Effects of Lactobacillus rhamnosus on asthma with an adoptive transfer of dendritic cells in mice

Abstract

Aim: This study was designed to investigate whether the protective effects of Lactobacillus rhamnosus (Lcr35) on allergic asthma are associated with the adoptive transfer of dendritic cells (DCs) and regulatory T cells (Tregs), using a mouse experimental model of asthma. Methods and Results: BALB/c mice were orally administered Lcr35 or intravenously treated with in vivo Lcr35-treated DCs daily and were then sensitized and challenged with ovalbumin (OVA) in accordance with a model of asthma protocol. Both the oral application of Lcr35 and intravenous administration of Lcr35-treated DCs suppressed all aspects of the asthmatic response, including bronchial hyperresponsiveness (BHR), total cell counts in the bronchoalveolar lavage (BAL) fluid, the production of OVAspecificimmunoglobulin E (IgE), and pulmonary eosinophilic inflammation. The mechanism of action of Lcr35 is related to Tregs, which suppress the Th2 response in the respiratory organs, and this is mediated by DCs in the mouse model of asthma. Conclusions: These results confirm that the mechanism underlying the effects of Lcr35 on asthma involves the adoptive transfer of DCs. Significance and Impact of the Study: This finding broadens the possibility that Lcr35 has potential as an alternative therapeutic approach to the treatment of human asthma.

Clinical efficacy and mechanism of probiotics in allergic diseases

“…In addition, some studies have shown this effect only in tumors19). Our research confirms that DCs are involved in the suppression of asthma by probiotics following the transfer of mature DCs in mice56).…”

Oral administration ofLactobacillus caseivarietyrhamnosuspartially alleviates TMA-induced atopic dermatitis in mice through improving intestinal microbiota

“…The findings of this study are concordance with those reported in other recent studies (Kim et al . , ) and provide additional support for the preventive effects of LCR35 against allergic disease.…”

A multistrain probiotic formulation attenuates skin symptoms of atopic dermatitis in a mouse model through the generation of CD4+Foxp3+T cells

“…Tregs are known to modulate the upregulation of Th1 and Th2, thereby inhibiting the progression of inflammation in allergic conditions (24). Many studies have suggested the involvement of Tregs in controlling various aspects of AD (25–27). Ingested probiotics are recognized by pattern recognition receptors, such as TLRs on DCs (28), that are essential for immunological homeostasis in the gut and that play an important role in allergic diseases; therefore, CD4 + Foxp3 + Tregs are increased in MLNs (10, 27).…”

“…Many studies have suggested the involvement of Tregs in controlling various aspects of AD (25–27). Ingested probiotics are recognized by pattern recognition receptors, such as TLRs on DCs (28), that are essential for immunological homeostasis in the gut and that play an important role in allergic diseases; therefore, CD4 + Foxp3 + Tregs are increased in MLNs (10, 27). In this study, analysis of Tregs in MLNs by using flow cytometry showed that the percentage of CD4 + Foxp3 + Tregs increased significantly in the GI7-H group compared with that of the control group, whereas no changes were observed in the control and GI7-L groups (Fig.…”