Effects of in ovo administration of L-ascorbic acid on broiler hatchability and its influence on the effects of pre-placement holding time on broiler quality characteristics , ,

Abstract

The current study was conducted to determine the possible effects of the in ovo administration of different dosages of L-ascorbic acid (AA) to broiler hatching eggs on hatchability and its potential for reducing the adverse effects of delayed placement.A total of 702 broiler hatching eggs was hand-injected at 17 d of incubation (DOI) with 100 μL of sterile saline (0.85%) alone or containing 0.5, 1.5, 4.5, or 13.5 mg AA. Hatchability was recorded every 5 h from 480 h to 505 hours. Results showed that AA injection did not affect embryo BW as percentage of set egg weight or yolk sac weight as percentage of embryo weight at 19.5 DOI. The hatching time of all embryos that received an AA in ovo injection was between 480 and 495 h of incubation, and significantly fewer embryos hatched before 480 h in comparison to non-injected controls. Hatchability (above 92% in all groups) was not significantly affected by injection treatment. However, fertile eggs injected with saline containing 4.5 mg AA had the highest hatchability. At 21 DOI, hatching BW as a percentage of set egg weight and yolk sac weight as a percentage of BW were numerically higher in AA injection groups. An in ovo injection of AA at a 13.5 mg/egg level resulted in a numerically higher BW as a percentage of set egg weight. The in ovo injection of AA did not reduce the adverse effects of a 48-hour posthatch pre-placement holding time on BW or on yolk sac absorption. Overall, in ovo injection of L-ascorbic acid (0.5 to 13.5 mg/egg) into fertile broiler hatching eggs at 17 DOI did not negatively affect hatchability or embryo development, and did not prevent the negative effects of a 48-hour posthatch holding time on BW and yolk sac absorption. The range of tolerance as well as the optimal dosage of in ovo-injected AA warrants future study.

Effects of in ovo feeding of N-acetyl-L-glutamate on early intestinal development and growth performance in broiler chickens

“…The results of embryonic characteristics examined at 19.5 DOI indicated that all embryos, including those injected with solution, grew normally. Similar results were observed in a previous study with L-ascorbic acid injection ( Zhang et al., 2018a ). Greater synthesis and lower degradation of proteins were observed in embryos injected with nutrition ( Ohta and Kidd, 2001 ).…”

“…The eggs had an average weight of 59.28 ± 0.07 g and were stored in an egg storage facility under commercial conditions. The eggs were warmed to room temperature (25°C) before being set ( Zhang et al., 2018a ) and incubated in a micro-computer automatic incubator (Model: BLF-J3520, Chengdu Beili Agricultural Technology Co., Ltd., Chengdu, China) at Nankou Experimental Base of Chinese Academy of Agricultural Sciences (Beijing, China). After setting the eggs, the incubator temperature was raised from 25°C to 37.8°C at a rate of 3°C per hour.…”

“…Moreover, nutrient deficit at the late phase of incubation may limit the development of embryo and post-hatch growth performance of chickens ( Ohta et al., 1999 ). After hatching, modern broilers may fast within 36 to 72 h ( Zhang et al., 2018a ). Delayed feeding increases malnutrition ( Kadam et al., 2013 ) and hinders the maturation of the intestine of chicks ( Gao et al., 2017a ), which negatively affects the development of the small intestine, such as decrease in the villus area, crypt size, crypt proliferation, number of crypt per villus, and rate of enterocyte migration ( Geyra et al., 2001a ).…”

Effects of source and level of in ovo-injected vitamin D3 on the hatchability and serum 25-hydroxycholecalciferol concentrations of Ross 708 broilers

“…The poultry industry commercially uses in ovo injection against Marek's disease. In addition, several laboratories have conducted research to determine effects of the in ovo injection of various nutrients including glucose ( Salmanzadeh, 2012 , Salmanzadeh, 2012 ) and L-ascorbic acid ( Zhang et al., 2018 ), to increase the hatchability and BW and reduce the feed conversion ratio of broilers.…”

Günlük Bıldırcın Civcivlerinde Maternal Hipoksiyanın Uyardığı Çift Yönlü Morfolojik Özelliklerin Gelişme Stabilitesi ve Civciv Gelişimi Üzerine İn Ovo Enjeksiyon Aracılığı ile Antioksidanların Etkileri

“…In the current study, the highest the residual yolk sac weight and the lowest proventriculus + gizzard weight were obtained by IO AA injection to amniotic sac, shown that chicks were less nutrientabsorbing from residual yolk sac as a sign of the effect of hypoxic-stress, though there was no a negative effect on chick weight. Similarly, Zhang et al (2018) had reported that effect of IO administration of different AA dosages to broiler hatching eggs did not eliminate the negative effects of a 48-hour post-hatch holding time on yolk sac utilization. The results of this study indicated that the day-old quail chicks from AA, which was an important energy source, were the more less utilization of nutrients from yolk sac and did not improve digestive system efficiency.…”