Effects ofin UteroExposure to Di(n-butyl) Phthalate for Estrogen Receptors α, β, and Androgen Receptor of Leydig Cell on Rats

Wakui, Shin; Shirai, Masaru; Motohashi, Masaya; Mutou, Tomoko; Oyama, Noriko; Wempe, Michael F.; Takahashi, Hiroyuki; Inomata, Tomo; Ikegami, Machiko; Endou, Hitoshi; Asari, Masao

doi:10.1177/0192623313502879

Cited by 28 publications

(35 citation statements)

References 55 publications

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“…These abnormalities are consistent with inhibiting fetal testosterone production (Scott et al, 2009). Moreover, decreases in testicular or serum testosterone levels in juvenile male rats (≤ postnatal days 35) (Shultz et al, 2001;Mylchreest et al, 2002;Fisher et al, 2003;Fisher, 2004) and in adult male rats Wakui et al, 2013Wakui et al, , 2014Giribabu et al, 2014) were described after in utero DBP exposure. Leydig cells (LC) are the cells that synthesize testosterone.…”

Section: Introductionsupporting

confidence: 59%

“…Dose solutions were prepared fresh every morning and administered at 9:00 am. The regimen was based upon previous studies that demonstrated adverse effects of DBP on fetal male rats at 100 mg/kg/day (Mylchreest et al, 2000;Barlow and Foster, 2003;Shirai et al, 2013;Wakui et al, 2013Wakui et al, , 2014. Offspring were weighed and sexed at birth.…”

Section: Chemicals Animals and Compound Dosingmentioning

confidence: 99%

“…The non-aqueous extract portion was decanted, dried overnight in a fume hood, and reconstituted in assay buffer. The assay limit of detection (LOD) was 40 pg/testis Wakui et al, 2013Wakui et al, , 2014.…”

Section: Testosterone Level Analysismentioning

confidence: 99%

“…After isolation, the quality of RNA samples were assessed using an Agilent 2100 bioanalyzer (Agilent Technologies Inc., Santa Clara, CA, USA) and an RNA 6000 LabChip Kit (Agilent Technologies) (Wakui et al, , 2014.…”

Section: Rna Isolationmentioning

confidence: 99%

“…Consequently, efforts in our laboratories have been focused on studying phthalate exposure in female rats and subsequent effects to their offspring. We have mainly studied di(n-butyl) phthalate (DBP), the phthalate prepared from butanol Wakui et al, 2013Wakui et al, , 2014. DBP is widely used as a plasticizer and the general population appears to be exposed to it in disproportionately higher amounts compared to other phthalates (Blount et al, 2000;Barlow et al, 2003Barlow et al, , 2004.…”

Section: Introductionmentioning

confidence: 99%

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Retraction: In utero-exposed di(n-butyl) phthalate induce dose dependent, age-related changes of morphology and testosterone-biosynthesis enzymes/associated proteins of Leydig cell mitochondria in rats

Motohashi

Wempe

Mutou³

et al. 2016

J. Toxicol. Sci.

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-Female pregnant Sprague-Dawley rats were intragastrically (ig) administered di(n-butyl) phthalate (DBP) at four doses (0, 10, 50 and 100 mg/kg) during gestation days (GD) 12-21 (n = 5 per group). The age-related morphological changes of Leydig cell mitochondrion (LC-Mt) and testosterone biosynthesis enzymes/associated genes/proteins expression levels were investigated. As compared to the control (no DBP), the 10 mg, and 50 mg DBP dose groups, the 100 mg DBP dose group at weeks 5 and 7 showed a significant amount of small LC-Mt. Thereafter, from weeks 9 to 17, the LC-Mt size and quantity in the100 mg DBP dose group increased and became statistically similar to the other dose groups; hence, dose and time-dependent LC-Mt changes were observed. Throughout the study, the 100 mg DBP dose group had significantly lower testosterone levels. In addition, the 100 mg DBP dose group displayed lower StAR (StAR, steroidogenic acute regulatory protein) and P450scc (CYP11a1, cholesterol side-chain cleavage enzyme) levels at weeks 5 and 7, but they became statistically similar to all other dose groups at weeks 9 to 17; in contrast, the SR-B1 (Sarb1, scavenger receptor class B member 1) levels were similar for all DBP dose groups. The rats in utero 100 mg DBP /kg/day (GD 12-21) exposure results from this study indicate a dose-dependent, age-related morphological change in LC-Mt which are linked to reductions in testosterone biosynthesis genes / proteins expression, specifically StAR and P450scc.

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Section: Introductionsupporting

confidence: 59%

Section: Chemicals Animals and Compound Dosingmentioning

confidence: 99%

Section: Testosterone Level Analysismentioning

confidence: 99%

Section: Rna Isolationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Retraction: In utero-exposed di(n-butyl) phthalate induce dose dependent, age-related changes of morphology and testosterone-biosynthesis enzymes/associated proteins of Leydig cell mitochondria in rats

Motohashi

Wempe

Mutou³

et al. 2016

J. Toxicol. Sci.

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Competitive adsorption of phthalate esters on marine surface sediments: kinetic, thermodynamic, and environmental considerations

Mohammadian

Ghanemi

Nikpour

2016

Environ Sci Pollut Res

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In this study, the sorption behavior of six widely used phthalate esters (PEs) on marine sediments was investigated. The adsorption of PEs was fast and reached the equilibrium within 6 h. The forward and backward rate constants of all PEs on sediments were calculated. Several kinds of kinetic and thermodynamic models have been investigated; the pseudo-second-order model and the partition isotherm model were best fitted to the adsorption behavior of PEs. The rate-limiting step of sorption was controlled by the film diffusion mechanism. After treating sediments with HO, the partition coefficients of all PEs were significantly reduced and indicated that the amorphous organic carbon has a major role in adsorption process. The negative values of ΔH° and ΔG° for these compounds showed that the sorption process is exothermic and spontaneous. The adsorption capacities of all PEs were slightly influenced by increasing the salinity from 0 to 40 g L. These research findings have a prime importance on assessment of the fate and transport of PEs in seawater-sediment systems.

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Association of phthalate exposure with anthropometric indices and blood pressure in first-grade children

Yang

et al. 2018

Environ Sci Pollut Res

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We aimed to assess the relationship of urine phthalate metabolite concentrations with anthropometric indices, and blood pressure in first-grade children. We detected 11 phthalate metabolites in urine and estimated anthropometric indices, including skinfold measurements, waist circumference (WC), and body mass index (BMI) in 276 children aged 6-8 years. Multivariate linear regression models were used to assess the associations between urinary phthalate metabolite levels, and anthropometric and blood pressure indices in a gender-specific manner. In boys, a 1-ng/mL increase in monobenzyl phthalate (MBzP) concentration was associated with a 0.027-cm decrease in the skinfold measurement (95% confidence interval [CI], - 0.053 to 0.001), whereas a 1-ng/mL increase in mono-ethyl-phthalate (MEP) concentration was associated with a 0.016-mm Hg decrease in systolic blood pressure (95% CI, - 0.031 to 0.001). MBzP, mono-(2-ethyl-5-carboxypentyl) phthalate (MECPP), and MEOHP concentrations were also inversely associated with WC. However, in girls, MEP concentrations were positively associated with chest measurements, but were inversely associated with WC. A 1-ng/mL increase in monomethyl phthalate concentrations was associated with a 0.039-cm increase in skinfold measurements (95% CI, 0.002 to 0.076), whereas a 1-ng/mL increase in MECPP concentrations was associated with a 0.050 cm decrease in skinfold measurements (95% CI, - 0.095 to - 0.005). In this exploratory, cross-sectional analysis, we identified various interesting associations between different phthalate metabolite levels and anthropometric indices, which suggest that some of phthalate metabolite should be considered in addition to the prevalence rates of overweight and obesity.

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Effects ofin UteroExposure to Di(n-butyl) Phthalate for Estrogen Receptors α, β, and Androgen Receptor of Leydig Cell on Rats

Cited by 28 publications

References 55 publications

Retraction: <i>In utero</i>-exposed di(<i>n</i>-butyl) phthalate induce dose dependent, age-related changes of morphology and testosterone-biosynthesis enzymes/associated proteins of Leydig cell mitochondria in rats

Retraction: <i>In utero</i>-exposed di(<i>n</i>-butyl) phthalate induce dose dependent, age-related changes of morphology and testosterone-biosynthesis enzymes/associated proteins of Leydig cell mitochondria in rats

Competitive adsorption of phthalate esters on marine surface sediments: kinetic, thermodynamic, and environmental considerations

Association of phthalate exposure with anthropometric indices and blood pressure in first-grade children

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