Effects of immobilization of Actinobacillus succinogenes on efficiency of bio-succinic acid production from glycerol

Bumyut, Apirak; Champreda, Verawat; Singhakant, Chatchawal; Kanchanasuta, Suwimon

doi:10.1007/s13399-020-01069-2

Cited by 9 publications

(1 citation statement)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, the fermentation of free A.succinogenes 130Z cells in a mixed carbon sources from PKC hydrolysate may cause hemicellulose catabolic repression 24 . Alternative strategies have been put forth including immobilization system which enables cell-entrapment in a feasible condition, hence promoting high cell aggregation, cell density and substrate consumption 25 . At the same time, cell entrapment may induce cell-transfer resistance due to the limitation of nutrient diffusion through the support matrix.…”

Section: Introductionmentioning

confidence: 99%

Biotechnological enhancement of lactic acid conversion from pretreated palm kernel cake hydrolysate by Actinobacillus succinogenes 130Z

Rahim

Luthfi

Bukhari

et al. 2023

Sci Rep

View full text Add to dashboard Cite

The aim of this study was to establish an improved pretreatment and fermentation method i.e. immobilized cells for high recovery of fermentable sugars from palm kernel cake (PKC) and its effects on fermentability performance by Actinobacillus succinogenes 130Z in the conversion of the fermentable sugar to lactic acid. The effects of oxalic acid concentrations (1–6% w/v) and residence times (1–5 h) on the sugar recovery were initially investigated and it was found that the highest mannose concentration was 25.1 g/L at the optimum hydrolysis conditions of 4 h and 3% (w/v) oxalic acid. The subsequent enzymatic saccharification of the pretreated PKC afforded the highest enzymatic digestibility with the recovered sugars amounting to 25.18 g/L and 9.14 g/L of mannose and glucose, respectively. Subsequently, the fermentability performance of PKC hydrolysate was evaluated and compared in terms of cultivation phases (i.e. mono and dual-phases), carbonate loadings (i.e. magnesium and sodium carbonates), and types of sugars (i.e. glucose and mannose). The highest titer of 19.4 g/L lactic acid was obtained from the fermentation involving A. succinogenes 130Z in dual-phase cultivation supplemented with 30 g/L of magnesium carbonate. Lactic acid production was further enhanced by using immobilized cells with coconut shell-activated carbon (CSAC) of different sizes (A, B, C, and D) in the repeated batch cultivation of dual-phase fermentation producing 31.64 g/L of lactic acid. This work sheds light on the possibilities to enhance the utilization of PKC for lactic acid production via immobilized A. succinogenes 130Z.

show abstract

Section: Introductionmentioning

confidence: 99%