Effects of In Ovo Administration of DHEA on Lipid Metabolism and Hepatic Lipogenetic Genes Expression in Broiler Chickens During Embryonic Development

Zhao, Sumei; Ma, Haitian; Zou, Shanmei

doi:10.1007/s11745-007-3068-y

Cited by 29 publications

(25 citation statements)

References 45 publications

(45 reference statements)

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“…Repeated experiments confirmed that b-actin was stable and reliable as an internal reference for the real-time PCR, and it was widely used in some published articles (Zhao et al, 2007;Hu et al, 2008;Chen et al, 2010). The other primers used (FAS, PPAR-a) were determined according to the published guidelines (Zhao et al, 2007;Collin et al, 2009) …”

Section: Treatment and Dietsmentioning

confidence: 83%

“…Reverse transcription was performed using the method of Zhao et al (2007). An aliquot of complementary DNA sample was mixed with 25 ml SYBR_Green PCR Master Mix (Takara, Japan) in the presence of 10 pmol of each forward and reverse primers for b-actin (used as an internal control; GenBank accession no.…”

Section: Treatment and Dietsmentioning

confidence: 99%

“…The other primers used (FAS, PPAR-a) were determined according to the published guidelines (Zhao et al, 2007;Collin et al, 2009) …”

Section: Treatment and Dietsmentioning

confidence: 99%

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Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

Chen

Wang

Kong

et al. 2011

Animal

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The effects of pyruvate (Pyr), creatine pyruvate (Cr-Pyr) and creatine (Cr) on lipid and protein metabolism were compared in broiler chickens. A total of 400 1-day-old male birds (Aconred) were allocated to four groups, each of which included four replicates (25 birds per replicate). Treatments consisted of unsupplemented basal diet (Control), basal diet containing 2% Pyr, basal diet containing 3% Cr and basal diet containing 5% Cr-Pyr. Cr-Pyr and Pyr significantly decreased the hepatic triglyceride and serum total cholesterol concentration (P , 0.01). Cr-Pyr markedly increased the serum non-esterified fatty acid and highdensity lipoprotein cholesterol concentrations (P , 0.05), whereas the expression of carnitine palmitoyl transferase I (P , 0.05) and peroxisome proliferators-activated receptor-a (P , 0.01) mRNA in the liver were both decidedly enhanced in the Cr-Pyr group. The relative leg muscle weight was higher in the Cr-Pyr group than in the control group, whereas the serum uric acid content and hepatic glutamic-oxaloacetic transaminase activity were lower in the Cr-Pyr and Cr groups (P , 0.05), respectively. Muscle insulin-like growth factor I (P , 0.05) expression was enhanced, and the myostatin (P , 0.01) mRNA level was reduced in both the Cr-Pyr and Cr groups. In addition, Cr-Pyr did not alter body weight or the feed conversion ratio. These results indicate that, compared with Pyr and Cr alone, Cr-Pyr has a bifunctional role in broiler chickens, in that it influences both lipid and protein metabolism.Keywords: creatine pyruvate, pyruvate, creatine, lipolysis, protein synthesis ImplicationsIn this study, the administration of creatine pyruvate (Cr-Pyr, a new compound, which contains pure pyruvic acid molecularly bound to pure pharmaceutical grade creatine at a concentration ratio of 40 : 60) was compared with the administration of Pyr or Cr alone for its effect on lipid and protein metabolism in broilers. The insights gained from this study will provide a better understanding of the mechanisms involved in the Cr-Pyr effect on broiler chickens. Further clarity will help the industry to avoid obesity-related problems during production and explore new ways to improve muscle growth in broilers.

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Section: Treatment and Dietsmentioning

confidence: 83%

Section: Treatment and Dietsmentioning

confidence: 99%

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Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

Chen

Wang

Kong

et al. 2011

Animal

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“…Therefore, DHEA might reduce the fat deposition by reducing the TG content in the adipose tissue in rats under a high-fat diet. DHEA treatment alters the activities of a lot of enzymes in the liver that are involved in lipid metabolism (Zhao et al 2007), which could be one of the mechanisms of reducing fat deposition in animal after DHEA administration. The present study showed that FAS mRNA level, the key gene in liver for de novo synthesis of fatty acids, was significantly decreased in the 25 and 50 mg DHEA treatment groups as compared to high-fat diet group.…”

Section: Discussionmentioning

confidence: 99%

“…Total RNA was extracted from liver and adipose samples u sing TRIZOL reagent (TaKaRa Inc., Dalian, China) according to the manufacturer's protocol. Reverse transcription was performed using the method of Zhao et al (2007). An aliquot of cDNA sample was mixed with 25 μl SYBR ® Green PCR Master Mix (TaKaRa Inc.), in the presence of 10 pmol of each forward and reverse primers for β-actin (used as an internal control), fatty acid synthesis (FAS), sterol response element binding protein-1 (SREBP-1), acyl-CoA oxidase (ACO), peroxisome proliferator-activated receptor-α (PPARα), liver carnitine palmitoyl transferase-1 (LCPT-1) or hormone sensitive lipase (HSL) mRNA (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Long-term administration of DHEA prevents fat deposition in rats fed a high-fat diet

Chen¹,

Kang²,

Li³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:The effects of dehydroepiandrosterone (DHEA) on lipid metabolism and lipogenic gene mRNA expression in rats subjected to a high-fat diet were determined. Totally 75 rats were randomly divided into 5 groups: control group 1 fed a normal diet (NCG), and groups 2-5 fed a high-fat diet with 0 (HCG), 25 (HLG), 50 (HMG), 100 (HHG) mg DHEA per kg body weight via gavage once a day for 8 weeks, respectively. DHEA significantly decreased body weight in HMG group as compared with HCG group (P < 0.05). Hepatic triglyceride and total cholesterol contents were decreased in HMG and HHG groups (P < 0.05), and hepatic lipase activity in HMG group was higher (P < 0.01) than in HCG group. Fatty acid synthesis (FAS) mRNA level was decreased in HLG and HHG groups (P < 0.01), and sterol response element binding protein-1 (SREBP-1) mRNA level was decreased in HMG and HHG groups when compared with HCG group (P < 0.01). Acyl-CoA oxidase (ACO) and liver carnitine palmitoyl transferase-1 (LCPT-1) mRNA abundance was decreased in HLG and HHG groups (P < 0.01), whereas hormone sensitive lipase (HSL) mRNA level was increased in HMG group as compared with HCG group (P < 0.05). These results indicated that long-term administration of DHEA reduced the synthesis of endogenous triglycerides by inhibiting SREBP-1 and FAS expression, and augmented the lipolysis of exogenous triglycerides through enhancing HSL expression, which eventually led to reduced fat deposition in rats fed a high-fat diet.

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The effect of dehydroepiandrosterone on lipogenic gene mRNA expression in cultured primary chicken hepatocytes

Tang¹,

Ma²,

Huang³

et al. 2009

Euro J Lipid Sci & Tech

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The effects of dehydroepiandrosterone (DHEA) on lipogenic gene mRNA expression were examined using cultured primary chicken hepatocytes. Cell samples were equilibrated to culture conditions for 24 h and then exposed to DHEA (1, 10 and 100 mM) dissolved in DMSO. The expression of sterol regulatory element-binding protein-1 (SREBP-1) mRNA in cultured primary hepatocytes did not vary with time, except for a significant decrease in gene expression when cells were treated with 10 or 100 mM DHEA for either 1 or 2 h. A similar tendency toward decreased gene expression was evident for acetyl CoA carboxylase. The expression of peroxisome proliferator-activated receptor a (PPARa) and carnitine palmitoyl transferase I mRNA was dramatically enhanced subsequent to treatment with 10 or 100 mM DHEA for periods of time from 6 to 48 h, while there was a decrease in the expression of PPARa in the presence of 10 mM DHEA after 1 and 2 h of exposure, and in the presence of 100 mM DHEA after 2 h of exposure. Hepatocytes treated with 10 or 100 mM DHEA contained more mitochondria and mitochondria with a higher electron density than did untreated hepatocytes. Furthermore, cell survival was significantly inhibited by treatment with 100 mM DHEA at 24, 48 and 72 h. In contrast, 1 mM DHEA administration significantly increased cell survival after 72 h; however, 10 mM DHEA treatment had no pronounced effect on cell survival. Overall, the results reported here indicate that DHEA accelerates lipid catabolism by direct regulation of hepatic gene expression and by induction of changes in hepatocyte mitochondria.

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Effects of In Ovo Administration of DHEA on Lipid Metabolism and Hepatic Lipogenetic Genes Expression in Broiler Chickens During Embryonic Development

Cited by 29 publications

References 45 publications

Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

Long-term administration of DHEA prevents fat deposition in rats fed a high-fat diet

The effect of dehydroepiandrosterone on lipogenic gene mRNA expression in cultured primary chicken hepatocytes

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