Effects of icariin on the expression of ER, VEGF, and KDR in the endometrial cells of thin endometrium

Le, Aiwen; Shan, Lili; Wang, Z H; Dai, X Y; Xiao, Tian; Zuo, Ru-Juan

doi:10.4238/2015.september.22.19

Cited by 16 publications

(10 citation statements)

References 28 publications

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“…The effect and dosage of PE, estrogen concentration in the body, and functional status of the target organ are all interdependent (Beck et al, 2003). We have previously shown that thin endometria exhibited low expression of ER and VEGF, even though the blood concentration of estrogen in the body remained normal (Le et al, 2015). In this study, VEGF, CD31, and factor VIII were significantly elevated in rats receiving medium and high doses of ICA, which was indicative of neovascularization.…”

Section: Vegf Expressionsupporting

confidence: 48%

An experimental study on the use of icariin for improving thickness of thin endometrium

Aw¹,

Zh²,

Xy³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. This study aimed to investigate the effect of icariin (ICA) on thin endometrium in a rat model. To this end, 6-to 8-weekold female Sprague Dawley rats (105) were randomly divided into 7 groups: untreated, vehicle-treated (lavage with NaCl), high-dose ICA (lavage with ICA at 200 mg•kg -1 •day -1 ), medium-dose ICA (lavage ICA at 100 mg•kg ), sham model (injected with NaCl at uterus horn), and sample group. To induce thin endometrium, rats of all groups (except shammodel) were injected with 95% ethanol via the uterine horn. Each group underwent its respective treatment for 3 estrous cycles, after which 5 rats from each group were sacrificed, and endometrial thickness was measured. The expression of CD31, factor VIII, vascular endothelial growth factor (VEGF), cytokeratin (CK), and vimentin were detected via immunohistochemistry. The results showed that CD31, factor VIII, and VEGF were primarily expressed in the cytoplasm of endometrial and vascular epithelial cells. No difference in the expression of these factors was detected between the ICA lavage groups and the untreated groups. However, high dose ICA-treated group exhibited significantly higher expression of CD31, factor VIII, and VEGF compared to that in the low dose and vehicle-treated groups. CK and vimentin in the endometrial tissue were significantly higher in the untreated and treatment groups compared to the vehicle-treated group. This study demonstrated that ICA increases thickness of the endometrium, and it may modulate expression of VEGF, CD31, and factor VIII.

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Section: Vegf Expressionsupporting

confidence: 48%

An experimental study on the use of icariin for improving thickness of thin endometrium

Aw¹,

Zh²,

Xy³

et al. 2017

Genet. Mol. Res.

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“…The growth and development of the bone cannot be achieved and sustained unless from the nutritional support of blood ( Filipowska et al, 2017 ). The VEGF is the critical pro-angiogenic factor for vascular formation ( Zhang et al, 2013 ; Le et al, 2015 ). Thiram appeared to cause up-regulation of VEGF expression, suggesting that it has proangiogenic activity ( Rath et al, 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Icariin Ameliorate Thiram-Induced Tibial Dyschondroplasia via Regulation of WNT4 and VEGF Expression in Broiler Chickens

Zhang

Mehmood

et al. 2018

Front. Pharmacol.

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Tibial dyschondroplasia (TD) is main bone problem in fast growing poultry birds that effect proximal growth plate (GP) of tibia bone. TD is broadly defined as non-vascularized and non-mineralized, and enlarged GP with tibia bone deformation and lameness. Icariin (Epimedium sagittatum) is a traditional Chinese medicine, which is commonly practiced in the treatment of various bone diseases. Recently, many researcher reports about the beneficial effects of icariin in relation to various types of bone conditions but no report is available about promoting effect of icariin against TD. Therefore, current study was conducted to explore the ameliorating effect of icariin in thiram-induced TD chickens. A total of 180 broiler chicks were equally distributed in three groups; control, TD induced by thiram (50 mg/kg), and icariin group (treated with icariin @10 mg/kg). All groups were administered with normal standard diet ad libitum regularly until the end of experiment. The wingless-type member 4 (WNT4) and vascular endothelial growth factor (VEGF) genes and proteins expression were analyzed by quantitative real-time polymerase chain reaction and western blot analysis respectively. Tibial bone parameters, physiological changes in serum, antioxidant enzymes, and chicken growth performance were determined to assess advantage and protective effect of the medicine in broiler chicken. The expression of WNT4 was decreased while VEGF increased significantly (P < 0.05) in TD affected chicks. TD enhanced the GP, lameness, and irregular chondrocytes, while reduced the liver function, antioxidant enzymes in liver, and performance of chickens. Icariin treatment up-regulated WNT4 and down-regulated VEGF gene and protein expressions significantly (P < 0.05), restored the GP width, increased growth performance, corrected liver functions and antioxidant enzymes levels in liver, and mitigated the lameness in broiler chickens. In conclusion, icariin administration recovered GP size, normalized performance and prevented lameness significantly. Therefore, icariin treatments are encouraged to reduce the incidence of TD in broiler chickens.

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“…Several previous studies have shown that angiogenesis is essential for tendon-bone healing by detecting CD31 and VEGF expression in the tendon-bone junction (19–21) [17]. ICA has also been shown to promote angiogenesis [52,53,54,55]. Chung et al investigated the molecular effect of ICA on angiogenesis and demonstrated that ICA stimulates angiogenesis by activating the MEK/ERK and PI3K/Akt/eNOS-dependent signal pathways in human endothelial cells [52].…”

Section: Discussionmentioning

confidence: 99%

“…Xin et al studied the effect of ICA on diabetic retinopathy in a rat model and found a significantly increased expression of VEGF in the retinal vessels of diabetic rats after treatment by ICA [55]. In 2015, Le et al conducted an in vitro study and demonstrated that the endometrium may be thickened by ICA treatment, by increasing the expression levels of VEGF in endometrial cells [53]. Similarly, in our study, the histological results showed that ICA clearly enhanced angiogenesis, compared with the control group.…”

Section: Discussionmentioning

confidence: 99%

Icariin Promotes Tendon-Bone Healing during Repair of Rotator Cuff Tears: A Biomechanical and Histological Study

Zhang

Wang

et al. 2016

IJMS

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To investigate whether the systematic administration of icariin (ICA) promotes tendon-bone healing after rotator cuff reconstruction in vivo, a total of 64 male Sprague Dawley rats were used in a rotator cuff injury model and underwent rotator cuff reconstruction (bone tunnel suture fixation). Rats from the ICA group (n = 32) were gavage-fed daily with ICA at 0.125 mg/g, while rats in the control group (n = 32) received saline only. Micro-computed tomography, biomechanical tests, serum ELISA (calcium; Ca, alkaline phosphatase; AP, osteocalcin; OCN) and histological examinations (Safranin O and Fast Green staining, type I, II and III collagen (Col1, Col2, and Col3), CD31, and vascular endothelial growth factor (VEGF)) were analyzed two and four weeks after surgery. In the ICA group, the serum levels of AP and OCN were higher than in the control group. More Col1-, Col2-, CD31-, and VEGF-positive cells, together with a greater degree of osteogenesis, were detected in the ICA group compared with the control group. During mechanical testing, the ICA group showed a significantly higher ultimate failure load than the control group at both two and four weeks. Our results indicate that the systematic administration of ICA could promote angiogenesis and tendon-bone healing after rotator cuff reconstruction, with superior mechanical strength compared with the controls. Treatment for rotator cuff injury using systematically-administered ICA could be a promising strategy.

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Effects of icariin on the expression of ER, VEGF, and KDR in the endometrial cells of thin endometrium

Cited by 16 publications

References 28 publications

An experimental study on the use of icariin for improving thickness of thin endometrium

An experimental study on the use of icariin for improving thickness of thin endometrium

Icariin Ameliorate Thiram-Induced Tibial Dyschondroplasia via Regulation of WNT4 and VEGF Expression in Broiler Chickens

Icariin Promotes Tendon-Bone Healing during Repair of Rotator Cuff Tears: A Biomechanical and Histological Study

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