Effects of ibuprofen on a pig Pseudomonas ARDS model

Lee, C.C.; Sugarman, H. J.; Tatum, James L.

doi:10.1016/0883-9441(88)90060-3

Cited by 7 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pulmonary hypertension, lung edema, increased pulmonary shunting, systemic hypotension, and hypoxemia, after endotoxin administration in calves, sheep, pigs, and cats, are attenuated by indomethacin (cyclooxygenase inhibitor), ibuprofen (cyclooxygenase inhibitor with preferential inhibition of thromboxane synthesis), and prostacyclin (short-term vasodilator). Attenuation by glucocorticoids, however, is limited to improvements in hemoconcentration and cardiac output Sturgess, 1974,1976;Anderson et al, 1975;Traber et al, 1981;Dehring et al, 1983b;Steinberg et al, 1983;Kopolovic et al, 1984Kopolovic et al, , 1986Olson et al, 1985;Teague et al, 1985;Albertine and Staub, 1986;Brigham and Meyrick, 1986;Lee et al, 1986). These findings document a substantial contribution of bioactive lipids to the generation of lung injury after endotoxin application.…”

Section: Regulatory Properties Of Pimsmentioning

confidence: 72%

“…Low doses of endotoxin (15 micrograms per kilogram body weight) do not elicit increased pulmonary artery pressure in dogs (Hales et al, 1981;Jacobs et al, 1982) but will cause severe pulmonary hypertension and respiratory distress in calves, sheep, goats, pigs, and cats (Kuida et al, 1961;Reeves et al, 1972;Maxie et al, 1974;Anderson et al, 1975;Olson et al, 1985;Brigham and Meyrick, 1986;Winn et al, 1987). Intravenous application of bacteria (P-hemolytic streptococci, Escherichia coli, and Pseude monas aeruginosa), endotoxin, zymosan-activated (complement-activated) plasma, or liposomes results in an immediate rise of pulmonary arterial pressure in calves, sheep, goats, cats, or pigs (Kuida et al, 1961;Tikoff et al, 1966;Anderson et al, 1975;Parratt and Sturgess, 1976;Crocker et al, 1980;Dehring et al, 1983bDehring et al, , 1984Brigham, 1983,1984;Borg et al, 1984;Niehaus et al, 1984;Parratt et al, 1984;Olson et al, 1985;Teague et al, 1985;Brigham and Meyrick, 1986;Lee et al, 1986;Meadow et al, 1986;Miyamoto et al, 1986). Pulmonary hypertension, lung edema, increased pulmonary shunting, systemic hypotension, and hypoxemia, after endotoxin administration in calves, sheep, pigs, and cats, are attenuated by indomethacin (cyclooxygenase inhibitor), ibuprofen (cyclooxygenase inhibitor with preferential inhibition of thromboxane synthesis), and prostacyclin (short-term vasodilator).…”

Section: Regulatory Properties Of Pimsmentioning

confidence: 96%

See 1 more Smart Citation

Pulmonary intravascular macrophages in domestic animal species: Review of structural and functional properties

Winkler

1988

Am. J. Anat.

154

View full text Add to dashboard Cite

In dogs, laboratory animals, and man, the clearance of bacteria and particulates from blood occurs predominantly in hepatic Kupffer cells and splenic macrophages. In contrast, removal of blood-borne particulates in calves, sheep, goats, cats, and pigs occurs predominantly in pulmonary intravascular macrophages (PIMs). Review of recent studies indicates that PIMs are a resident cell population, junctionally adherent to the capillary endothelium of lungs and morphologically similar to hepatic Kupffer cells. PIMs are a pulmonary constituent of the mononuclear phagocyte system with respect to secretory, endocytic, and functional properties. Differentiated PIMs are rare in newborn pigs, and the majority of cells closely apposed to capillary endothelium consists of monocytes, which are occasionally in mitosis. In 7-day-old and older pigs, most cells apposed to capillary endothelium have characteristics of differentiated PIMs. This suggests a monocytic origin of PIMs in pigs. Perinatal colonization of lung capillaries by monocytes and their subsequent differentiation into PIMs represent a component of postnatal lung development. Estimates of relative PIM numbers in ovine and porcine lung parenchyma suggest cell densities similar to that of rat hepatic Kupffer cells. Apart from phagocytic properties, PIMs participate in the removal and disintegration of aged and impaired blood cells. After phagocytic stimulation, isolated PIMs secrete oxygen radicals, which are essential for microbicidal function. Similarly, by secreting bioactive lipids, stimulated PIMs may contribute to regulation of pulmonary hemodynamics. After receiving minute amounts of bacterial endotoxin, pulmonary injury is pronounced in sheep, calves, pigs, and cats, but not in laboratory animals and dogs. This presumably is related to the secretion of bioactive lipids by PIMs.

show abstract

Section: Regulatory Properties Of Pimsmentioning

confidence: 72%

Section: Regulatory Properties Of Pimsmentioning

confidence: 96%

Pulmonary intravascular macrophages in domestic animal species: Review of structural and functional properties

Winkler

1988

Am. J. Anat.

154

View full text Add to dashboard Cite

show abstract

“…This early increase in PVR can be abrogated by the administration of inhib itors of cyclooxygenase (i.e. nonsteroidal anti-inflammatory drugs) [ 15], Some of these will shunt the arachidonic acid cascade over to the lipoxygenase pathway, however, and thereby aggravate the second phase of the pulmonary response to endotoxin [16], With the combination of drugs used in the present study, we have abolished the early increase in PVR without specifically interfering with the arachidonic acid cascade, implying that other mediators may be of importance in the porcine model. Regretfully, this improve ment was of relatively short duration.…”

Section: Discussionmentioning

confidence: 99%

Combined Drug Therapy in Porcine Endotoxemia

Naess

Roeise²,

Stadaas³

et al. 1990

Eur Surg Res

View full text Add to dashboard Cite

In order to investigate the importance of potential mediators of pathophysiologic derangements in endotoxemia, we have examined the effects of the combined administration of antagonists against histamine, serotonin and endorphins in a porcine model of endotoxemia. The treatment regimen significantly reduced the increase in pulmonary artery pressure, pulmonary vascular resistance and systemic arterial pressure seen in the early stages of endotoxemia. Also, cardiac output was better maintained. However, the hemodynamic performance after an observation period of 5 h was not statistically different from untreated animals. The treatment regimen did not hinder the activation of the kallikrein-kinin and fibrinolytic systems of plasma, which was evident in both treated and untreated animals, and could not counteract the increase in hematocrit or leukopenia seen in this model. This study shows that the combined blocking of histamine, serotonin and endorphines is not enough to abrogate the detrimental effects of endotoxin in a porcine model.

show abstract

“…Early studies by Lee. et al discovered that ibuprofen inhibited pulmonary vasoconstriction and bronchiolar constriction in pigs infected with P. aeruginosa (18). Moreover, in an acute P. aeruginosa pulmonary infection in mice, ibuprofen decreased the recruitment of granulocytes to airways and suppressed lung inflammation (19).…”

Section: Introductionmentioning

confidence: 99%

Ibuprofen-mediated potential inhibition of biofilm development and quorum sensing inPseudomonas aeruginosa

Dai

TianQi²,

Wang

et al. 2019

Preprint

View full text Add to dashboard Cite

23Pseudomonas aeruginosa is one of the leading causes of opportunistic and 24 hospital-acquired infections worldwide. The infection with P. aeruginosa is frequently 25 linked with clinical treatment difficulties given drug resistance and abuse of 26 antibiotics. Ibuprofen, a widely used non-steroidal anti-inflammatory drug, has been 27 previously reported to exert antimicrobial activity, although the specific mechanism of 28 its action requires additional investigation. Given the regulation effects on quorum 29 sensing (QS), we hypothesized that inhibition of P. aeruginosa with ibuprofen is 30 linked with the QS systems. First, we assessed the action of ibuprofen in P. 31 aeruginosa by measuring CFU. The antimicrobial activity of ibuprofen was evaluated 32 2 by crystal violent staining and acridine orange staining at various drug concentrations 33 (0, 50, 75, and 100 μg/mL). Moreover, the effect of ibuprofen on different QS 34 virulence factors, such as pyocyanin, elastase, protease, and rhamnolipids, was 35 assessed revealing a concentration-dependent decrease (P<0.05). The effect of 36 ibuprofen was confirmed by liquid chromatography/mass spectrometry analysis of 37 3-oxo-C 12 -HSL and C 4 -HSL production. In addition, qRT-PCR results identified 38 significant suppression of Las and Rhl gene expression after 18 hours of treatment 39 with ibuprofen (P<0.05), with the most significant suppression observed at the 40 concentration of 75 μg/mL. Functional complementation with exogenous 41 3-oxo-C 12 -HSL and C 4 -HSL suggested that C 4 -HSL can recover the production of 42 virulence factors and biofilm formation in P. aeruginosa. Molecular docking of 43 ibuprofen with QS-associated proteins revealed high binding affinity. In summary, the 44 results suggest that ibuprofen is a candidate drug for the treatment of clinical 45 infections with P. aeruginosa. 46 Keywords: P. aeruginosa, ibuprofen, antimicrobial activity, quorum sensing, N-acyl 47 homoserine lactones 48 49 Introduction 50 Pseudomonas aeruginosa, the most common Gram-negative non-fermentative 51 bacteria, is one of the leading causes of opportunistic and hospital-acquired infections 52 worldwide(1). The infection can cause serious complications in patients with burns, 53 cystic fibrosis, or in immunocompromised patients with respiratory infections, sepsis, 54 osteomyelitis, endocarditis, and urinary tract infections (UTIs) (2). The infection with 55 P. aeruginosa has been liked with difficulties in clinical treatment because of 56 significant resistance of the bacterium to antibiotics (3), which is further aggravated 57 by the worldwide abuse of antibiotics. Specifically, every year approximately 700,000 58 people die from antibiotic resistance worldwide, which can likely increase to 10 59 million by 2050 if no action is taken to reduce drug resistance or to develop new 60 antibiotics. In 2017, the World Health Organization (WHO) published a list of 61 drug-resistant bacteria threating the lives of people which needs require for which 62 3 new antibio...

show abstract

Effects of ibuprofen on a pig Pseudomonas ARDS model

Cited by 7 publications

References 0 publications

Pulmonary intravascular macrophages in domestic animal species: Review of structural and functional properties

Pulmonary intravascular macrophages in domestic animal species: Review of structural and functional properties

Combined Drug Therapy in Porcine Endotoxemia

Ibuprofen-mediated potential inhibition of biofilm development and quorum sensing inPseudomonas aeruginosa

Contact Info

Product

Resources

About