Effects of <i>Eucommia ulmoides</i> Extract on Longitudinal Bone Growth Rate in Adolescent Female Rats

Kim, Ji Young; Lee, Jeong-Il; Song, Mi‐Kyung; Lee, Donghun; Song, Jungbin; Kim, Soo Young; Park, Juyeon; Choi, Ho-Young; Kim, Ho-Cheol

doi:10.1002/ptr.5195

Cited by 25 publications

(19 citation statements)

References 23 publications

(21 reference statements)

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“…P. umbrosa at doses of 100 and 300 mg/kg was shown to increase the bone growth rate to 380.9 and 381.5 μm/day in the proximal tibial growth plate, respectively. The bone growth rate in the control group was 358.2 μm/day in accordance with the results reported previously [41]. Tetracycline gets deposited in newly formed bones, causing a fluorescent line corresponding to the injection, and the distance between the chondro-osseous junction and the fluorescent line indicates the growth rate, which is the length of bone growth during a certain period of time [37].…”

Section: Discussionsupporting

confidence: 87%

Effects of Phlomis umbrosa Root on Longitudinal Bone Growth Rate in Adolescent Female Rats

et al. 2016

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Abstract:This study aimed to investigate the effects of Phlomis umbrosa root on bone growth and growth mediators in rats. Female adolescent rats were administered P. umbrosa extract, recombinant human growth hormone or vehicle for 10 days. Tetracycline was injected intraperitoneally to produce a glowing fluorescence band on the newly formed bone on day 8, and 5-bromo-2 1 -deoxyuridine was injected to label proliferating chondrocytes on days 8-10. To assess possible endocrine or autocrine/paracrine mechanisms, we evaluated insulin-like growth factor-1 (IGF-1), insulin-like growth factor binding protein-3 (IGFBP-3) or bone morphogenetic protein-2 (BMP-2) in response to P. umbrosa administration in either growth plate or serum. Oral administration of P. umbrosa significantly increased longitudinal bone growth rate, height of hypertrophic zone and chondrocyte proliferation of the proximal tibial growth plate. P. umbrosa also increased serum IGFBP-3 levels and upregulated the expressions of IGF-1 and BMP-2 in growth plate. In conclusion, P. umbrosa increases longitudinal bone growth rate by stimulating proliferation and hypertrophy of chondrocyte with the increment of circulating IGFBP-3. Regarding the immunohistochemical study, the effect of P. umbrosa may also be attributable to upregulation of local IGF-1 and BMP-2 expressions in the growth plate, which can be considered as a GH dependent autocrine/paracrine pathway.

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Section: Discussionsupporting

confidence: 87%

Effects of Phlomis umbrosa Root on Longitudinal Bone Growth Rate in Adolescent Female Rats

et al. 2016

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“…Finally, the sections were photographed with a microscope. The percentage of labelled chondrocytes was calculated by counting stained and total chondrocytes in two parallel columns using Image J software [ 3 ].…”

Section: Methodsmentioning

confidence: 99%

“…The growth plate consists of three distinct zones: the resting, proliferative, and hypertrophic zones. Chondrocytes in the growth plate are differentiated from the top of the resting zone to the bottom of the hypertrophic zone, and the middle proliferative zone is the major location for chondrocyte proliferation for bone elongation [ 2 , 3 ]. Growth and differentiation of the growth plate are regulated by growth hormone (GH) and cell-signalling polypeptides such as insulin-like growth factor (IGF)-1 and bone morphogenetic protein (BMP)-2 [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Huang Bai (Phellodendri Cortex) on bone growth and pubertal development in adolescent female rats

Lee

et al. 2018

Chin Med

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BackgroundTo evaluate the effects of Huang Bai (Phellodendron amurense) on growth and maturation in adolescent female rats.MethodsFemale Sprague–Dawley rats (28 days old; n = 72) were divided into six daily treatment groups: control (distilled water), Huang Bai (100 and 300 mg/kg), recombinant human GH (rhGH; 20 μg/kg), estradiol (1 μg/kg), and triptorelin (100 μg). Body weight, food intake, and vaginal opening were measured daily from postnatal day (PND) 28 to PND 43. Tetracycline (20 mg/kg) was injected on PND 41. After sacrifice on PND 43, the ovaries and uterus were weighed, and the tibias were fixed in 4% paraformaldehyde. Decalcified and dehydrated tibias were sectioned at a thickness of 40 μm, and sectioned tissues were examined with a fluorescence microscope. Insulin-like growth factor (IGF)-1 and bone morphogenetic protein (BMP)-2 were detected using immunohistochemistry.ResultsRelative to controls, body weight was higher in the triptorelin group. Bone growth rate increased in the Huang Bai 100 mg/kg (354.00 ± 31.1 μm/day), rhGH (367.10 ± 27.11 μm/day), and triptorelin (374.50 ± 25.37 μm/day) groups. Expression of IGF-1 and BMP-2 in the hypertrophic zone was higher in all experimental groups. Vaginal opening occurred earlier in the estradiol group (PND 33.58 ± 1.62) than in controls and later in the triptorelin group (PND > 43). Ovarian and uterine weights were lower in the oestradiol and triptorelin groups. However, Huang Bai had nonsignificant effects on vaginal opening and the weights of ovaries and the uterus.ConclusionsHuang Bai stimulated bone growth by upregulating IGF-1 and BMP-2 in the growth plate. However, it had no effect on pubertal development.Electronic supplementary materialThe online version of this article (10.1186/s13020-017-0156-7) contains supplementary material, which is available to authorized users.

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“…In ovariectomized rats fed with Eucommiae Cortex extract, the femur biomechanical quality and trabecular microarchitecture were improved without hyperplastic effect on uterus [234]. When adolescent female rats were treated with the bark extract, promotion of longitudinal bone growth was observed, with increased BMP-2 and IGF-1 expressions in the proliferative and hypertrophic zones [235]. In a disuse-induced osteoporosis model of hind-limb suspended rats, the Eucommia bark extract could prevent bone loss as indicated by decreased levels of bone turnover markers; it enhanced the biomechanical bone strength and prevented the deterioration of trabecular bone microarchitecture [236].…”

Section: Natural Products With Osteoprotective and Related Activitiesmentioning

confidence: 99%

Natural Products from Chinese Medicines with Potential Benefits to Bone Health

2016

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Abstract:Osteoporosis is a progressive, systemic bone disorder characterized by loss of bone mass and microstructure, leading to reduced bone strength and increased risk of fracture. It is often associated with reduced quality of life and other medical complications. The disease is common in the aging population, particularly among postmenopausal women and patients who receive long-term steroidal therapy. Given the rapid growth of the aging population, increasing life expectancy, the prevalence of bone loss, and financial burden to the healthcare system and individuals, demand for new therapeutic agents and nutritional supplements for the management and promotion of bone health is pressing. With the advent of global interest in complementary and alternative medicine and natural products, Chinese medicine serves as a viable source to offer benefits for the improvement and maintenance of bone health. This review summarizes the scientific information obtained from recent literatures on the chemical ingredients of Chinese medicinal plants that have been reported to possess osteoprotective and related properties in cell-based and/or animal models. Some of these natural products (or their derivatives) may become promising leads for development into dietary supplements or therapeutic drugs.

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Effects of Eucommia ulmoides Extract on Longitudinal Bone Growth Rate in Adolescent Female Rats

Cited by 25 publications

References 23 publications

Effects of Phlomis umbrosa Root on Longitudinal Bone Growth Rate in Adolescent Female Rats

Effects of Phlomis umbrosa Root on Longitudinal Bone Growth Rate in Adolescent Female Rats

Effects of Huang Bai (Phellodendri Cortex) on bone growth and pubertal development in adolescent female rats

Natural Products from Chinese Medicines with Potential Benefits to Bone Health

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