Effects of imidacloprid exposure on Chironomus riparius Meigen larvae: Linking acetylcholinesterase activity to behaviour

Azevedo-Pereira, Henrique M.V.S.; Lemos, Marco F.L.; Soares, Amadeu M.V.M.

doi:10.1016/j.ecoenv.2011.03.018

Cited by 78 publications

(37 citation statements)

References 41 publications

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“…Behavioral impairments of C . riparius larvae exposed to thiacloprid or imidacloprid have also been found by Langer-Jaesrich et al [13] and Azevedo-Pereira et al [30, 31]. However, effect concentrations of thiacloprid were much higher in our study compared to the literature.…”

Section: Discussionsupporting

confidence: 81%

Nano-sized Al2O3 reduces acute toxic effects of thiacloprid on the non-biting midge Chironomus riparius

et al. 2017

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This study focuses on interactions between nanoparticles and a pesticide. The aim was to investigate how nano-sized aluminum oxide (410 nm) can alter the toxic effects of thiacloprid, even if no sorption between particles and the insecticide takes place. Thus, our study investigated a rather unexplored interaction. We conducted our research with larvae of Chironomus riparius and used thiacloprid as test substance as its toxicity to C. riparius is well described. The used nano-Al2O3 particles where chosen due to their suitable properties. For testing the acute effects of the interaction, we exposed larvae to thiacloprid (0.5, 1.0, 2.0, and 5.0 μg/L) and nano-Al2O3 (300 and 1000 mg/L), either solely or in binary mixtures. While thiacloprid resulted in elevated mortality, nano-Al2O3 solely did not exert any effects. Moreover, we observed an aggregation of nano-Al2O3 within the lumen of the intestinal tract of the larvae. Further results showed a significantly reduced mortality of fourth instar larvae when they were exposed to mixtures of nanoparticles and the pesticide, compared to thiacloprid alone. With increasing nano-Al2O3 concentration, this effect became gradually stronger. Additionally, chemical analyses of internal thiacloprid concentrations implicate reduced uptake of thiacloprid in animals exposed to mixtures. However, as larvae exposed to thiacloprid concentrations > 0.5 μg/L showed severe convulsions, independent of the presence or concentration of nano-Al2O3, we assume that nano-Al2O3 leads to a delay of mortality and does not entirely prevent it. As sorption measurements on pristine or defecated nano-Al2O3 did not reveal any sorptive interaction with thiacloprid, we can exclude sorption-based reduction of thiacloprid bioavailability as a mechanism behind our results. Even though we used test substances which might not co-occur in the environment in the tested concentrations, our study gives evidence for an interaction besides adsorption, which is important to generally understand how nanoparticles might affect biota.

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Section: Discussionsupporting

confidence: 81%

Nano-sized Al2O3 reduces acute toxic effects of thiacloprid on the non-biting midge Chironomus riparius

et al. 2017

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“…AChE had also been identified in most invertebrates, including mollusc [31], arthropoda [40], platyhelminthes [41], annelida [42] and nematoda [43]. AChE was also reported to be influencing many physiological behaviors in these invertebrates, including locomotion [43,44,45], feeding [43,44], egg laying [43], male mating [43], embryo development [46] and digestive activity [31]. However, the immunomodulation, genomics and proteomics of AChE is still unclear in invertebrates.…”

Section: Resultsmentioning

confidence: 99%

GENOMICS OF ACETYLCHOLINESTERASE (AChE) ENZYME AND ITS RELATED RNA CONTENT IN THE BRAIN OF RATTUS NORVEGICUS: A PROSPECTIVE STUDY

Rajesh¹,

B²,

B³

et al. 2017

IJAR

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Inhibition of acetylcholinesterase (AChE), the metabolizing enzyme of acetylcholine is evolving as the most important therapeutic target for development of cognitive enhancers. However, AChE activity in brain has not been properly evaluated on the basis of sex. In the present study, AChE expression was investigated in different brain areas of cerebrum and cerebellum in male and female Rattus norvegicus. On comparing male and female genders, increased AChE activity was seen in cerebrum and cerebellum of female Rattus norvegicus. However, no significant change in AChE activity was found between cerebrum and cerebellum within the same male and female. Thus it appears that sex alters AChE activity in different brain regions (G4 isoform) that may vary in male and female. Sequence analysis revealed that highest divergence was found in between male cerebrum and female cerebrum (11.9) and least divergence was found in between male cerebellum and female cerebellum (6.4) with control AChE NM_EF534897

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“…The latter is responsible for proper nervous system functioning (Azevedo-Pereira et al 2011). Paralysis, which was mainly observed in organisms exposed to higher concentration of malathion, can have serious ecological consequences.…”

Section: Discussionmentioning

confidence: 99%

“…For this reason, the biomarker approach has become increasingly more popular. Its use is based on the assumption that low concentrations of toxic substances cause cellular responses in organisms before harmful effects can be observed in larger scales (Azevedo-Pereira et al 2011;Domingues et al 2010;Park et al 2012).…”

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confidence: 99%

Low malathion concentrations influence metabolism in Chironomus sancticaroli (Diptera, Chironomidae) in acute and chronic toxicity tests

Rebechi¹,

Richardi²,

Vicentini³

et al. 2014

Rev. Bras. entomol.

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ABSTRACT. Low malathion concentrations influence metabolism in Chironomus sancticaroli (Diptera, Chironomidae) in acute and chronic toxicity tests. Organophosphate compounds are used in agro-systems, and in programs to control pathogen vectors. Because they are continuously applied, organophosphates often reach water sources and may have an impact on aquatic life. The effects of acute and chronic exposure to the organophosphate insecticide malathion on the midge Chironomus sancticaroli are evaluated. To that end, three biochemical biomarkers, acetylcholinesterase (AChE), alpha (EST-␣) and beta (EST-␤) esterase were used. Acute bioassays with five concentrations of malathion, and chronic bioassays with two concentrations of malathion were carried out. In the acute exposure test, AChE, EST-␣ and EST-␤ activities declined by 66, 40 and 37%, respectively, at 0.251 g L -1 and more than 80% at 1.37, 1.96 and 2.51 g L -1 . In chronic exposure tests, AChE and EST-␣ activities declined by 28 and 15% at 0.251 g L -1 . Results of the present study show that low concentrations of malathion can influence larval metabolism, indicating high toxicity for Chironomus sancticaroli and environmental risk associated with the use of organophosphates.

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Effects of imidacloprid exposure on Chironomus riparius Meigen larvae: Linking acetylcholinesterase activity to behaviour

Cited by 78 publications

References 41 publications

Nano-sized Al2O3 reduces acute toxic effects of thiacloprid on the non-biting midge Chironomus riparius

Nano-sized Al2O3 reduces acute toxic effects of thiacloprid on the non-biting midge Chironomus riparius

GENOMICS OF ACETYLCHOLINESTERASE (AChE) ENZYME AND ITS RELATED RNA CONTENT IN THE BRAIN OF RATTUS NORVEGICUS: A PROSPECTIVE STUDY

Low malathion concentrations influence metabolism in Chironomus sancticaroli (Diptera, Chironomidae) in acute and chronic toxicity tests

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