Effects of ice-binding protein from Leucosporidium on the cryopreservation of boar sperm*

Park, Sang Hyoun; Oh, Keon Bong; Ock, Sun‐A; Byun, Sung June; Lee, Hwi-Cheul; Kumar, Suresh; Lee, Sung Gu; Woo, and Jae-Seok

doi:10.12750/jet.2018.33.3.185

Cited by 2 publications

(1 citation statement)

References 39 publications

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“…Several studies have attempted to improve cryopreserved semen quality by supplementing extenders with antioxidants (Luño et al, 2014;Lee and Kim, 2017) and proteins (Park et al, 2018;Robles et al, 2019), and adding saccharides (Pezo et al, 2020). Other researchers have reported that freezing-thawing protocol can affect with diluent type and centrifugation regime (Carvajal et al, 2004;Stuart et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Washing solution and centrifugation affect kinematics of cryopreserved boar semen

Almubarak

Kim

Abdelbagi

et al. 2021

J Anim Reprod Biotechnol

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Cryopreservation is the most effective method for the long-term storage of semen. Furthermore, cryopreservation enhances genetic utilization by facilitating the distribution of desirable genes and control the transmission of certain diseases (Grossfeld et al., 2008). However, the cryopreservation process exposes semen to physical and chemical stress that impairs sperm quality (Hezavehei et al., 2018). Each step of the cryopreservation including dilution, cooling, freezing, and thawing, diminishes the fertilization potential, with approximately 40% to 50% of spermatozoa not even surviving the process (Rath et al., 2009;Watson, 2000). In comparison with fresh semen, insemination with frozen-thawed semen results in lower fertility and farrowing rates by 20% to 30% (Knox, 2015;Yeste et al., 2017). Boar spermatozoa in particular are very susceptible to peroxidative damage because of their high polyunsaturated fatty acid content and low concentrations of cholesterol in their plasma membrane (Mandal

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Section: Discussionmentioning

confidence: 99%

Washing solution and centrifugation affect kinematics of cryopreserved boar semen

Almubarak

Kim

Abdelbagi

et al. 2021

J Anim Reprod Biotechnol

View full text Add to dashboard Cite

show abstract

Improved viability and fertility of frozen-thawed dog sperm using adipose-derived mesenchymal stem cells

Qamar

Fang

Kim

et al. 2020

Sci Rep

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Cryopreservation procedures negatively affect the quality traits of sperm, causing certain changes at structural and molecular levels due to thermal, mechanical, osmotic, and oxidative damage. The objective of this study was to examine the potential of canine adipose-derived mesenchymal stem cells (Ad-MSCs) for providing protection to the dog sperm against cryo-damage. Canine Ad-MSCs were selected on the basis of the significantly higher gene expression for different proteins actively involved in the cell repair including annexin 1 (ANX1), histone H3 (H3) and high mobility group B (HMGB) protein compared to skin fibroblasts. Semen was collected from four healthy dogs by digital manipulation. The washed pooled ejaculates were diluted with buffer 2 (extender) supplemented without Ad-MSCs (Control), with 2.5 × 10 6 Ad-MSCs/mL (Group 1) or with 5 × 10 6 Ad-MSCs/mL (Group 2). Group 1 exhibited significantly higher post-thaw motility, live sperm, intact plasma membrane and normal acrosomes than the other groups. Additionally, Group 1 showed significantly higher expression levels of genes related to the repair of membranes (ANX1, dysferlin; DYSF, and fibronectin; FN1) and chromatin material (H3 and HMGB). Protein expression of ANX1, H 3, and FN1 was also statistically more in Group 1 than in Control. The results confirm that canine Ad-MSCs can effectively preserve the quality of frozenthawed sperm by a reduction in cryoinjury. At an appropriate concentration, Ad-MSCs significantly improve the quality of post-thaw dog sperm. Semen cryopreservation is regarded as the most important step of artificial insemination which is the most widely adopted assisted reproductive technology (ART) in canine practice 1. It can facilitate the storage of the genetic material for an extended period, conserve the elite individual's fertility and serve as a useful tool for preserving the endangered species. In addition, it has greatly benefited the animal-based industry by reducing the cost and the stress associated with transportation, overcoming the quarantine restrictions and breeding issues (e.g., aggressive behavior and size issues) 2. Despite many attempts to improve freezing agents and techniques, reduction in semen quality still remains the major issue associated with freezing procedures. During freezing the quality-related traits of sperm are highly compromised 3. Sperm undergoes certain detrimental changes at the structural and molecular levels as a result of thermal, mechanical, osmotic, and oxidative damage 4,5. The sperm that survives during freezing procedure suffers a reduction in fertility 6 and this has been linked with damage that adversely affects viability, motility, plasma membrane, acrosome and chromatin material 7. In addition, the activation of apoptotic pathways results in the fragmentation of sperm cell DNA 8. These changes ultimately contribute to an overall reduction in the fertility of sperm. Current protective modifications employed to minimize the above mentioned damaging effects involve the use of different ty...

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Effects of ice-binding protein from Leucosporidium on the cryopreservation of boar sperm*

Cited by 2 publications

References 39 publications

Washing solution and centrifugation affect kinematics of cryopreserved boar semen

Washing solution and centrifugation affect kinematics of cryopreserved boar semen

Improved viability and fertility of frozen-thawed dog sperm using adipose-derived mesenchymal stem cells

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