physiology and health. Interestingly, IOF of nutraceuticals participates in improving the antioxidative properties and energizing the immune system, which thereby translated into greater performance and bigger resistance to diseases, especially in early life challenges. The current review attempts to throw more light on the fresh results associated with the profits of IOF of nutraceuticals on intestinal histomorphology, intestinal microbiota, antioxidative properties, immune responsiveness, hatchability, chick quality, and growth performance in poultry.

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“…Arginine plays a crucial role in the production of polyamine and NO, which are strong anti-stress and vasodilator factors in mammals [2]. In addition, Arg facilitates the provision of cellular signaling, intestinal recovery, and immunity factors [10,14]. Therefore, it can be hypothesized that the use of dietary Arg levels in higher doses than the recommended requirement may decrease the ZnO requirement during the stressful condition.…”

Section: Introductionmentioning

confidence: 99%

Effects of Zinc Oxide and Arginine on the Intestinal Microbiota and Immune Status of Weaned Pigs Subjected to High Ambient Temperature

Yoon

Jin

Cho

et al. 2020

Animals

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This study aimed to investigate the effect of the l-arginine (Arg) inclusion and different doses of ZnO on the growth performance, intestinal microbiota and integrity, and immune status of weaned pigs. A total of 180 pigs (28-day-old) were randomly allotted to six treatments with six replicate pens in each treatment and five pigs per pen. The dietary treatments were Con (1.1% Arg); P-Zn (1.1% Arg + 2500 mg Zn as ZnO/kg diet); ARG (1.6% Arg); ZnArg1 (500 mg of Zn as ZnO/kg diet + 1.6% Arg); ZnArg2 (1000 mg of Zn as ZnO/kg diet + 1.6% Arg); ZnArg3 (2500 mg of Zn as ZnO/kg diet + 1.6% Arg). The overall result showed that the inclusion of ZnArg3 significantly improved the average daily gain of pigs compared with the Con treatment. There was a reduction in feed intake in pigs fed the Con diet compared with pigs fed the ZnArg3 diet at phase 1 and overall. At phase 1, pigs fed the ZnArg3 diet and P-Zn diet showed a decreased population of Clostridium spp. in the ileum compared with those of the Con treatment. In addition, a lower ileal Clostridium spp. population was detected in pigs fed the ZnArg2 diet compared with pigs fed the Con diet. The pigs fed ZnArg1 and ZnArg3 diets showed a greater villus height of duodenum compared with the Con and P-Zn treatments. The pigs in the Con treatment showed increased mRNA expression of heat shock protein-27 in the liver compared with the P-Zn, ZnArg1, ZnArg2, and ZnArg3 treatments. When fed the basal diet, mRNA expressions of interleukin-6 were increased in the muscle compared with the ZnArg3 treatment. Dietary supplementation with ZnArg2 decreased the mRNA expressions of interferon-γ in the muscle compared with the Con treatment. Supplementation with P-Zn, ZnArg1, ZnArg2, and ZnArg3 decreased mRNA expressions of tumor necrosis factor-α (TNF-α) compared with the Con treatment. The mRNA gene expressions of interleukin-4 were decreased in the jejunum of pigs fed P-Zn, ARG, ZnArg1, ZnArg2, and ZnArg3 diets compared with pigs fed the Con diet. The jejunum gene expression of toll-like receptor-4 was upregulated in the Con and ARG treatments compared with the ZnArg1 and ZnArg3. The ZnArg1, ZnArg2, and ZnArg3 treatments showed lower mRNA expression of TNF-α compared with the Con treatment. In conclusion, there was no difference in growth performance, intestinal microbiota, gene expression of interleukins between ZnArg1 and ZnArg3 treatments. Therefore, the low level of ZnO (500 mg/kg) plus 1.6% dietary Arg may be recommended for pigs during the weaning stress.

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Effects of In Ovo Supplementation with Nanonutrition (L-Arginine Conjugated with Ag NPs) on Muscle Growth, Immune Response and Heat Shock Proteins at Different Chicken Embryonic Development Stages

Cited by 5 publications

References 32 publications

Effect of Muscle Extract and Graphene Oxide on Muscle Structure of Chicken Embryos

Effect of Muscle Extract and Graphene Oxide on Muscle Structure of Chicken Embryos

In‌ ovo feeding of nutraceuticals and its role in adjusting the gastrointestinal tract, antioxidative properties, immunological response, and performance in poultry: An updated review

Effects of Zinc Oxide and Arginine on the Intestinal Microbiota and Immune Status of Weaned Pigs Subjected to High Ambient Temperature

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