Effects of immobilization and partial denervation on the differentiation of muscle fiber types in the zebrafish, Brachydanio rerio

Raamsdonk, W. van; Pool, C.W.; Heyting, C.; teKronnie, G.; Veeken, K.

doi:10.1007/bf00301879

Cited by 12 publications

(12 citation statements)

References 19 publications

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“…Indeed, the myotomal musculature of teleosts consists mainly of two clearly distinguishable layers of fibres. Red (slow oxydative) fibres which are superficially located, and white (fast glycolytic) fibres which occupy the deep portion of the fish trunk musculature [23]. Red (slow) muscle fibres are specialized for sustained use, while white (fast) muscle fibres are specialized in short forceful of activity [23].…”

Section: Discussionmentioning

confidence: 99%

“…Red (slow oxydative) fibres which are superficially located, and white (fast glycolytic) fibres which occupy the deep portion of the fish trunk musculature [23]. Red (slow) muscle fibres are specialized for sustained use, while white (fast) muscle fibres are specialized in short forceful of activity [23]. The functional diversity of the red and white fibres is reflected in their histochemical [23,24] and immunohistochemical properties [25].…”

Section: Discussionmentioning

confidence: 99%

“…Red (slow) muscle fibres are specialized for sustained use, while white (fast) muscle fibres are specialized in short forceful of activity [23]. The functional diversity of the red and white fibres is reflected in their histochemical [23,24] and immunohistochemical properties [25]. Furthermore, the expression of different myosin isoform in these two fibre types has been also established [25,26].…”

Section: Discussionmentioning

confidence: 99%

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A gene with homology to myogenin is expressed in developing myotomal musculature of the rainbow trout and in vitro during the conversion of myosatellite cells to myotubes

1995

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We report the cloning of a new trout myogenic cDNA which encodes helix-loop-helix protein homologous to the myogenic factor myogenin. Northern analyses indicate that trout myogenin (Tmyogenin) transcripts accumulate in large amounts in the myotomal musculature of embryos and frys. In adults, transcripts concentrate within the thin lateral layer of red (slow oxydative) muscle fibres. They are present only in low amounts in white (fast glycolytic) muscle fibres which constitute the major part of the trunk musculature. Using an in vitro myogenesis system, we observed that the trout myogenin encoding gene is not activated until myosatellite cells fuse to generate multinocleated myotubes, indicating that Tmyogenin lies downstream of muscle determination factors. All these observations show that in a major taxinomic group like teleosts, a gene with homology to myogenin exists. Its activation during myogenesis suggests that it acts as a major developmental regulator of muscle differentiation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A gene with homology to myogenin is expressed in developing myotomal musculature of the rainbow trout and in vitro during the conversion of myosatellite cells to myotubes

1995

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“…Van Raamsdonk and colleagues measured myoseptal shape and changes in muscle-fibre orientations at a limited number of sites (Van Raamsdonk et al, 1979;Van Raamsdonk et al, 1982). Interventions of normal mobility patterns prevented normal development of myomere shape and muscle-fibre orientations.…”

Section: Form and Function During Developmentmentioning

confidence: 99%

A functional analysis of myotomal muscle-fibre reorientation in developing zebrafishDanio rerio

Leeuwen

Meulen

Schipper

et al. 2008

Journal of Experimental Biology

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SUMMARYThe fast muscle fibres in the anterior trunk of teleost fish are primarily responsible for large amplitude undulatory swimming motions. Previous theoretical studies suggested that the near-helical arrangement of these fibres results in a (fairly) uniform distribution of fibre strain and work output during swimming. However, the underlying simplifications of these studies precluded unequivocal support for this hypothesis. We studied the fast muscle-fibre reorientation and the concomitant myotomal strain variance in a body segment near the anus during larval and juvenile development in the zebrafish. From 2 to 4 days post fertilization (d.p.f.), the measured angles between the muscle fibres and the longitudinal axis of the zebrafish were small. Yet, onset of a near-helical muscle-fibre arrangement was recognized. Juveniles of 51·d.p.f. have larger mean fibre angles and already possess the near-helical pattern of adult teleosts. We present a model that computes the distribution of the strain along the muscle fibres from measured muscle-fibre orientations, body curvature and prescribed tissue deformations. We selected the most extreme body curvatures, which only occur during fast starts and turning manoeuvres. Using the model, we identified the (nonlinear) tissue deformations that yield the least variance in the muscle-fibre strain. We show that simple beam theory cannot reliably predict the strain distribution: it results in very small strains and negligible work output of the most medial fibres. In our model, we avoided these functional limitations by adding a shear deformation to the simple beam deformation. At 2·d.p.f., the predicted variance in the muscle-fibre strain for the shear deformation optimized for strain uniformity is fairly small, due to the small variation in the fibre distances to the medial plane that is caused by the relatively large spinal cord and notochord. The predicted minimal strain variance increases sharply from 2·d.p.f. to 3·d.p.f., remains relatively large at 4·d.p.f., but decreases again considerably at 15 and 39·d.p.f. The 51·d.p.f. stage exhibits the smallest variance in the fibre strains (for the identified optimal deformation), in spite of the widely varying muscle-fibre distances to the medial plane. The non-linear nature of the body deformations with the least strain variance implies an interesting optimization constraint: the juvenile muscle-fibre arrangement results in small predicted spatial strain variations at large-amplitude body curvatures, at the (modest) expense of a large coefficient of variation for small curvatures. We conclude that larval fish rapidly change their muscle-fibre orientations (probably in response to mechanical signals). Within the theoretically examined plausible range of deformations, the closest correspondence to a uniform strain field was found for the juvenile stage. Supplementary material available online at

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“…Discussion Altered tissue loading as a regulating factor in embryonic development has been the focus of relatively few studies. Some examples are endurance training (Bagatto et al, 2001) and immobilization by agar embedding (Van Raamsdonk et al, 1982) of zebrafish embryos and laser ablation to investigate local tissue forces in dorsal closure Drosophila embryos (Hutson et al, 2003). Altered tissue loading in adult tissues has received more attention because of its relevance for medicine, sports, aquaculture and space flight.…”

Section: Gene Expression Levelsmentioning

confidence: 99%

Effects of decreased muscle activity on developing axial musculature innicb107mutant zebrafish (Danio rerio)

Meulen

Schipper

Leeuwen

et al. 2005

Journal of Experimental Biology

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SUMMARY The present paper discusses the effects of decreased muscle activity (DMA)on embryonic development in the zebrafish. Wild-type zebrafish embryos become mobile around 18 h post-fertilisation, long before the axial musculature is fully differentiated. As a model for DMA, the nicb107mutant was used. In nicb107 mutant embryos, muscle fibres are mechanically intact and able to contract, but neuronal signalling is defective and the fibres are not activated, rendering the embryos immobile. Despite the immobility, distinguished slow and fast muscle fibres developed at the correct location in the axial muscles, helical muscle fibre arrangements were detected and sarcomere architecture was generated. However, in nicb107 mutant embryos the notochord is flatter and the cross-sectional body shape more rounded, also affecting muscle fibre orientation. The stacking of sarcomeres and myofibril arrangement show a less regular pattern. Finally, expression levels of several genes were changed. Together, these changes in expression indicate that muscle growth is not impeded and energy metabolism is not changed by the decrease in muscle activity but that the composition of muscle is altered. In addition, skin stiffness is affected. In conclusion, the lack of muscle fibre activity did not prevent the basal muscle components developing but influenced further organisation and differentiation of these components.

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Effects of immobilization and partial denervation on the differentiation of muscle fiber types in the zebrafish, Brachydanio rerio

Cited by 12 publications

References 19 publications

A gene with homology to myogenin is expressed in developing myotomal musculature of the rainbow trout and in vitro during the conversion of myosatellite cells to myotubes

A gene with homology to myogenin is expressed in developing myotomal musculature of the rainbow trout and in vitro during the conversion of myosatellite cells to myotubes

A functional analysis of myotomal muscle-fibre reorientation in developing zebrafishDanio rerio

Effects of decreased muscle activity on developing axial musculature innicb107mutant zebrafish (Danio rerio)

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